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Protein

Metal transporter CNNM2

Gene

Cnnm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg2+ > Co2+ > Mn2+ > Sr2+ > Ba2+ > Cu2+ > Fe2+.1 Publication

Miscellaneous

Shares weak sequence similarity with the cyclin family, hence its name. However, it has no cyclin-like function in vivo.

GO - Molecular functioni

  • ATP binding Source: MGI
  • magnesium ion transmembrane transporter activity Source: MGI

GO - Biological processi

  • magnesium ion homeostasis Source: MGI
  • magnesium ion transport Source: MGI

Keywordsi

Biological processIon transport, Transport

Protein family/group databases

TCDBi9.A.40.3.1 the hlyc/corc (hcc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Metal transporter CNNM2
Alternative name(s):
Ancient conserved domain-containing protein 2
Short name:
mACDP2
Cyclin-M2
Gene namesi
Name:Cnnm2
Synonyms:Acdp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2151054 Cnnm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 250ExtracellularSequence analysisAdd BLAST250
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21
Topological domaini272 – 313CytoplasmicSequence analysisAdd BLAST42
Intramembranei314 – 334HelicalSequence analysisAdd BLAST21
Topological domaini335 – 338CytoplasmicSequence analysis4
Transmembranei339 – 359HelicalSequence analysisAdd BLAST21
Topological domaini360 – 368ExtracellularSequence analysis9
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Topological domaini390 – 875CytoplasmicSequence analysisAdd BLAST486

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62 – 65GCTA → LLLV: Impairs N-terminal cleavage. 1 Publication4
Mutagenesisi62 – 64GCT → LCL: No effect on N-terminal cleavage. 3
Mutagenesisi63 – 65CTA → LTV: No effect on N-terminal cleavage. 3
Mutagenesisi112N → A: Loss of N-glycosylation; 90% decrease of plasma membrane expression. 1 Publication1
Mutagenesisi327N → A: No effect on N-glycosylation. 1 Publication1
Mutagenesisi527N → A: No effect on N-glycosylation. 1 Publication1
Mutagenesisi591N → A: No effect on N-glycosylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002957611 – 875Metal transporter CNNM2Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei761PhosphoserineBy similarity1

Post-translational modificationi

The N-terminus is cleaved within the endoplasmic reticulum. The signal peptidase complex seems to be involved in the processing, but the exact cleavage site has not been identified (PubMed:22399287).1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ3TWN3
PaxDbiQ3TWN3
PeptideAtlasiQ3TWN3
PRIDEiQ3TWN3

PTM databases

iPTMnetiQ3TWN3
PhosphoSitePlusiQ3TWN3
SwissPalmiQ3TWN3

Expressioni

Tissue specificityi

Widely expressed, with highest levels in kidney, lung, spleen and testis. In the kidney, predominantly expressed in the distal convoluted tubule and, at lower levels, in the connecting tubule (at protein level).3 Publications

Inductioni

By low Mg2+ concentration.1 Publication

Gene expression databases

BgeeiENSMUSG00000064105
CleanExiMM_CNNM2
GenevisibleiQ3TWN3 MM

Interactioni

Subunit structurei

Isoform 1 and isoform 2 may interact with each other.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096972

Structurei

Secondary structure

1875
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi430 – 440Combined sources11
Turni441 – 443Combined sources3
Helixi446 – 449Combined sources4
Beta strandi450 – 452Combined sources3
Helixi453 – 455Combined sources3
Beta strandi464 – 466Combined sources3
Helixi467 – 476Combined sources10
Beta strandi479 – 487Combined sources9
Beta strandi491 – 496Combined sources6
Helixi497 – 500Combined sources4
Helixi505 – 507Combined sources3
Helixi511 – 517Combined sources7
Beta strandi523 – 526Combined sources4
Helixi531 – 540Combined sources10
Beta strandi545 – 552Combined sources8
Beta strandi555 – 557Combined sources3
Beta strandi560 – 568Combined sources9
Helixi569 – 577Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P1GX-ray2.60A430-580[»]
4P1OX-ray3.06A/B430-580[»]
5LXQX-ray3.33A/H430-584[»]
5MMZX-ray2.40A430-584[»]
ProteinModelPortaliQ3TWN3
SMRiQ3TWN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 431CNNM transmembranePROSITE-ProRule annotationAdd BLAST181
Domaini450 – 511CBS 1PROSITE-ProRule annotationAdd BLAST62
Domaini518 – 584CBS 2PROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Belongs to the ACDP family.Curated

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2118 Eukaryota
COG1253 LUCA
GeneTreeiENSGT00390000002383
HOGENOMiHOG000231947
HOVERGENiHBG074775
InParanoidiQ3TWN3
KOiK16302
OMAiVMASRMD
OrthoDBiEOG091G02YS
TreeFamiTF101012

