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Protein

Trichoplein keratin filament-binding protein

Gene

Tchp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (By similarity). Is a negative regulator of ciliogenesis (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Trichoplein keratin filament-binding protein
Short name:
Protein TCHP
Alternative name(s):
Mitochondrial protein with oncostatic activity
Short name:
Mitostatin
Gene namesi
Name:TchpImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1925082. Tchp.

Subcellular locationi

GO - Cellular componenti

  • apical cortex Source: HGNC
  • centrosome Source: MGI
  • ciliary transition fiber Source: MGI
  • cytoplasm Source: UniProtKB
  • desmosome Source: MGI
  • keratin filament Source: HGNC
  • mitochondrion Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926101 – 497Trichoplein keratin filament-binding proteinAdd BLAST497

Post-translational modificationi

Ubiquitinated. Ubiquitination by the BCR(KCTD17) E3 ubiquitin ligase complex results in proteasomal degradation, and induces ciliogenesis.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ3TVW5.
PaxDbiQ3TVW5.
PRIDEiQ3TVW5.

PTM databases

iPTMnetiQ3TVW5.
PhosphoSitePlusiQ3TVW5.

Expressioni

Tissue specificityi

Expressed in all tissues examined, including brain, liver, small intestine, large intestine, lung and heart. Found concentrated in tubular structures within hepatocytes, and in the apical cortical region and desmosomes of the apical junctional domain in enterocytes of the small intestine. In the hair follicle, localized at the outer root sheath. Also expressed in blood vessels (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000002486.
CleanExiMM_TCHP.
ExpressionAtlasiQ3TVW5. baseline and differential.
GenevisibleiQ3TVW5. MM.

Interactioni

Subunit structurei

Interacts specifically with keratin proteins including, KRT5, KRT6A, KRT8, KRT14, KRT16 and KRT18. Interacts with KCTD17 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3TVW5. 1 interactor.
STRINGi10090.ENSMUSP00000092009.

Structurei

3D structure databases

ProteinModelPortaliQ3TVW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 457Interaction with keratin proteinsBy similarityAdd BLAST386
Regioni258 – 424Trichohyalin/plectin homology domainBy similarityAdd BLAST167

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili67 – 140Sequence analysisAdd BLAST74
Coiled coili166 – 271Sequence analysisAdd BLAST106
Coiled coili327 – 479Sequence analysisAdd BLAST153

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi69 – 466Glu-richSequence analysisAdd BLAST398

Sequence similaritiesi

Belongs to the TCHP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IIAX. Eukaryota.
ENOG410ZEPV. LUCA.
GeneTreeiENSGT00730000111170.
HOGENOMiHOG000006708.
HOVERGENiHBG097262.
InParanoidiQ3TVW5.
KOiK16811.
OMAiTLPSYWC.
OrthoDBiEOG091G0FWB.
PhylomeDBiQ3TVW5.
TreeFamiTF329032.

Family and domain databases

InterProiIPR026773. TCHP.
[Graphical view]
PANTHERiPTHR31183:SF2. PTHR31183:SF2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3TVW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPTLPSYW SSRKHMDLRQ RQHQQEDQFR QQWEQNSRYF RTWDIHNSKQ
60 70 80 90 100
IEWSSKISYQ RSMHAYHCEK MKEEKRKILE LRRERLRELL LEEQDLLAAE
110 120 130 140 150
LDELRLSMGL REQRLREQHQ DLKSAREEQR KLIAERLLYE HWKKNNPKLR
160 170 180 190 200
ELELDLHKKH VINSWATQKE EKKQQEATEK QENKRLENQY AAARREAEAR
210 220 230 240 250
MRVEEERRQL EGRLQAEALR QQMEELKQKE MEATKLKKEQ ENLLRQRWEL
260 270 280 290 300
ERLEEERRQM AALRRKTELG RFLKHQYNAQ LNRRTQEIQE ELEVDGRILQ
310 320 330 340 350
ALLEKEGELQ QVELARREQA RADAAWMKQV IEEQLQLEKA REAELQQLLR
360 370 380 390 400
EEAKEMWEKR EAEWAREQVA RDRLMSEVLT GRQQQILEKI EQNRRAQEET
410 420 430 440 450
LKHREKLIRS LEEGKQLAQR AKEESEELKL ARKQELEAQV AERQGQEWEA
460 470 480 490
ARQEEEEEEE ARQAEEHSNA LLQQEAKTMA EKGYQPKLHG HLRIAWD
Note: No experimental confirmation available.Curated
Length:497
Mass (Da):60,642
Last modified:June 26, 2007 - v2
Checksum:iE6731294EA6E1CD7
GO
Isoform 21 Publication (identifier: Q3TVW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     441-497: AERQGQEWEA...LHGHLRIAWD → GPKGKGGLAGHYRTELGRGP

