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Protein

Metal transporter CNNM2

Gene

Cnnm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg2+ > Co2+ > Mn2+ > Sr2+ > Ba2+ > Cu2+ > Fe2+.1 Publication

GO - Molecular functioni

  • ATP binding Source: MGI
  • magnesium ion transmembrane transporter activity Source: MGI

GO - Biological processi

  • magnesium ion homeostasis Source: MGI
  • magnesium ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi9.A.40.3.1. the hlyc/corc (hcc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Metal transporter CNNM2
Alternative name(s):
Ancient conserved domain-containing protein 2
Short name:
mACDP2
Cyclin-M2
Gene namesi
Name:Cnnm2
Synonyms:Acdp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2151054. Cnnm2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 250250ExtracellularSequence analysisAdd
BLAST
Transmembranei251 – 27121HelicalSequence analysisAdd
BLAST
Topological domaini272 – 31342CytoplasmicSequence analysisAdd
BLAST
Intramembranei314 – 33421HelicalSequence analysisAdd
BLAST
Topological domaini335 – 3384CytoplasmicSequence analysis
Transmembranei339 – 35921HelicalSequence analysisAdd
BLAST
Topological domaini360 – 3689ExtracellularSequence analysis
Transmembranei369 – 38921HelicalSequence analysisAdd
BLAST
Topological domaini390 – 875486CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 654GCTA → LLLV: Impairs N-terminal cleavage. 1 Publication
Mutagenesisi62 – 643GCT → LCL: No effect on N-terminal cleavage.
Mutagenesisi63 – 653CTA → LTV: No effect on N-terminal cleavage.
Mutagenesisi112 – 1121N → A: Loss of N-glycosylation; 90% decrease of plasma membrane expression. 1 Publication
Mutagenesisi327 – 3271N → A: No effect on N-glycosylation. 1 Publication
Mutagenesisi527 – 5271N → A: No effect on N-glycosylation. 1 Publication
Mutagenesisi591 – 5911N → A: No effect on N-glycosylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Metal transporter CNNM2PRO_0000295761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...)2 Publications
Modified residuei761 – 7611PhosphoserineBy similarity

Post-translational modificationi

The N-terminus is cleaved within the endoplasmic reticulum. The signal peptidase complex seems to be involved in the processing, but the exact cleavage site has not been identified (PubMed:22399287).1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ3TWN3.
PaxDbiQ3TWN3.
PeptideAtlasiQ3TWN3.
PRIDEiQ3TWN3.

PTM databases

iPTMnetiQ3TWN3.
PhosphoSiteiQ3TWN3.
SwissPalmiQ3TWN3.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in kidney, lung, spleen and testis. In the kidney, predominantly expressed in the distal convoluted tubule and, at lower levels, in the connecting tubule (at protein level).3 Publications

Inductioni

By low Mg2+ concentration.1 Publication

Gene expression databases

BgeeiQ3TWN3.
CleanExiMM_CNNM2.
GenevisibleiQ3TWN3. MM.

Interactioni

Subunit structurei

Isoform 1 and isoform 2 may interact with each other.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096972.

Structurei

Secondary structure

1
875
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi430 – 44011Combined sources
Turni441 – 4433Combined sources
Helixi446 – 4494Combined sources
Helixi453 – 4553Combined sources
Beta strandi464 – 4663Combined sources
Helixi467 – 4759Combined sources
Beta strandi479 – 4879Combined sources
Beta strandi491 – 4966Combined sources
Helixi497 – 5004Combined sources
Helixi505 – 5073Combined sources
Helixi511 – 5188Combined sources
Beta strandi524 – 5263Combined sources
Helixi531 – 5399Combined sources
Beta strandi540 – 5423Combined sources
Beta strandi545 – 5528Combined sources
Beta strandi555 – 5584Combined sources
Beta strandi560 – 5689Combined sources
Helixi569 – 5779Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P1GX-ray2.60A430-580[»]
4P1OX-ray3.06A/B430-580[»]
ProteinModelPortaliQ3TWN3.
SMRiQ3TWN3. Positions 429-580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini258 – 430173DUF21Add
BLAST
Domaini450 – 51162CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 58467CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ACDP family.Curated
Contains 2 CBS domains.PROSITE-ProRule annotation
Contains 1 DUF21 domain.Curated

