ID   TRM1_MOUSE              Reviewed;         663 AA.
AC   Q3TX08; Q3TBY4;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 2.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=N(2),N(2)-dimethylguanosine tRNA methyltransferase;
DE            EC=2.1.1.32;
DE   AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase;
DE   AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase;
DE   AltName: Full=tRNA(m(2,2)G26)dimethyltransferase;
GN   Name=Trmt1; Synonyms=D8Ertd812e;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and NOD;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=17203969; DOI=10.1021/pr0604155;
RA   Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
RT   "Protein phosphorylation and expression profiling by Yin-yang
RT   multidimensional liquid chromatography (Yin-yang MDLC) mass
RT   spectrometry.";
RL   J. Proteome Res. 6:250-262(2007).
CC   -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC       most tRNAs using S-adenosyl-L-methionine as donor of the methyl
CC       groups (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(2)-methylguanine.
CC   -!- SIMILARITY: Belongs to the TRM1 family.
CC   -!- SIMILARITY: Contains 1 C3H1-type zinc finger.
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DR   EMBL; AK159467; BAE35108.1; -; mRNA.
DR   EMBL; AK171002; BAE42173.1; -; mRNA.
DR   IPI; IPI00321152; -.
DR   UniGene; Mm.275720; -.
DR   PhosphoSite; Q3TX08; -.
DR   Ensembl; ENSMUSG00000001909; Mus musculus.
DR   MGI; MGI:1289155; Trmt1.
DR   HOGENOM; Q3TX08; -.
DR   HOVERGEN; Q3TX08; -.
DR   BRENDA; 2.1.1.32; 244.
DR   ArrayExpress; Q3TX08; -.
DR   Bgee; Q3TX08; -.
DR   GermOnline; ENSMUSG00000001909; Mus musculus.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR002905; TRM_MeTrfase.
DR   InterPro; IPR000571; Znf_CCCH.
DR   PANTHER; PTHR10631; TRM_mtfrase; 1.
DR   Pfam; PF02005; TRM; 1.
DR   Pfam; PF00642; zf-CCCH; 1.
DR   SMART; SM00356; ZnF_C3H1; 1.
DR   TIGRFAMs; TIGR00308; TRM1; 1.
DR   PROSITE; PS50103; ZF_C3H1; 1.
PE   1: Evidence at protein level;
KW   Metal-binding; Methyltransferase; Phosphoprotein;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; Zinc;
KW   Zinc-finger.
FT   CHAIN         1    663       N(2),N(2)-dimethylguanosine tRNA
FT                                methyltransferase.
FT                                /FTId=PRO_0000147672.
FT   ZN_FING     599    626       C3H1-type.
FT   MOD_RES     121    121       Phosphoserine.
FT   MOD_RES     624    624       Phosphoserine (By similarity).
FT   CONFLICT     45     46       FC -> SW (in Ref. 1; BAE35108).
FT   CONFLICT    112    112       D -> E (in Ref. 1; BAE42173).
FT   CONFLICT    175    175       G -> D (in Ref. 1; BAE42173).
FT   CONFLICT    656    656       G -> V (in Ref. 1; BAE42173).
SQ   SEQUENCE   663 AA;  72350 MW;  28D9D7678FED5D55 CRC64;
     MSLARTILWL SRPLRPAHSL CRAQFMERKA QKPPSPPAME NGTRFCEERP PADPVATVTE
     GAAKIVFPSA NEVFYNPVQE FNRDLTCAVI TEFARIHLGA KGIQIKVPGE KDSEKIAVDL
     SDQEEETAGK NENLAPGDWP RTAAVGEICE EGLRVLEGLA ASGLRSIRFA LEVPGLQSVV
     ANDASARAVE LMHRNVELNG VAHLVQPNQA DARMLMYQHQ KAPERFDVID LDPYGSPAPF
     LDAAVQAVSD GGLLCVTCTD MAVLAGNSGE TCYSKYGAMA LKSRACHEMA LRIVLHSLDL
     HANCYQRYIV PLLSISADFY IRVFVRVFTG QAKVKSSASK QALVFQCVGC GAFYLQRLGK
     ASGDPGGRIK FSAACGPPVT PECEHCGQRH QLGGPMWAEP IHDLDFVGRV LDAVTTNPGR
     FHTSMRIQGV LSVVTEELPD VPLYYTLDQL SSTIHCNTPR LLQLRSALLH AGFRVSLSHA
     CKNAVKTDAP PEALWDIMRC WEKECPVKRE RLSESSPAFR ILAVEPRLKA NFNIREDANP
     SSRQRGLKRF QANPEANWGP RPRARPGGKA ASEDLAGRRR LLQNKRKEPA EDPAQRAARL
     KTFPCKRFKE GTCQLGDQCC YSHSPAAPVA SGDIPIEECP ETTTKISPGP KAAAGGIPGP
     GVD
//