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Protein

Deoxyhypusine synthase

Gene

Dhps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei137 – 1371NADBy similarity
Binding sitei238 – 2381NADBy similarity
Binding sitei243 – 2431SpermidineBy similarity
Binding sitei283 – 2831NAD; via amide nitrogenBy similarity
Binding sitei288 – 2881SpermidineBy similarity
Active sitei329 – 3291NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1095NADBy similarity
Nucleotide bindingi131 – 1333NADBy similarity
Nucleotide bindingi308 – 3092NADBy similarity
Nucleotide bindingi342 – 3432NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • deoxyhypusine biosynthetic process from spermidine Source: MGI
  • glucose homeostasis Source: MGI
  • peptidyl-lysine modification to peptidyl-hypusine Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • protein homotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:Dhps
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2683592. Dhps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Deoxyhypusine synthasePRO_0000314944Add
BLAST

Proteomic databases

EPDiQ3TXU5.
MaxQBiQ3TXU5.
PaxDbiQ3TXU5.
PeptideAtlasiQ3TXU5.
PRIDEiQ3TXU5.

PTM databases

iPTMnetiQ3TXU5.

Expressioni

Gene expression databases

BgeeiQ3TXU5.
CleanExiMM_DHPS.
ExpressionAtlasiQ3TXU5. baseline and differential.
GenevisibleiQ3TXU5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077733.

Structurei

3D structure databases

ProteinModelPortaliQ3TXU5.
SMRiQ3TXU5. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1372Spermidine bindingBy similarity
Regioni314 – 3163Spermidine bindingBy similarity
Regioni323 – 3297Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
HOVERGENiHBG000852.
InParanoidiQ3TXU5.
KOiK00809.
OMAiIWEMGKW.
OrthoDBiEOG73804P.
TreeFamiTF300625.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3TXU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGTPPGAAP SSALAAVLKH SSALPPESAQ VQGYDFNRGV DYHALLDAYR
60 70 80 90 100
TTGFQATNFG RAVQQVNAMI EKKLEPLAVD EDHHADLTQS RRPLTGCTIF
110 120 130 140 150
LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE
160 170 180 190 200
FSLRGKELRE SGINRIGNLL VPNDNYCKFE DWLMPILDQM VLEQNTEGVK
210 220 230 240 250
WTPSKMISRL GKEINNPDSV YYWAHKNHIP VLSPALTDGS LGDMIFFHSY
260 270 280 290 300
KNPGLVLDIV EDLRLINTQA IFAKRSGMII LGGGVVKHHI ANANLMRNGA
310 320 330 340 350
DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RMDAQPVKVY ADASLVFPLL
360
VAETFAQKAD AFRAEKNED
Length:369
Mass (Da):40,642
Last modified:July 27, 2011 - v2
Checksum:i22B835EEE4E9DFAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti340 – 3401Y → C in BAE34820 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159104 mRNA. Translation: BAE34820.1.
AC163703 Genomic DNA. No translation available.
CCDSiCCDS22492.1.
RefSeqiNP_001034603.1. NM_001039514.1.
UniGeneiMm.193086.
Mm.456030.

Genome annotation databases

EnsembliENSMUST00000078665; ENSMUSP00000077733; ENSMUSG00000060038.
GeneIDi330817.
KEGGimmu:330817.
UCSCiuc009mpf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159104 mRNA. Translation: BAE34820.1.
AC163703 Genomic DNA. No translation available.
CCDSiCCDS22492.1.
RefSeqiNP_001034603.1. NM_001039514.1.
UniGeneiMm.193086.
Mm.456030.

3D structure databases

ProteinModelPortaliQ3TXU5.
SMRiQ3TXU5. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077733.

PTM databases

iPTMnetiQ3TXU5.

Proteomic databases

EPDiQ3TXU5.
MaxQBiQ3TXU5.
PaxDbiQ3TXU5.
PeptideAtlasiQ3TXU5.
PRIDEiQ3TXU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078665; ENSMUSP00000077733; ENSMUSG00000060038.
GeneIDi330817.
KEGGimmu:330817.
UCSCiuc009mpf.1. mouse.

Organism-specific databases

CTDi1725.
MGIiMGI:2683592. Dhps.

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
HOVERGENiHBG000852.
InParanoidiQ3TXU5.
KOiK00809.
OMAiIWEMGKW.
OrthoDBiEOG73804P.
TreeFamiTF300625.

Enzyme and pathway databases

UniPathwayiUPA00354.
ReactomeiR-MMU-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

ChiTaRSiDhps. mouse.
PROiQ3TXU5.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TXU5.
CleanExiMM_DHPS.
ExpressionAtlasiQ3TXU5. baseline and differential.
GenevisibleiQ3TXU5. MM.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Spleen and Testis.

Entry informationi

Entry nameiDHYS_MOUSE
AccessioniPrimary (citable) accession number: Q3TXU5
Secondary accession number(s): E9QM48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.