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2118. Eukaryota.
COG1253. LUCA.
GeneTreeiENSGT00390000002383.
HOGENOMiHOG000231947.
HOVERGENiHBG074775.
InParanoidiQ3TWN3.
KOiK16302.
OMAiVMASRMD.
OrthoDBiEOG72ZCD7.
TreeFamiTF101012.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR002550. DUF21.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF01595. DUF21. 1 hit.
[Graphical view]
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TWN3-1) [UniParc]FASTAAdd to basket

Also known as: CNNM2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGCGACEPE VKMAGGQAAA ALPTWKMAAR RSLSARGRGV LQAAAGRLLP
60 70 80 90 100
LLLLSCCWGA GGCTAAGENE ETVIIGLRLE DTNDVSFMEG GALRVSERTR
110 120 130 140 150
VKLRVYGQNI NNETWSRIAF TEHERRRHTP SERGLGGPAP PEPDSGPQRC
160 170 180 190 200
GIRTSDIIIL PHIILNRRTS GIIEIEIKPL RKMEKSKSYY LCTSLSTPAL
210 220 230 240 250
GAGGSGSASG TVGGKGGAGV AGLPPPPWAE TTWIYHDGED TKMIVGEEKK
260 270 280 290 300
FLLPFWLQVI FISLLLCLSG MFSGLNLGLM ALDPMELRIV QNCGTEKEKN
310 320 330 340 350
YAKRIEPVRR QGNYLLCSLL LGNVLVNTTL TILLDDIAGS GLVAVVVSTI
360 370 380 390 400
GIVIFGEIVP QAICSRHGLA VGANTIFLTK FFMMMTFPAS YPVSKLLDCV
410 420 430 440 450
LGQEIGTVYN REKLLEMLRV TDPYNDLVKE ELNIIQGALE LRTKTVEDVM
460 470 480 490 500
TPLRDCFMIT GEAILDFNTM SEIMESGYTR IPVFEGERSN IVDLLFVKDL
510 520 530 540 550
AFVDPDDCTP LKTITKFYNH PLHFVFNDTK LDAMLEEFKK GKSHLAIVQR
560 570 580 590 600
VNNEGEGDPF YEVLGIVTLE DVIEEIIKSE ILDETDLYTD NRTKKKVAHR
610 620 630 640 650
ERKQDFSAFK QTDSEMKVKI SPQLLLAMHR FLATEVEAFS PSQMSEKILL
660 670 680 690 700
RLLKHPNVIQ ELKYDEKNKK APECYLYQRN KPVDYFVLIL QGKVEVEAGK
710 720 730 740 750
EGMKFEASAF SYYGVMALTA SPVPLSLSRT FVVSRTEVLA AGSPGENKSP
760 770 780 790 800
PRPCGLNHSD SLSRSDRIDA MTPTLGSSNN QLSSSFLQVY IPDYSVRALS
810 820 830 840 850
DLQFVKISRQ QYQNALMASR MDKTPQSSDS ENTKIELTLT ELHDGLPDET
860 870
ANLLNEQNCV SHNKANHSLH SEGAI
Length:875
Mass (Da):96,704
Last modified:July 27, 2011 - v3
Checksum:i8074D56C079E0F6C
GO
Isoform 2 (identifier: Q3TWN3-2) [UniParc]FASTAAdd to basket

Also known as: CNNM2b

The sequence of this isoform differs from the canonical sequence as follows:
     721-742: Missing.

Show »
Length:853
Mass (Da):94,436
Checksum:i7B574A06D8B529DC
GO

Sequence cautioni

The sequence AAF86373.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH27387.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH52513.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311S → G in BAC31904 (PubMed:16141072).Curated
Sequence conflicti131 – 1311S → G in AAH27387 (PubMed:19468303).Curated
Sequence conflicti664 – 6641Y → C in BAE35233 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei721 – 74222Missing in isoform 2. 1 PublicationVSP_027081Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044400 mRNA. Translation: BAC31904.1.
AK159616 mRNA. Translation: BAE35233.1.
AC122442 Genomic DNA. No translation available.
AC161865 Genomic DNA. No translation available.
BC027387 mRNA. Translation: AAH27387.1. Sequence problems.
BC052513 mRNA. Translation: AAH52513.1. Different initiation.
AF216961 mRNA. Translation: AAF86373.1. Different initiation.
CCDSiCCDS50459.1. [Q3TWN3-1]
CCDS50460.1. [Q3TWN3-2]
RefSeqiNP_001095941.1. NM_001102471.1. [Q3TWN3-2]
NP_291047.2. NM_033569.3. [Q3TWN3-1]
UniGeneiMm.306903.

