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Q3TTY0

- PLB1_MOUSE

UniProt

Q3TTY0 - PLB1_MOUSE

Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.By similarity

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by diisopropyl fluorophosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei404 – 4041NucleophileBy similarity
    Binding sitei478 – 4781Substrate; via amide nitrogenBy similarity
    Binding sitei517 – 5171SubstrateBy similarity
    Active sitei1006 – 10061By similarity
    Active sitei1351 – 13511By similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC
    2. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. positive regulation of acrosome reaction Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.1.5. 3474.
    ReactomeiREACT_198569. Retinoid metabolism and transport.
    REACT_199038. Acyl chain remodelling of PC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B1, membrane-associated
    Short name:
    Phospholipase B
    Alternative name(s):
    Phospholipase B/lipase
    Short name:
    PLB/LIP
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:Plb1
    Synonyms:Plb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1922406. Plb1.

    Subcellular locationi

    Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Present in the intestinal brush border membranes.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 14781455Phospholipase B1, membrane-associatedPRO_0000324385Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi637 – 6371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi830 – 8301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1227 – 12271N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes proteolytic cleavage in the ileum.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ3TTY0.
    PaxDbiQ3TTY0.
    PRIDEiQ3TTY0.

    PTM databases

    PhosphoSiteiQ3TTY0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ3TTY0.
    BgeeiQ3TTY0.
    CleanExiMM_PLB1.
    GenevestigatoriQ3TTY0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TTY0.
    SMRiQ3TTY0. Positions 992-1019.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 14221399ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1444 – 147835CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1423 – 144321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati43 – 3513091Add
    BLAST
    Repeati366 – 7113462Add
    BLAST
    Repeati712 – 10583473Add
    BLAST
    Repeati1068 – 14073404Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni43 – 140713654 X 308-326 AA approximate repeatsAdd
    BLAST
    Regioni1408 – 145043Necessary for membrane localizationBy similarityAdd
    BLAST

    Domaini

    Repeat 2 contains the catalytic domain.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311176.
    GeneTreeiENSGT00530000063883.
    HOGENOMiHOG000115574.
    HOVERGENiHBG108263.
    InParanoidiQ3TTY0.
    KOiK14621.
    OMAiCPDKDPS.
    OrthoDBiEOG7MPRD0.
    PhylomeDBiQ3TTY0.
    TreeFamiTF314942.

