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Q3TTY0

- PLB1_MOUSE

UniProt

Q3TTY0 - PLB1_MOUSE

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Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041NucleophileBy similarity
Binding sitei478 – 4781Substrate; via amide nitrogenBy similarity
Binding sitei517 – 5171SubstrateBy similarity
Active sitei1006 – 10061By similarity
Active sitei1351 – 13511By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. positive regulation of acrosome reaction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5. 3474.
ReactomeiREACT_198569. Retinoid metabolism and transport.
REACT_199038. Acyl chain remodelling of PC.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Plb1
Synonyms:Plb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1922406. Plb1.

Subcellular locationi

Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes.By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 14781455Phospholipase B1, membrane-associatedPRO_0000324385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi637 – 6371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi830 – 8301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1227 – 12271N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3TTY0.
PaxDbiQ3TTY0.
PRIDEiQ3TTY0.

PTM databases

PhosphoSiteiQ3TTY0.

Expressioni

Gene expression databases

BgeeiQ3TTY0.
CleanExiMM_PLB1.
ExpressionAtlasiQ3TTY0. baseline and differential.
GenevestigatoriQ3TTY0.

Structurei

3D structure databases

ProteinModelPortaliQ3TTY0.
SMRiQ3TTY0. Positions 998-1023.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 14221399ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1444 – 147835CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1423 – 144321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati43 – 3513091Add
BLAST
Repeati366 – 7113462Add
BLAST
Repeati712 – 10583473Add
BLAST
Repeati1068 – 14073404Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 140713654 X 308-326 AA approximate repeatsAdd
BLAST
Regioni1408 – 145043Necessary for membrane localizationBy similarityAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
GeneTreeiENSGT00530000063883.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ3TTY0.
KOiK14621.
OMAiCPDKDPS.
OrthoDBiEOG7MPRD0.
PhylomeDBiQ3TTY0.
TreeFamiTF314942.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3TTY0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELYPGVSPV GLLLLLLLGQ GPSQIHGSSG ENTLAWQSQQ VFWTLKNFPF
60 70 80 90 100
PCKPKKLELS VLSESVHSLR PSDIKLVAAI GNPEIPLAPG SGTINMEKPQ
110 120 130 140 150
SIKNQPQDVC MGIMTVLSDI IRHFSPSVLM PTCSPGKGTA VHTTAEDLWI
160 170 180 190 200
QAKELVRRLK DNPQLDFEKD WKLITVFFSN TSQCHLCPSA QQKSHLMRHM
210 220 230 240 250
EMLWGVLDYL HHEVPRAFVN LVDLSEVLAM DLQHQETGFS PAPEVCKCTE
260 270 280 290 300
TTTLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQPFF DEIEPPLKRS
310 320 330 340 350
SPQDPTTLAL RIWNSMMEPV GQKDGLLNTA ERKTMKCPSE ESPYLFTYKN
360 370 380 390 400
SNYQARRLKP ITKLQMKEGS EFTCPDKNPS NSIPTTVHSL RPADIKIIGA
410 420 430 440 450
LGDSLTAGNG AGASPWNILD VLTEYRGLSW SVGGDETIKT VTTLPNILRE
460 470 480 490 500
FNPSLKGFSV GTGKESTSRA SFNQAVAGAK SDGLAGQARK LVDLMKADKT
510 520 530 540 550
INFQEDWKII TVFIGGNDLC ASCSNSTRFS PQNFIDNIKN ALDILHAEVP
560 570 580 590 600
RAFVNMAMVM EITPLRELFN EPTVSCPRNI LSRLCPCVLG LGDNSEELSS
610 620 630 640 650
LVQRNRDYQK KTEELINSGR YDTRDNFTVV VQPLFENVSM PRTPEGVPDK
660 670 680 690 700
SFFAPDCFHF NAKTHARSAI ALWKNMLEPV GHKTRHNNFE IKAPIVCPNQ
710 720 730 740 750
ASPFLSTTKN SNLGNGTWMV CEERAPSASP PTSVHTLRPA DIQVVAALGD
760 770 780 790 800
SLTAGNGISS QEGNLTDVST QYRGLSYSAG GDKTLENVTT LPNILRKFNG
810 820 830 840 850
NLTGYSVGTG DSSSANAFLN QAVPGAKAEN LTSQVRTLVQ KMKSDNRVNF
860 870 880 890 900
NRDWKVITVM IGASDLCDFC TDSNHYSAAN FFDHLQNALD ILHKEVPRAL
910 920 930 940 950
VNLVDFINPS IIREVFLKNP DKCPVNQSSV LCNCVLTPRK DSYELARLEA
960 970 980 990 1000
FTKSYQSSML QLVESGRYDT REDFSVVLQP FLLNTKLPVL ENGKPDTSFF
1010 1020 1030 1040 1050
APDCIHLNQK FHTQLARALW ANMLEPLGKK TDTLDPKGHI SLACPTKDQP
1060 1070 1080 1090 1100
FLRTFRNSNY KYPTKPAIEN WGSDFLCTEK SPSSQVPTSV HELRPADIKV
1110 1120 1130 1140 1150
VAAMGDFLTT ATGARPSGYK RLATPWRGLS WSIGGDGKLE THTTLPNILK
1160 1170 1180 1190 1200
KFNPSITGFS TGTLDNKAGL NVAEEGARAQ DMPAQAKTLV KKMKSTPTIN
1210 1220 1230 1240 1250
LQEDWKLITL LIGNNDLCLY CENPEDNSTK EYVKYIQQAL DILYEELPRV
1260 1270 1280 1290 1300
FINVVEVMEL AGLHHVQGGK CAMPLAVQKN CSCLRHSQNL TAMQELKKLN
1310 1320 1330 1340 1350
WNLQSGISEL SYWHRYMERE DFAVTVQPFF RNTFIPLNER EGLDLTFFSE
1360 1370 1380 1390 1400
DCFYFSDRGH AEMAIALWNN MLEPVGWKTS SNNFIYNRTK LKCPSPERPF
1410 1420 1430 1440 1450
LYTLRNSQLL PDKAEEPSNA LYWAVPVAAI GGLAVGILGV MLWRTVKPVQ
1460 1470
QEEEEEDTLP NTSVTQDAVS EKRLKAGN
Length:1,478
Mass (Da):164,541
Last modified:March 18, 2008 - v2
Checksum:iB5361AED29BA49E6
GO
Isoform 2 (identifier: Q3TTY0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     778-778: S → R
     779-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:778
Mass (Da):86,140
Checksum:i2E08238E090D67AA
GO
Isoform 3 (identifier: Q3TTY0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     929-929: S → R
     930-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:929
Mass (Da):102,708
Checksum:i8AA3E12EB262376A
GO
Isoform 4 (identifier: Q3TTY0-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-394: VHSLRPAD → GTWLSCSV
     395-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:394
Mass (Da):44,290
Checksum:iE6DB3EC17E8F0888
GO

