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Q3TTY5

- K22E_MOUSE

UniProt

Q3TTY5 - K22E_MOUSE

Protein

Keratin, type II cytoskeletal 2 epidermal

Gene

Krt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei450 – 4501StutterSequence Analysis

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. keratinization Source: Ensembl
    2. keratinocyte activation Source: Ensembl
    3. keratinocyte migration Source: Ensembl
    4. keratinocyte proliferation Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Keratin, type II cytoskeletal 2 epidermal
    Alternative name(s):
    Cytokeratin-2e
    Short name:
    CK-2e
    Epithelial keratin-2e
    Keratin-2 epidermis
    Keratin-2e
    Short name:
    K2e
    Type-II keratin Kb2
    Gene namesi
    Name:Krt2Imported
    Synonyms:K2e, Krt2-17Imported, Krt2aBy similarity
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:96699. Krt2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: Ensembl
    2. keratin filament Source: InterPro

    Keywords - Cellular componenti

    Intermediate filament, Keratin

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Krt2 are a cause of ichthyosis bullosa of siemens (IBS). IBS is a rare autosomal dominant disorder displaying a type of epidermolytic hyperkeratosis characterized by extensive blistering from birth. Hyperkeratoses and shedding of the outer layers of the epidermis (molting) are observed in later weeks.

    Keywords - Diseasei

    Disease mutation, Ichthyosis

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Keratin, type II cytoskeletal 2 epidermalPRO_0000283763Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei64 – 641PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ3TTY5.
    PaxDbiQ3TTY5.
    PRIDEiQ3TTY5.

    PTM databases

    PhosphoSiteiQ3TTY5.

    Expressioni

    Tissue specificityi

    Expressed mainly in the middle spinous and granular cells of the epidermis of adult tail, nipple and footsole skin. Also found in ear.3 Publications

    Developmental stagei

    Induction occurs during the first 2 weeks after birth, being first observed in the epidermis of tail then the footpad and later in the ear.1 Publication

    Gene expression databases

    BgeeiQ3TTY5.
    CleanExiMM_KRT2.
    GenevestigatoriQ3TTY5.

    Interactioni

    Subunit structurei

    Heterotetramer of two type I and two type II keratins. Associates with KRT10.1 PublicationCurated

    Protein-protein interaction databases

    BioGridi201033. 7 interactions.
    IntActiQ3TTY5. 1 interaction.
    MINTiMINT-1867051.
    STRINGi10090.ENSMUSP00000023712.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TTY5.
    SMRiQ3TTY5. Positions 199-338, 365-507.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 198198HeadSequence AnalysisAdd
    BLAST
    Regioni199 – 508310RodSequence AnalysisAdd
    BLAST
    Regioni199 – 23436Coil 1ASequence AnalysisAdd
    BLAST
    Regioni235 – 25319Linker 1Sequence AnalysisAdd
    BLAST
    Regioni254 – 34592Coil 1BSequence AnalysisAdd
    BLAST
    Regioni346 – 36924Linker 12Sequence AnalysisAdd
    BLAST
    Regioni370 – 508139Coil 2Sequence AnalysisAdd
    BLAST
    Regioni509 – 707199TailSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi12 – 701690Gly-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intermediate filament family.Sequence Analysis

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG146769.
    GeneTreeiENSGT00730000110687.
    HOVERGENiHBG013015.
    InParanoidiQ3TTY5.
    KOiK07605.
    OMAiTNLDPIF.
    OrthoDBiEOG7FV3Q8.
    TreeFamiTF317854.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR003054. Keratin_II.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 1 hit.
    [Graphical view]
    PRINTSiPR01276. TYPE2KERATIN.
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3TTY5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCQISCRSR RGGGGGGGGG FRGFSSGSAV VSGGSRRSNT SFSCISRHGG    50
    GRGGSGGGGF GSQSLVGLGG YKSISSSVAG NSGGYGGSSF GGSSGFGGGR 100
    GFGGGQGFGG SGGFGGGSGF GGGQGFGGGS RFGGGSGFGG GGFGGGSFGG 150
    GRFGGGPGGF GGPGGFPGGG IHEVSVNQSL LQPLDVKVDP EIQNVKSQER 200
    EQIKTLNNKF ASFIDKVRFL EQQNQVLRTK WELLQQLDVG SRTTNLDPIF 250
    QAYIGMLKKQ VDRLSAERTS QESELNNMQD LVEDFKKKYE DEINKRTSAE 300
    NDFVTIKKDV DSCYMDKTEL QARLDILAQE VNFLRTLYDA ELSQLQQDVT 350
    DTNVILSMDN NRNLDLDSII AEVQNQYEMI AHKSKAESEE LYHSKYEELQ 400
    VTAVKHGDSL KEIKMEISEL NRTIQRLQGE ISHVKKQCKG VQDSIADAEQ 450
    RGEHAIKDAR GKLTDLEEAL QQCREDLARL LRDYQELMNT KLSLDVEIAT 500
    YRKLLEGEEC RMSGDFSDNV SVSITSSTIS SSVASKTGFG SGGQSSGGRG 550
    SYGGRGGGGG GGSTYGSGGR SSGSRGSGSG SGGGGYSSGG GSRGGSGGGY 600
    GSGGGSRGGS GGGYGSGGGS GSGGGYSSGG GSRGGSGGGG VSSGGGSRGG 650
    SSSGGGSRGG SSSGGGGYSS GGGSRGGSSS GGAGSSSEKG GSGSGEGCGS 700
    GVTFSFR 707
    Length:707
    Mass (Da):70,923
    Last modified:October 11, 2005 - v1
    Checksum:i464D375DCBA90EF4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2469DVGSRTTNL → ACRQPHHKP in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti361 – 3611N → T in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti439 – 4391K → I in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti451 – 4511R → H in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti533 – 5331V → M in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti562 – 5621Missing in AAI20486. (PubMed:15489334)Curated
    Sequence conflicti564 – 5641T → S in CAA52788. (PubMed:7508961)Curated
    Sequence conflicti625 – 6251G → GG in AAI20486. (PubMed:15489334)Curated
    Sequence conflicti641 – 6411V → A in CAA52788. (PubMed:7508961)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti500 – 5001T → P in IBS. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74784 mRNA. Translation: CAA52788.1.
    AK132476 mRNA. Translation: BAE21186.1.
    AK161078 mRNA. Translation: BAE36187.1.
    AK161098 mRNA. Translation: BAE36194.1.
    BC120485 mRNA. Translation: AAI20486.1.
    CCDSiCCDS37220.1.
    RefSeqiNP_034798.2. NM_010668.2.
    UniGeneiMm.358616.