Genome annotation databases

EnsembliENSMUST00000077666; ENSMUSP00000076850; ENSMUSG00000064105. [Q3TWN3-2]
ENSMUST00000099373; ENSMUSP00000096972; ENSMUSG00000064105. [Q3TWN3-1]
GeneIDi94219.
KEGGimmu:94219.
UCSCiuc008hue.1. mouse. [Q3TWN3-1]
uc008huf.1. mouse. [Q3TWN3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044400 mRNA. Translation: BAC31904.1.
AK159616 mRNA. Translation: BAE35233.1.
AC122442 Genomic DNA. No translation available.
AC161865 Genomic DNA. No translation available.
BC027387 mRNA. Translation: AAH27387.1. Sequence problems.
BC052513 mRNA. Translation: AAH52513.1. Different initiation.
AF216961 mRNA. Translation: AAF86373.1. Different initiation.
CCDSiCCDS50459.1. [Q3TWN3-1]
CCDS50460.1. [Q3TWN3-2]
RefSeqiNP_001095941.1. NM_001102471.1. [Q3TWN3-2]
NP_291047.2. NM_033569.3. [Q3TWN3-1]
UniGeneiMm.306903.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P1GX-ray2.60A430-580[»]
4P1OX-ray3.06A/B430-580[»]
ProteinModelPortaliQ3TWN3.
SMRiQ3TWN3. Positions 429-580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096972.

Protein family/group databases

TCDBi9.A.40.3.1. the hlyc/corc (hcc) family.

PTM databases

iPTMnetiQ3TWN3.
PhosphoSiteiQ3TWN3.
SwissPalmiQ3TWN3.

Proteomic databases

MaxQBiQ3TWN3.
PaxDbiQ3TWN3.
PeptideAtlasiQ3TWN3.
PRIDEiQ3TWN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077666; ENSMUSP00000076850; ENSMUSG00000064105. [Q3TWN3-2]
ENSMUST00000099373; ENSMUSP00000096972; ENSMUSG00000064105. [Q3TWN3-1]
GeneIDi94219.
KEGGimmu:94219.
UCSCiuc008hue.1. mouse. [Q3TWN3-1]
uc008huf.1. mouse. [Q3TWN3-2]

Organism-specific databases

CTDi54805.
MGIiMGI:2151054. Cnnm2.

Phylogenomic databases

eggNOGiKOG2118. Eukaryota.
COG1253. LUCA.
GeneTreeiENSGT00390000002383.
HOGENOMiHOG000231947.
HOVERGENiHBG074775.
InParanoidiQ3TWN3.
KOiK16302.
OMAiVMASRMD.
OrthoDBiEOG72ZCD7.
TreeFamiTF101012.

Miscellaneous databases

ChiTaRSiCnnm2. mouse.
PROiQ3TWN3.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TWN3.
CleanExiMM_CNNM2.
GenevisibleiQ3TWN3. MM.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR002550. DUF21.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF01595. DUF21. 1 hit.
[Graphical view]
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-875 (ISOFORM 1).
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary gland and Mammary tumor.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 27-36, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Molecular cloning and characterization of the mouse Acdp gene family."
    Wang C.-Y., Yang P., Shi J.-D., Purohit S., Guo D., An H., Gu J.-G., Ling J., Dong Z., She J.-X.
    BMC Genomics 5:7-7(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 176-875 (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Brain.
  6. "Functional characterization of ACDP2 (ancient conserved domain protein), a divalent metal transporter."
    Goytain A., Quamme G.A.
    Physiol. Genomics 22:382-389(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112.
  8. Cited for: DIMERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MEMBRANE TOPOLOGY, MUTAGENESIS OF 62-GLY--ALA-65; ASN-112; ASN-327; ASN-527 AND ASN-591, GLYCOSYLATION AT ASN-112.

Entry informationi

Entry nameiCNNM2_MOUSE
AccessioniPrimary (citable) accession number: Q3TWN3
Secondary accession number(s): A0PJF1
, E9PUH1, Q7TT07, Q8C8V4, Q9JIM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Shares weak sequence similarity with the cyclin family, hence its name. However, it has no cyclin-like function in vivo.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.