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Q3TTY0 (PLB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipase B1, membrane-associated

Short name=Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name=PLB/LIP

Including the following 2 domains:

  1. Phospholipase A2
    EC=3.1.1.4
  2. Lysophospholipase
    EC=3.1.1.5
Gene names
Name:Plb1
Synonyms:Plb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation

Inhibited by diisopropyl fluorophosphate By similarity.

Subcellular location

Apical cell membrane; Single-pass type I membrane protein By similarity. Note: Present in the intestinal brush border membranes By similarity.

Domain

Repeat 2 contains the catalytic domain By similarity.

Post-translational modification

Undergoes proteolytic cleavage in the ileum By similarity.

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.

Sequence caution

The sequence AAI19078.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionlysophospholipase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phospholipase A2 activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3TTY0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3TTY0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     778-778: S → R
     779-1478: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q3TTY0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     929-929: S → R
     930-1478: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q3TTY0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     387-394: VHSLRPAD → GTWLSCSV
     395-1478: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 14781455Phospholipase B1, membrane-associated
PRO_0000324385

Regions

Topological domain24 – 14221399Extracellular Potential
Transmembrane1423 – 144321Helical; Potential
Topological domain1444 – 147835Cytoplasmic Potential
Repeat43 – 3513091
Repeat366 – 7113462
Repeat712 – 10583473
Repeat1068 – 14073404
Region43 – 140713654 X 308-326 AA approximate repeats
Region1408 – 145043Necessary for membrane localization By similarity

Sites

Active site4041Nucleophile By similarity
Active site10061 By similarity
Active site13511 By similarity
Binding site4781Substrate; via amide nitrogen By similarity
Binding site5171Substrate By similarity

Amino acid modifications

Glycosylation5251N-linked (GlcNAc...) Potential
Glycosylation6261N-linked (GlcNAc...) Potential
Glycosylation6371N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential
Glycosylation8301N-linked (GlcNAc...) Potential
Glycosylation12271N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence387 – 3948VHSLRPAD → GTWLSCSV in isoform 4.
VSP_032233
Alternative sequence395 – 14781084Missing in isoform 4.
VSP_032234
Alternative sequence7781S → R in isoform 2.
VSP_032235
Alternative sequence779 – 1478700Missing in isoform 2.
VSP_032236
Alternative sequence9291S → R in isoform 3.
VSP_032237
Alternative sequence930 – 1478549Missing in isoform 3.
VSP_032238

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: B5361AED29BA49E6

FASTA1,478164,541
        10         20         30         40         50         60 
MELYPGVSPV GLLLLLLLGQ GPSQIHGSSG ENTLAWQSQQ VFWTLKNFPF PCKPKKLELS 

        70         80         90        100        110        120 
VLSESVHSLR PSDIKLVAAI GNPEIPLAPG SGTINMEKPQ SIKNQPQDVC MGIMTVLSDI 

       130        140        150        160        170        180 
IRHFSPSVLM PTCSPGKGTA VHTTAEDLWI QAKELVRRLK DNPQLDFEKD WKLITVFFSN 

       190        200        210        220        230        240 
TSQCHLCPSA QQKSHLMRHM EMLWGVLDYL HHEVPRAFVN LVDLSEVLAM DLQHQETGFS 

       250        260        270        280        290        300 
PAPEVCKCTE TTTLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQPFF DEIEPPLKRS 

       310        320        330        340        350        360 
SPQDPTTLAL RIWNSMMEPV GQKDGLLNTA ERKTMKCPSE ESPYLFTYKN SNYQARRLKP 

       370        380        390        400        410        420 
ITKLQMKEGS EFTCPDKNPS NSIPTTVHSL RPADIKIIGA LGDSLTAGNG AGASPWNILD 

       430        440        450        460        470        480 
VLTEYRGLSW SVGGDETIKT VTTLPNILRE FNPSLKGFSV GTGKESTSRA SFNQAVAGAK 

       490        500        510        520        530        540 
SDGLAGQARK LVDLMKADKT INFQEDWKII TVFIGGNDLC ASCSNSTRFS PQNFIDNIKN 

       550        560        570        580        590        600 
ALDILHAEVP RAFVNMAMVM EITPLRELFN EPTVSCPRNI LSRLCPCVLG LGDNSEELSS 

       610        620        630        640        650        660 
LVQRNRDYQK KTEELINSGR YDTRDNFTVV VQPLFENVSM PRTPEGVPDK SFFAPDCFHF 

       670        680        690        700        710        720 
NAKTHARSAI ALWKNMLEPV GHKTRHNNFE IKAPIVCPNQ ASPFLSTTKN SNLGNGTWMV 

       730        740        750        760        770        780 
CEERAPSASP PTSVHTLRPA DIQVVAALGD SLTAGNGISS QEGNLTDVST QYRGLSYSAG 

