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Q3TUA9

- SG196_MOUSE

UniProt

Q3TUA9 - SG196_MOUSE

Protein

Protein O-mannose kinase

Gene

Pomk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif By similarity.By similarity

    Catalytic activityi

    ATP + N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-O-alpha-D-mannosylprotein = ADP + N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-O-alpha-D-(6-phospho)mannosylprotein.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. carbohydrate kinase activity Source: MGI
    3. phosphotransferase activity, alcohol group as acceptor Source: UniProtKB
    4. protein kinase activity Source: InterPro

    GO - Biological processi

    1. brain development Source: MGI
    2. carbohydrate phosphorylation Source: UniProtKB
    3. learning or memory Source: MGI
    4. neuromuscular process Source: MGI
    5. neuron migration Source: MGI
    6. protein O-linked glycosylation Source: UniProtKB
    7. sensory perception of pain Source: MGI

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-mannose kinase (EC:2.7.1.-)
    Short name:
    POMK
    Alternative name(s):
    Protein kinase-like protein SgK196
    Sugen kinase 196
    Gene namesi
    Name:Pomk
    Synonyms:Sgk196
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1921903. Pomk.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Hydrocephaly: mutant mice exhibit dome-shaped heads of varying severity. Surviving mutant mice display numerous behavioral abnormalities: tremors, and inverted screen testing show 5 of 8 falling off, suggesting impaired motor strength. Impaired sensorimotor gating/attention is suggested by decreased prepulse inhibition, and impaired learning/memory is detected with trace aversive conditioning testing. In testing nociception, decreased paw flinching is observed during both formalin phases, suggesting decreased sensitivity to acute and tonic pain. Histologically, the most obvious changes are hydrocephalus in 4 of 5 and cerebellar dysplasia in all 5. Abnormalities in neuronal migration are evident in other parts of the brain; in the cerebral cortex, there is disorganization of cortical neuron layers, and the dentate gyrus of the hippocampus has a scalloped appearance. The cerebellar dysplasia is characterized by multifocal disorganization of cerebellar cortical neurons, with clusters of external granular neurons being scattered on the surface of the cerebellum and multifocally within the molecular layer of the cerebellum. In some regions, there is incomplete separation of cerebellar folia, and Purkinje cell. neurons were occasionally found in the molecular layer.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Protein O-mannose kinasePRO_0000262998Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ3TUA9.

    PTM databases

    PhosphoSiteiQ3TUA9.

    Expressioni

    Gene expression databases

    BgeeiQ3TUA9.
    CleanExiMM_4930444A02RIK.
    GenevestigatoriQ3TUA9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TUA9.
    SMRiQ3TUA9. Positions 49-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 349307LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4223Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 349270Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43701.
    GeneTreeiENSGT00390000004945.
    HOGENOMiHOG000006624.
    HOVERGENiHBG093945.
    InParanoidiQ3TUA9.
    KOiK17547.
    OMAiKACKSQT.
    OrthoDBiEOG7D59NT.
    PhylomeDBiQ3TUA9.
    TreeFamiTF328472.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3TUA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQQHGTRNG LTHRELPRGV GLLLAMALMN VALYLCLDQL FISPGRSTAD    50
    SRRCPPGYFR MGRMRNCSRW LSCEELRTEV RQLKRVGEGA VKRVFLSEWK 100
    EHKVALSRLT RLEMKEDFLH GLQMLKSLQS EHVVTLVGYC EEDGTILTEY 150
    HPLGSLSNLE ETLNLSKYQD VNTWQHRLQL AMEYVSIINY LHHSPLGTRV 200
    MCDSNDLPKT LSQYLLTSNF SIVANDLDAL PLVDHDSGVL IKCGHRELHG 250
    DFVAPEQLWP YGEDTPFQDD LMPSYNEKVD IWKIPDVSSF LLGHVEGSDM 300
    VRFHLFDIHK ACKSQIPAER PTAQNVLDAY QRVFHSLRDT VMSQTKEML 349
    Length:349
    Mass (Da):39,969
    Last modified:November 28, 2006 - v2
    Checksum:i0E8683A6DBAAE9C3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51H → Y in BAC29393. (PubMed:16141072)Curated
    Sequence conflicti158 – 1581N → D in BAE42167. (PubMed:16141072)Curated
    Sequence conflicti239 – 2391V → I in AAH27296. (PubMed:15489334)Curated
    Sequence conflicti276 – 2761N → T in BAE42167. (PubMed:16141072)Curated
    Sequence conflicti319 – 3191E → K in AAH27296. (PubMed:15489334)Curated
    Sequence conflicti335 – 3351H → Q in BAE36062. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK036348 mRNA. Translation: BAC29393.1.
    AK015374 mRNA. Translation: BAB29817.1.
    AK032677 mRNA. Translation: BAC27984.1.
    AK043620 mRNA. Translation: BAC31598.1.
    AK138952 mRNA. Translation: BAE23832.1.
    AK160873 mRNA. Translation: BAE36062.1.
    AK170994 mRNA. Translation: BAE42167.1.
    BC027296 mRNA. Translation: AAH27296.1.
    CCDSiCCDS22204.1.
    RefSeqiNP_083313.1. NM_029037.4.
    UniGeneiMm.17631.

    Genome annotation databases

    EnsembliENSMUST00000061850; ENSMUSP00000053802; ENSMUSG00000037251.
    GeneIDi74653.
    KEGGimmu:74653.
    UCSCiuc009lhh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK036348 mRNA. Translation: BAC29393.1 .
    AK015374 mRNA. Translation: BAB29817.1 .
    AK032677 mRNA. Translation: BAC27984.1 .
    AK043620 mRNA. Translation: BAC31598.1 .
    AK138952 mRNA. Translation: BAE23832.1 .
    AK160873 mRNA. Translation: BAE36062.1 .
    AK170994 mRNA. Translation: BAE42167.1 .
    BC027296 mRNA. Translation: AAH27296.1 .
    CCDSi CCDS22204.1.
    RefSeqi NP_083313.1. NM_029037.4.
    UniGenei Mm.17631.

    3D structure databases

    ProteinModelPortali Q3TUA9.
    SMRi Q3TUA9. Positions 49-341.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q3TUA9.

    Proteomic databases

    PRIDEi Q3TUA9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061850 ; ENSMUSP00000053802 ; ENSMUSG00000037251 .
    GeneIDi 74653.
    KEGGi mmu:74653.
    UCSCi uc009lhh.1. mouse.

    Organism-specific databases

    CTDi 84197.
    MGIi MGI:1921903. Pomk.

    Phylogenomic databases

    eggNOGi NOG43701.
    GeneTreei ENSGT00390000004945.
    HOGENOMi HOG000006624.
    HOVERGENi HBG093945.
    InParanoidi Q3TUA9.
    KOi K17547.
    OMAi KACKSQT.
    OrthoDBi EOG7D59NT.
    PhylomeDBi Q3TUA9.
    TreeFami TF328472.

    Miscellaneous databases

    NextBioi 341310.
    PROi Q3TUA9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3TUA9.
    CleanExi MM_4930444A02RIK.
    Genevestigatori Q3TUA9.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Aorta, Brain, Brain cortex, Cerebellum, Testis and Vein.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    3. Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiSG196_MOUSE
    AccessioniPrimary (citable) accession number: Q3TUA9
    Secondary accession number(s): Q3TBZ0
    , Q8BZ83, Q8R2S2, Q9D5G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Although related to the Ser/Thr protein kinase family, has no protein kinase activity and acts as a mannose kinase instead.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3