    Genome annotation databases

    EnsembliENSMUST00000023712; ENSMUSP00000023712; ENSMUSG00000064201.
    GeneIDi16681.
    KEGGimmu:16681.
    UCSCiuc007xub.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74784 mRNA. Translation: CAA52788.1 .
    AK132476 mRNA. Translation: BAE21186.1 .
    AK161078 mRNA. Translation: BAE36187.1 .
    AK161098 mRNA. Translation: BAE36194.1 .
    BC120485 mRNA. Translation: AAI20486.1 .
    CCDSi CCDS37220.1.
    RefSeqi NP_034798.2. NM_010668.2.
    UniGenei Mm.358616.

    3D structure databases

    ProteinModelPortali Q3TTY5.
    SMRi Q3TTY5. Positions 199-338, 365-507.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201033. 7 interactions.
    IntActi Q3TTY5. 1 interaction.
    MINTi MINT-1867051.
    STRINGi 10090.ENSMUSP00000023712.

    PTM databases

    PhosphoSitei Q3TTY5.

    Proteomic databases

    MaxQBi Q3TTY5.
    PaxDbi Q3TTY5.
    PRIDEi Q3TTY5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023712 ; ENSMUSP00000023712 ; ENSMUSG00000064201 .
    GeneIDi 16681.
    KEGGi mmu:16681.
    UCSCi uc007xub.1. mouse.

    Organism-specific databases

    CTDi 3849.
    MGIi MGI:96699. Krt2.

    Phylogenomic databases

    eggNOGi NOG146769.
    GeneTreei ENSGT00730000110687.
    HOVERGENi HBG013015.
    InParanoidi Q3TTY5.
    KOi K07605.
    OMAi TNLDPIF.
    OrthoDBi EOG7FV3Q8.
    TreeFami TF317854.

    Miscellaneous databases

    NextBioi 290429.
    PROi Q3TTY5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3TTY5.
    CleanExi MM_KRT2.
    Genevestigatori Q3TTY5.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR003054. Keratin_II.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 1 hit.
    [Graphical view ]
    PRINTSi PR01276. TYPE2KERATIN.
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An unusual type-II 70-kilodalton keratin protein of mouse epidermis exhibiting postnatal body-site specificity and sensitivity to hyperproliferation."
      Rentrop M., Nischt R., Knapp B., Schweizer J., Winter H.
      Differentiation 34:189-200(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: NMRI1 Publication.
      Tissue: Foot sole tissue1 Publication.
    2. "The large type II 70-kDa keratin of mouse epidermis is the ortholog of human keratin K2e."
      Herzog F., Winter H., Schweizer J.
      J. Invest. Dermatol. 102:165-170(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: NMRIImported.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: SkinImported.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: BrainImported.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 23-36; 210-216; 288-296 AND 483-491, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "Out of balance: consequences of a partial keratin 10 knockout."
      Reichelt J., Bauer C., Porter R., Lane E., Magin V.
      J. Cell Sci. 110:2175-2186(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KRT10.
    7. Cited for: TISSUE SPECIFICITY.
    8. Cited for: VARIANT IBS PRO-500.

    Entry informationi

    Entry nameiK22E_MOUSE
    AccessioniPrimary (citable) accession number: Q3TTY5
    Secondary accession number(s): Q0VBW1, Q61869
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3