    Family and domain databases

    Gene3Di3.40.50.1110. 5 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 3 hits.
    [Graphical view]
    PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3TTY0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELYPGVSPV GLLLLLLLGQ GPSQIHGSSG ENTLAWQSQQ VFWTLKNFPF     50
    PCKPKKLELS VLSESVHSLR PSDIKLVAAI GNPEIPLAPG SGTINMEKPQ 100
    SIKNQPQDVC MGIMTVLSDI IRHFSPSVLM PTCSPGKGTA VHTTAEDLWI 150
    QAKELVRRLK DNPQLDFEKD WKLITVFFSN TSQCHLCPSA QQKSHLMRHM 200
    EMLWGVLDYL HHEVPRAFVN LVDLSEVLAM DLQHQETGFS PAPEVCKCTE 250
    TTTLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQPFF DEIEPPLKRS 300
    SPQDPTTLAL RIWNSMMEPV GQKDGLLNTA ERKTMKCPSE ESPYLFTYKN 350
    SNYQARRLKP ITKLQMKEGS EFTCPDKNPS NSIPTTVHSL RPADIKIIGA 400
    LGDSLTAGNG AGASPWNILD VLTEYRGLSW SVGGDETIKT VTTLPNILRE 450
    FNPSLKGFSV GTGKESTSRA SFNQAVAGAK SDGLAGQARK LVDLMKADKT 500
    INFQEDWKII TVFIGGNDLC ASCSNSTRFS PQNFIDNIKN ALDILHAEVP 550
    RAFVNMAMVM EITPLRELFN EPTVSCPRNI LSRLCPCVLG LGDNSEELSS 600
    LVQRNRDYQK KTEELINSGR YDTRDNFTVV VQPLFENVSM PRTPEGVPDK 650
    SFFAPDCFHF NAKTHARSAI ALWKNMLEPV GHKTRHNNFE IKAPIVCPNQ 700
    ASPFLSTTKN SNLGNGTWMV CEERAPSASP PTSVHTLRPA DIQVVAALGD 750
    SLTAGNGISS QEGNLTDVST QYRGLSYSAG GDKTLENVTT LPNILRKFNG 800
    NLTGYSVGTG DSSSANAFLN QAVPGAKAEN LTSQVRTLVQ KMKSDNRVNF 850
    NRDWKVITVM IGASDLCDFC TDSNHYSAAN FFDHLQNALD ILHKEVPRAL 900
    VNLVDFINPS IIREVFLKNP DKCPVNQSSV LCNCVLTPRK DSYELARLEA 950
    FTKSYQSSML QLVESGRYDT REDFSVVLQP FLLNTKLPVL ENGKPDTSFF 1000
    APDCIHLNQK FHTQLARALW ANMLEPLGKK TDTLDPKGHI SLACPTKDQP 1050
    FLRTFRNSNY KYPTKPAIEN WGSDFLCTEK SPSSQVPTSV HELRPADIKV 1100
    VAAMGDFLTT ATGARPSGYK RLATPWRGLS WSIGGDGKLE THTTLPNILK 1150
    KFNPSITGFS TGTLDNKAGL NVAEEGARAQ DMPAQAKTLV KKMKSTPTIN 1200
    LQEDWKLITL LIGNNDLCLY CENPEDNSTK EYVKYIQQAL DILYEELPRV 1250
    FINVVEVMEL AGLHHVQGGK CAMPLAVQKN CSCLRHSQNL TAMQELKKLN 1300
    WNLQSGISEL SYWHRYMERE DFAVTVQPFF RNTFIPLNER EGLDLTFFSE 1350
    DCFYFSDRGH AEMAIALWNN MLEPVGWKTS SNNFIYNRTK LKCPSPERPF 1400
    LYTLRNSQLL PDKAEEPSNA LYWAVPVAAI GGLAVGILGV MLWRTVKPVQ 1450
    QEEEEEDTLP NTSVTQDAVS EKRLKAGN 1478
    Length:1,478
    Mass (Da):164,541
    Last modified:March 18, 2008 - v2
    Checksum:iB5361AED29BA49E6
    GO
    Isoform 2 (identifier: Q3TTY0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         778-778: S → R
         779-1478: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:778
    Mass (Da):86,140
    Checksum:i2E08238E090D67AA
    GO
    Isoform 3 (identifier: Q3TTY0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         929-929: S → R
         930-1478: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:929
    Mass (Da):102,708
    Checksum:i8AA3E12EB262376A
    GO
    Isoform 4 (identifier: Q3TTY0-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         387-394: VHSLRPAD → GTWLSCSV
         395-1478: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:394
    Mass (Da):44,290
    Checksum:iE6DB3EC17E8F0888
    GO

    Sequence cautioni

    The sequence AAI19078.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei387 – 3948VHSLRPAD → GTWLSCSV in isoform 4. 1 PublicationVSP_032233
    Alternative sequencei395 – 14781084Missing in isoform 4. 1 PublicationVSP_032234Add
    BLAST
    Alternative sequencei778 – 7781S → R in isoform 2. 1 PublicationVSP_032235
    Alternative sequencei779 – 1478700Missing in isoform 2. 1 PublicationVSP_032236Add
    BLAST
    Alternative sequencei929 – 9291S → R in isoform 3. 1 PublicationVSP_032237
    Alternative sequencei930 – 1478549Missing in isoform 3. 1 PublicationVSP_032238Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK015993 mRNA. Translation: BAB30072.1.
    AK161095 mRNA. Translation: BAE36193.1.
    AC102372 Genomic DNA. No translation available.
    AC111030 Genomic DNA. No translation available.
    BC119077 mRNA. Translation: AAI19078.1. Different initiation.
    CCDSiCCDS39060.1. [Q3TTY0-1]
    RefSeqiNP_001074876.1. NM_001081407.1. [Q3TTY0-1]
    NP_084348.1. NM_030072.1.
    XP_006504110.1. XM_006504047.1. [Q3TTY0-1]
    UniGeneiMm.160067.