Show »
Length:460
Mass (Da):55,949
Checksum:iFBE7110A51BE37B9
GO

Sequence cautioni

The sequence AAH47930 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109G → S in BAE35503 (PubMed:16141072).Curated1
Sequence conflicti229 – 232KEME → LWVS in AAH47930 (PubMed:15489334).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052475441 – 497AERQG…RIAWD → GPKGKGGLAGHYRTELGRGP in isoform 2. 2 PublicationsAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020912 mRNA. Translation: BAB32251.1.
AK083027 mRNA. Translation: BAC38735.1.
AK159947 mRNA. Translation: BAE35503.1.
BC043048 mRNA. Translation: AAH43048.1.
BC047930 mRNA. Translation: AAH47930.1. Different initiation.
CCDSiCCDS51624.1. [Q3TVW5-1]
RefSeqiNP_084268.2. NM_029992.2. [Q3TVW5-1]
XP_006530573.1. XM_006530510.3.
UniGeneiMm.239698.

Genome annotation databases

EnsembliENSMUST00000094441; ENSMUSP00000092009; ENSMUSG00000002486. [Q3TVW5-1]
GeneIDi77832.
KEGGimmu:77832.
UCSCiuc008yzx.1. mouse. [Q3TVW5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020912 mRNA. Translation: BAB32251.1.
AK083027 mRNA. Translation: BAC38735.1.
AK159947 mRNA. Translation: BAE35503.1.
BC043048 mRNA. Translation: AAH43048.1.
BC047930 mRNA. Translation: AAH47930.1. Different initiation.
CCDSiCCDS51624.1. [Q3TVW5-1]
RefSeqiNP_084268.2. NM_029992.2. [Q3TVW5-1]
XP_006530573.1. XM_006530510.3.
UniGeneiMm.239698.

3D structure databases

ProteinModelPortaliQ3TVW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TVW5. 1 interactor.
STRINGi10090.ENSMUSP00000092009.

PTM databases

iPTMnetiQ3TVW5.
PhosphoSitePlusiQ3TVW5.

Proteomic databases

MaxQBiQ3TVW5.
PaxDbiQ3TVW5.
PRIDEiQ3TVW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094441; ENSMUSP00000092009; ENSMUSG00000002486. [Q3TVW5-1]
GeneIDi77832.
KEGGimmu:77832.
UCSCiuc008yzx.1. mouse. [Q3TVW5-1]

Organism-specific databases

CTDi84260.
MGIiMGI:1925082. Tchp.

Phylogenomic databases

eggNOGiENOG410IIAX. Eukaryota.
ENOG410ZEPV. LUCA.
GeneTreeiENSGT00730000111170.
HOGENOMiHOG000006708.
HOVERGENiHBG097262.
InParanoidiQ3TVW5.
KOiK16811.
OMAiTLPSYWC.
OrthoDBiEOG091G0FWB.
PhylomeDBiQ3TVW5.
TreeFamiTF329032.

Miscellaneous databases

PROiQ3TVW5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002486.
CleanExiMM_TCHP.
ExpressionAtlasiQ3TVW5. baseline and differential.
GenevisibleiQ3TVW5. MM.

Family and domain databases

InterProiIPR026773. TCHP.
[Graphical view]
PANTHERiPTHR31183:SF2. PTHR31183:SF2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTCHP_MOUSE
AccessioniPrimary (citable) accession number: Q3TVW5
Secondary accession number(s): Q80ZV6, Q8C459, Q9CTP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.