Family and domain databases

InterProiView protein in InterPro
IPR000644 CBS_dom
IPR002550 CNNM
PfamiView protein in Pfam
PF00571 CBS, 1 hit
PF01595 DUF21, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS51846 CNNM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TWN3-1) [UniParc]FASTAAdd to basket
Also known as: CNNM2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGCGACEPE VKMAGGQAAA ALPTWKMAAR RSLSARGRGV LQAAAGRLLP
60 70 80 90 100
LLLLSCCWGA GGCTAAGENE ETVIIGLRLE DTNDVSFMEG GALRVSERTR
110 120 130 140 150
VKLRVYGQNI NNETWSRIAF TEHERRRHTP SERGLGGPAP PEPDSGPQRC
160 170 180 190 200
GIRTSDIIIL PHIILNRRTS GIIEIEIKPL RKMEKSKSYY LCTSLSTPAL
210 220 230 240 250
GAGGSGSASG TVGGKGGAGV AGLPPPPWAE TTWIYHDGED TKMIVGEEKK
260 270 280 290 300
FLLPFWLQVI FISLLLCLSG MFSGLNLGLM ALDPMELRIV QNCGTEKEKN
310 320 330 340 350
YAKRIEPVRR QGNYLLCSLL LGNVLVNTTL TILLDDIAGS GLVAVVVSTI
360 370 380 390 400
GIVIFGEIVP QAICSRHGLA VGANTIFLTK FFMMMTFPAS YPVSKLLDCV
410 420 430 440 450
LGQEIGTVYN REKLLEMLRV TDPYNDLVKE ELNIIQGALE LRTKTVEDVM
460 470 480 490 500
TPLRDCFMIT GEAILDFNTM SEIMESGYTR IPVFEGERSN IVDLLFVKDL
510 520 530 540 550
AFVDPDDCTP LKTITKFYNH PLHFVFNDTK LDAMLEEFKK GKSHLAIVQR
560 570 580 590 600
VNNEGEGDPF YEVLGIVTLE DVIEEIIKSE ILDETDLYTD NRTKKKVAHR
610 620 630 640 650
ERKQDFSAFK QTDSEMKVKI SPQLLLAMHR FLATEVEAFS PSQMSEKILL
660 670 680 690 700
RLLKHPNVIQ ELKYDEKNKK APECYLYQRN KPVDYFVLIL QGKVEVEAGK
710 720 730 740 750
EGMKFEASAF SYYGVMALTA SPVPLSLSRT FVVSRTEVLA AGSPGENKSP
760 770 780 790 800
PRPCGLNHSD SLSRSDRIDA MTPTLGSSNN QLSSSFLQVY IPDYSVRALS
810 820 830 840 850
DLQFVKISRQ QYQNALMASR MDKTPQSSDS ENTKIELTLT ELHDGLPDET
860 870
ANLLNEQNCV SHNKANHSLH SEGAI
Length:875
Mass (Da):96,704
Last modified:July 27, 2011 - v3
Checksum:i8074D56C079E0F6C
GO
Isoform 2 (identifier: Q3TWN3-2) [UniParc]FASTAAdd to basket
Also known as: CNNM2b

The sequence of this isoform differs from the canonical sequence as follows:
     721-742: Missing.

Show »
Length:853
Mass (Da):94,436
Checksum:i7B574A06D8B529DC
GO

Sequence cautioni

The sequence AAF86373 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH27387 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH52513 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131S → G in BAC31904 (PubMed:16141072).Curated1
Sequence conflicti131S → G in AAH27387 (PubMed:19468303).Curated1
Sequence conflicti664Y → C in BAE35233 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027081721 – 742Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044400 mRNA Translation: BAC31904.1
AK159616 mRNA Translation: BAE35233.1
AC122442 Genomic DNA No translation available.
AC161865 Genomic DNA No translation available.
BC027387 mRNA Translation: AAH27387.1 Sequence problems.
BC052513 mRNA Translation: AAH52513.1 Different initiation.
AF216961 mRNA Translation: AAF86373.1 Different initiation.
CCDSiCCDS50459.1 [Q3TWN3-1]
CCDS50460.1 [Q3TWN3-2]
RefSeqiNP_001095941.1, NM_001102471.1 [Q3TWN3-2]
NP_291047.2, NM_033569.3 [Q3TWN3-1]
UniGeneiMm.306903

Genome annotation databases

EnsembliENSMUST00000077666; ENSMUSP00000076850; ENSMUSG00000064105 [Q3TWN3-2]
ENSMUST00000099373; ENSMUSP00000096972; ENSMUSG00000064105 [Q3TWN3-1]
GeneIDi94219
KEGGimmu:94219
UCSCiuc008hue.1 mouse [Q3TWN3-1]
uc008huf.1 mouse [Q3TWN3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNNM2_MOUSE
AccessioniPrimary (citable) accession number: Q3TWN3
Secondary accession number(s): A0PJF1
, E9PUH1, Q7TT07, Q8C8V4, Q9JIM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 99 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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