    Genome annotation databases

    EnsembliENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134. [Q3TTY0-1]
    ENSMUST00000101377; ENSMUSP00000098928; ENSMUSG00000029134. [Q3TTY0-1]
    ENSMUST00000101382; ENSMUSP00000098932; ENSMUSG00000029134. [Q3TTY0-2]
    GeneIDi665270.
    KEGGimmu:665270.
    UCSCiuc008wzk.1. mouse. [Q3TTY0-4]
    uc008wzl.1. mouse. [Q3TTY0-3]
    uc008wzm.1. mouse. [Q3TTY0-2]
    uc008wzn.1. mouse. [Q3TTY0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK015993 mRNA. Translation: BAB30072.1 .
    AK161095 mRNA. Translation: BAE36193.1 .
    AC102372 Genomic DNA. No translation available.
    AC111030 Genomic DNA. No translation available.
    BC119077 mRNA. Translation: AAI19078.1 . Different initiation.
    CCDSi CCDS39060.1. [Q3TTY0-1 ]
    RefSeqi NP_001074876.1. NM_001081407.1. [Q3TTY0-1 ]
    NP_084348.1. NM_030072.1.
    XP_006504110.1. XM_006504047.1. [Q3TTY0-1 ]
    UniGenei Mm.160067.

    3D structure databases

    ProteinModelPortali Q3TTY0.
    SMRi Q3TTY0. Positions 992-1019.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q3TTY0.

    Proteomic databases

    MaxQBi Q3TTY0.
    PaxDbi Q3TTY0.
    PRIDEi Q3TTY0.

    Protocols and materials databases

    DNASUi 665270.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000101376 ; ENSMUSP00000098927 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
    ENSMUST00000101377 ; ENSMUSP00000098928 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
    ENSMUST00000101382 ; ENSMUSP00000098932 ; ENSMUSG00000029134 . [Q3TTY0-2 ]
    GeneIDi 665270.
    KEGGi mmu:665270.
    UCSCi uc008wzk.1. mouse. [Q3TTY0-4 ]
    uc008wzl.1. mouse. [Q3TTY0-3 ]
    uc008wzm.1. mouse. [Q3TTY0-2 ]
    uc008wzn.1. mouse. [Q3TTY0-1 ]

    Organism-specific databases

    CTDi 151056.
    MGIi MGI:1922406. Plb1.

    Phylogenomic databases

    eggNOGi NOG311176.
    GeneTreei ENSGT00530000063883.
    HOGENOMi HOG000115574.
    HOVERGENi HBG108263.
    InParanoidi Q3TTY0.
    KOi K14621.
    OMAi CPDKDPS.
    OrthoDBi EOG7MPRD0.
    PhylomeDBi Q3TTY0.
    TreeFami TF314942.

    Enzyme and pathway databases

    BRENDAi 3.1.1.5. 3474.
    Reactomei REACT_198569. Retinoid metabolism and transport.
    REACT_199038. Acyl chain remodelling of PC.

    Miscellaneous databases

    NextBioi 427077.
    PROi Q3TTY0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q3TTY0.
    Bgeei Q3TTY0.
    CleanExi MM_PLB1.
    Genevestigatori Q3TTY0.

    Family and domain databases

    Gene3Di 3.40.50.1110. 5 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 3 hits.
    [Graphical view ]
    PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
      Strain: C57BL/6J.
      Tissue: Skin and Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-1478 (ISOFORM 3).
      Tissue: Testis.

    Entry informationi

    Entry nameiPLB1_MOUSE
    AccessioniPrimary (citable) accession number: Q3TTY0
    Secondary accession number(s): Q0VEX7, Q9D4Y6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3