Sequence cautioni

The sequence AAI19078.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei387 – 3948VHSLRPAD → GTWLSCSV in isoform 4. 1 PublicationVSP_032233
Alternative sequencei395 – 14781084Missing in isoform 4. 1 PublicationVSP_032234Add
BLAST
Alternative sequencei778 – 7781S → R in isoform 2. 1 PublicationVSP_032235
Alternative sequencei779 – 1478700Missing in isoform 2. 1 PublicationVSP_032236Add
BLAST
Alternative sequencei929 – 9291S → R in isoform 3. 1 PublicationVSP_032237
Alternative sequencei930 – 1478549Missing in isoform 3. 1 PublicationVSP_032238Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK015993 mRNA. Translation: BAB30072.1.
AK161095 mRNA. Translation: BAE36193.1.
AC102372 Genomic DNA. No translation available.
AC111030 Genomic DNA. No translation available.
BC119077 mRNA. Translation: AAI19078.1. Different initiation.
CCDSiCCDS39060.1. [Q3TTY0-1]
RefSeqiNP_001074876.1. NM_001081407.1. [Q3TTY0-1]
NP_084348.1. NM_030072.1.
XP_006504110.1. XM_006504047.1. [Q3TTY0-1]
UniGeneiMm.160067.

Genome annotation databases

EnsembliENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101377; ENSMUSP00000098928; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101382; ENSMUSP00000098932; ENSMUSG00000029134. [Q3TTY0-2]
GeneIDi665270.
KEGGimmu:665270.
UCSCiuc008wzk.1. mouse. [Q3TTY0-4]
uc008wzl.1. mouse. [Q3TTY0-3]
uc008wzm.1. mouse. [Q3TTY0-2]
uc008wzn.1. mouse. [Q3TTY0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK015993 mRNA. Translation: BAB30072.1 .
AK161095 mRNA. Translation: BAE36193.1 .
AC102372 Genomic DNA. No translation available.
AC111030 Genomic DNA. No translation available.
BC119077 mRNA. Translation: AAI19078.1 . Different initiation.
CCDSi CCDS39060.1. [Q3TTY0-1 ]
RefSeqi NP_001074876.1. NM_001081407.1. [Q3TTY0-1 ]
NP_084348.1. NM_030072.1.
XP_006504110.1. XM_006504047.1. [Q3TTY0-1 ]
UniGenei Mm.160067.

3D structure databases

ProteinModelPortali Q3TTY0.
SMRi Q3TTY0. Positions 998-1023.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q3TTY0.

Proteomic databases

MaxQBi Q3TTY0.
PaxDbi Q3TTY0.
PRIDEi Q3TTY0.

Protocols and materials databases

DNASUi 665270.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000101376 ; ENSMUSP00000098927 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
ENSMUST00000101377 ; ENSMUSP00000098928 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
ENSMUST00000101382 ; ENSMUSP00000098932 ; ENSMUSG00000029134 . [Q3TTY0-2 ]
GeneIDi 665270.
KEGGi mmu:665270.
UCSCi uc008wzk.1. mouse. [Q3TTY0-4 ]
uc008wzl.1. mouse. [Q3TTY0-3 ]
uc008wzm.1. mouse. [Q3TTY0-2 ]
uc008wzn.1. mouse. [Q3TTY0-1 ]

Organism-specific databases

CTDi 151056.
MGIi MGI:1922406. Plb1.

Phylogenomic databases

eggNOGi NOG311176.
GeneTreei ENSGT00530000063883.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi Q3TTY0.
KOi K14621.
OMAi CPDKDPS.
OrthoDBi EOG7MPRD0.
PhylomeDBi Q3TTY0.
TreeFami TF314942.

Enzyme and pathway databases

BRENDAi 3.1.1.5. 3474.
Reactomei REACT_198569. Retinoid metabolism and transport.
REACT_199038. Acyl chain remodelling of PC.

Miscellaneous databases

NextBioi 427077.
PROi Q3TTY0.
SOURCEi Search...

Gene expression databases

Bgeei Q3TTY0.
CleanExi MM_PLB1.
ExpressionAtlasi Q3TTY0. baseline and differential.
Genevestigatori Q3TTY0.

Family and domain databases

Gene3Di 3.40.50.1110. 5 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Strain: C57BL/6J.
    Tissue: Skin and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-1478 (ISOFORM 3).
    Tissue: Testis.

Entry informationi

Entry nameiPLB1_MOUSE
AccessioniPrimary (citable) accession number: Q3TTY0
Secondary accession number(s): Q0VEX7, Q9D4Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3