       790        800        810        820        830        840 
GDKTLENVTT LPNILRKFNG NLTGYSVGTG DSSSANAFLN QAVPGAKAEN LTSQVRTLVQ 

       850        860        870        880        890        900 
KMKSDNRVNF NRDWKVITVM IGASDLCDFC TDSNHYSAAN FFDHLQNALD ILHKEVPRAL 

       910        920        930        940        950        960 
VNLVDFINPS IIREVFLKNP DKCPVNQSSV LCNCVLTPRK DSYELARLEA FTKSYQSSML 

       970        980        990       1000       1010       1020 
QLVESGRYDT REDFSVVLQP FLLNTKLPVL ENGKPDTSFF APDCIHLNQK FHTQLARALW 

      1030       1040       1050       1060       1070       1080 
ANMLEPLGKK TDTLDPKGHI SLACPTKDQP FLRTFRNSNY KYPTKPAIEN WGSDFLCTEK 

      1090       1100       1110       1120       1130       1140 
SPSSQVPTSV HELRPADIKV VAAMGDFLTT ATGARPSGYK RLATPWRGLS WSIGGDGKLE 

      1150       1160       1170       1180       1190       1200 
THTTLPNILK KFNPSITGFS TGTLDNKAGL NVAEEGARAQ DMPAQAKTLV KKMKSTPTIN 

      1210       1220       1230       1240       1250       1260 
LQEDWKLITL LIGNNDLCLY CENPEDNSTK EYVKYIQQAL DILYEELPRV FINVVEVMEL 

      1270       1280       1290       1300       1310       1320 
AGLHHVQGGK CAMPLAVQKN CSCLRHSQNL TAMQELKKLN WNLQSGISEL SYWHRYMERE 

      1330       1340       1350       1360       1370       1380 
DFAVTVQPFF RNTFIPLNER EGLDLTFFSE DCFYFSDRGH AEMAIALWNN MLEPVGWKTS 

      1390       1400       1410       1420       1430       1440 
SNNFIYNRTK LKCPSPERPF LYTLRNSQLL PDKAEEPSNA LYWAVPVAAI GGLAVGILGV 

      1450       1460       1470 
MLWRTVKPVQ QEEEEEDTLP NTSVTQDAVS EKRLKAGN 

« Hide

Isoform 2 [UniParc].

Checksum: 2E08238E090D67AA
Show »

FASTA77886,140
Isoform 3 [UniParc].

Checksum: 8AA3E12EB262376A
Show »

FASTA929102,708
Isoform 4 [UniParc].

Checksum: E6DB3EC17E8F0888
Show »

FASTA39444,290

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
Strain: C57BL/6J.
Tissue: Skin and Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-1478 (ISOFORM 3).
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK015993 mRNA. Translation: BAB30072.1.
AK161095 mRNA. Translation: BAE36193.1.
AC102372 Genomic DNA. No translation available.
AC111030 Genomic DNA. No translation available.
BC119077 mRNA. Translation: AAI19078.1. Different initiation.
RefSeqNP_001074876.1. NM_001081407.1.
NP_084348.1. NM_030072.1.
XP_006504110.1. XM_006504047.1.
UniGeneMm.160067.

3D structure databases

ProteinModelPortalQ3TTY0.
SMRQ3TTY0. Positions 507-554, 650-678, 739-778, 993-1023.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ3TTY0.

Proteomic databases

PaxDbQ3TTY0.
PRIDEQ3TTY0.

Protocols and materials databases

DNASU665270.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101377; ENSMUSP00000098928; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101382; ENSMUSP00000098932; ENSMUSG00000029134. [Q3TTY0-2]
GeneID665270.
KEGGmmu:665270.
UCSCuc008wzk.1. mouse. [Q3TTY0-4]
uc008wzl.1. mouse. [Q3TTY0-3]
uc008wzm.1. mouse. [Q3TTY0-2]
uc008wzn.1. mouse. [Q3TTY0-1]

Organism-specific databases

CTD151056.
MGIMGI:1922406. Plb1.

Phylogenomic databases

eggNOGNOG311176.
GeneTreeENSGT00530000063883.
HOGENOMHOG000115574.
HOVERGENHBG108263.
InParanoidQ3TTY0.
KOK14621.
OMACPDKDPS.
OrthoDBEOG7MPRD0.
PhylomeDBQ3TTY0.
TreeFamTF314942.

Enzyme and pathway databases

BRENDA3.1.1.5. 3474.

Gene expression databases

ArrayExpressQ3TTY0.
BgeeQ3TTY0.
CleanExMM_PLB1.
GenevestigatorQ3TTY0.

Family and domain databases

Gene3D3.40.50.1110. 5 hits.
InterProIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio427077.
PROQ3TTY0.
SOURCESearch...

Entry information

Entry namePLB1_MOUSE
AccessionPrimary (citable) accession number: Q3TTY0
Secondary accession number(s): Q0VEX7, Q9D4Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot