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Protein

Keratin, type II cytoskeletal 2 epidermal

Gene

Krt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei450StutterSequence analysis1

GO - Molecular functioni

  • cytoskeletal protein binding Source: MGI
  • structural molecule activity Source: MGI

GO - Biological processi

  • epidermis development Source: MGI
  • intermediate filament organization Source: MGI
  • keratinization Source: Ensembl
  • keratinocyte activation Source: Ensembl
  • keratinocyte development Source: MGI
  • keratinocyte migration Source: Ensembl
  • keratinocyte proliferation Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 2 epidermal
Alternative name(s):
Cytokeratin-2e
Short name:
CK-2e
Epithelial keratin-2e
Keratin-2 epidermis
Keratin-2e
Short name:
K2e
Type-II keratin Kb2
Gene namesi
Name:Krt2Imported
Synonyms:K2e, Krt2-17Imported, Krt2aBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96699. Krt2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • Golgi apparatus Source: Ensembl
  • keratin filament Source: MGI
  • membrane Source: Ensembl
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Involvement in diseasei

Defects in Krt2 are a cause of ichthyosis bullosa of siemens (IBS). IBS is a rare autosomal dominant disorder displaying a type of epidermolytic hyperkeratosis characterized by extensive blistering from birth. Hyperkeratoses and shedding of the outer layers of the epidermis (molting) are observed in later weeks.

Keywords - Diseasei

Disease mutation, Ichthyosis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002837631 – 707Keratin, type II cytoskeletal 2 epidermalAdd BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22Asymmetric dimethylarginineCombined sources1
Modified residuei25PhosphoserineBy similarity1
Modified residuei28PhosphoserineBy similarity1
Modified residuei52Omega-N-methylarginineCombined sources1
Modified residuei64PhosphoserineBy similarity1
Modified residuei555Omega-N-methylarginineCombined sources1
Modified residuei593Omega-N-methylarginineCombined sources1
Modified residuei607Omega-N-methylarginineBy similarity1
Modified residuei675Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ3TTY5.
PRIDEiQ3TTY5.

PTM databases

iPTMnetiQ3TTY5.
PhosphoSitePlusiQ3TTY5.

Expressioni

Tissue specificityi

Expressed mainly in the middle spinous and granular cells of the epidermis of adult tail, nipple and footsole skin. Also found in ear.3 Publications

Developmental stagei

Induction occurs during the first 2 weeks after birth, being first observed in the epidermis of tail then the footpad and later in the ear.1 Publication

Gene expression databases

BgeeiENSMUSG00000064201.
CleanExiMM_KRT2.
GenevisibleiQ3TTY5. MM.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Associates with KRT10.Curated1 Publication

GO - Molecular functioni

  • cytoskeletal protein binding Source: MGI

Protein-protein interaction databases

BioGridi201033. 7 interactors.
IntActiQ3TTY5. 1 interactor.
MINTiMINT-1867051.
STRINGi10090.ENSMUSP00000023712.

Structurei

3D structure databases

ProteinModelPortaliQ3TTY5.
SMRiQ3TTY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 198HeadSequence analysisAdd BLAST198
Regioni199 – 508RodSequence analysisAdd BLAST310
Regioni199 – 234Coil 1ASequence analysisAdd BLAST36
Regioni235 – 253Linker 1Sequence analysisAdd BLAST19
Regioni254 – 345Coil 1BSequence analysisAdd BLAST92
Regioni346 – 369Linker 12Sequence analysisAdd BLAST24
Regioni370 – 508Coil 2Sequence analysisAdd BLAST139
Regioni509 – 707TailSequence analysisAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi12 – 701Gly-richSequence analysisAdd BLAST690

Sequence similaritiesi

Belongs to the intermediate filament family.Sequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiQ3TTY5.
KOiK07605.
OMAiGEHAIKD.
OrthoDBiEOG091G09KR.
TreeFamiTF317854.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 3 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3TTY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCQISCRSR RGGGGGGGGG FRGFSSGSAV VSGGSRRSNT SFSCISRHGG
60 70 80 90 100
GRGGSGGGGF GSQSLVGLGG YKSISSSVAG NSGGYGGSSF GGSSGFGGGR
110 120 130 140 150
GFGGGQGFGG SGGFGGGSGF GGGQGFGGGS RFGGGSGFGG GGFGGGSFGG
160 170 180 190 200
GRFGGGPGGF GGPGGFPGGG IHEVSVNQSL LQPLDVKVDP EIQNVKSQER
210 220 230 240 250
EQIKTLNNKF ASFIDKVRFL EQQNQVLRTK WELLQQLDVG SRTTNLDPIF
260 270 280 290 300
QAYIGMLKKQ VDRLSAERTS QESELNNMQD LVEDFKKKYE DEINKRTSAE
310 320 330 340 350
NDFVTIKKDV DSCYMDKTEL QARLDILAQE VNFLRTLYDA ELSQLQQDVT
360 370 380 390 400
DTNVILSMDN NRNLDLDSII AEVQNQYEMI AHKSKAESEE LYHSKYEELQ
410 420 430 440 450
VTAVKHGDSL KEIKMEISEL NRTIQRLQGE ISHVKKQCKG VQDSIADAEQ
460 470 480 490 500
RGEHAIKDAR GKLTDLEEAL QQCREDLARL LRDYQELMNT KLSLDVEIAT
510 520 530 540 550
YRKLLEGEEC RMSGDFSDNV SVSITSSTIS SSVASKTGFG SGGQSSGGRG
560 570 580 590 600
SYGGRGGGGG GGSTYGSGGR SSGSRGSGSG SGGGGYSSGG GSRGGSGGGY
610 620 630 640 650
GSGGGSRGGS GGGYGSGGGS GSGGGYSSGG GSRGGSGGGG VSSGGGSRGG
660 670 680 690 700
SSSGGGSRGG SSSGGGGYSS GGGSRGGSSS GGAGSSSEKG GSGSGEGCGS

GVTFSFR
Length:707
Mass (Da):70,923
Last modified:October 11, 2005 - v1
Checksum:i464D375DCBA90EF4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238 – 246DVGSRTTNL → ACRQPHHKP in CAA52788 (PubMed:7508961).Curated9
Sequence conflicti361N → T in CAA52788 (PubMed:7508961).Curated1
Sequence conflicti439K → I in CAA52788 (PubMed:7508961).Curated1
Sequence conflicti451R → H in CAA52788 (PubMed:7508961).Curated1
Sequence conflicti533V → M in CAA52788 (PubMed:7508961).Curated1
Sequence conflicti562Missing in AAI20486 (PubMed:15489334).Curated1
Sequence conflicti564T → S in CAA52788 (PubMed:7508961).Curated1
Sequence conflicti625G → GG in AAI20486 (PubMed:15489334).Curated1
Sequence conflicti641V → A in CAA52788 (PubMed:7508961).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti500T → P in IBS. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74784 mRNA. Translation: CAA52788.1.
AK132476 mRNA. Translation: BAE21186.1.
AK161078 mRNA. Translation: BAE36187.1.
AK161098 mRNA. Translation: BAE36194.1.
BC120485 mRNA. Translation: AAI20486.1.
CCDSiCCDS37220.1.
RefSeqiNP_034798.2. NM_010668.2.
UniGeneiMm.358616.

Genome annotation databases

EnsembliENSMUST00000023712; ENSMUSP00000023712; ENSMUSG00000064201.
GeneIDi16681.
KEGGimmu:16681.
UCSCiuc007xub.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74784 mRNA. Translation: CAA52788.1.
AK132476 mRNA. Translation: BAE21186.1.
AK161078 mRNA. Translation: BAE36187.1.
AK161098 mRNA. Translation: BAE36194.1.
BC120485 mRNA. Translation: AAI20486.1.
CCDSiCCDS37220.1.
RefSeqiNP_034798.2. NM_010668.2.
UniGeneiMm.358616.

3D structure databases

ProteinModelPortaliQ3TTY5.
SMRiQ3TTY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201033. 7 interactors.
IntActiQ3TTY5. 1 interactor.
MINTiMINT-1867051.
STRINGi10090.ENSMUSP00000023712.

PTM databases

iPTMnetiQ3TTY5.
PhosphoSitePlusiQ3TTY5.

Proteomic databases

PaxDbiQ3TTY5.
PRIDEiQ3TTY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023712; ENSMUSP00000023712; ENSMUSG00000064201.
GeneIDi16681.
KEGGimmu:16681.
UCSCiuc007xub.1. mouse.

Organism-specific databases

CTDi3849.
MGIiMGI:96699. Krt2.

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiQ3TTY5.
KOiK07605.
OMAiGEHAIKD.
OrthoDBiEOG091G09KR.
TreeFamiTF317854.

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

PROiQ3TTY5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064201.
CleanExiMM_KRT2.
GenevisibleiQ3TTY5. MM.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 3 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK22E_MOUSE
AccessioniPrimary (citable) accession number: Q3TTY5
Secondary accession number(s): Q0VBW1, Q61869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.