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Q3TTY0

- PLB1_MOUSE

UniProt

Q3TTY0 - PLB1_MOUSE

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Protein
Phospholipase B1, membrane-associated
Gene
Plb1, Plb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041Nucleophile By similarity
Binding sitei478 – 4781Substrate; via amide nitrogen By similarity
Binding sitei517 – 5171Substrate By similarity
Active sitei1006 – 10061 By similarity
Active sitei1351 – 13511 By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5. 3474.
ReactomeiREACT_198569. Retinoid metabolism and transport.
REACT_199038. Acyl chain remodelling of PC.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Plb1
Synonyms:Plb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1922406. Plb1.

Subcellular locationi

Apical cell membrane; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 14221399Extracellular Reviewed prediction
Add
BLAST
Transmembranei1423 – 144321Helical; Reviewed prediction
Add
BLAST
Topological domaini1444 – 147835Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 14781455Phospholipase B1, membrane-associated
PRO_0000324385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi525 – 5251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi626 – 6261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi637 – 6371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi801 – 8011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi830 – 8301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1227 – 12271N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3TTY0.
PaxDbiQ3TTY0.
PRIDEiQ3TTY0.

PTM databases

PhosphoSiteiQ3TTY0.

Expressioni

Gene expression databases

ArrayExpressiQ3TTY0.
BgeeiQ3TTY0.
CleanExiMM_PLB1.
GenevestigatoriQ3TTY0.

Structurei

3D structure databases

ProteinModelPortaliQ3TTY0.
SMRiQ3TTY0. Positions 992-1019.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati43 – 3513091
Add
BLAST
Repeati366 – 7113462
Add
BLAST
Repeati712 – 10583473
Add
BLAST
Repeati1068 – 14073404
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 140713654 X 308-326 AA approximate repeats
Add
BLAST
Regioni1408 – 145043Necessary for membrane localization By similarity
Add
BLAST

Domaini

Repeat 2 contains the catalytic domain By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
GeneTreeiENSGT00530000063883.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ3TTY0.
KOiK14621.
OMAiCPDKDPS.
OrthoDBiEOG7MPRD0.
PhylomeDBiQ3TTY0.
TreeFamiTF314942.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3TTY0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MELYPGVSPV GLLLLLLLGQ GPSQIHGSSG ENTLAWQSQQ VFWTLKNFPF     50
PCKPKKLELS VLSESVHSLR PSDIKLVAAI GNPEIPLAPG SGTINMEKPQ 100
SIKNQPQDVC MGIMTVLSDI IRHFSPSVLM PTCSPGKGTA VHTTAEDLWI 150
QAKELVRRLK DNPQLDFEKD WKLITVFFSN TSQCHLCPSA QQKSHLMRHM 200
EMLWGVLDYL HHEVPRAFVN LVDLSEVLAM DLQHQETGFS PAPEVCKCTE 250
TTTLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQPFF DEIEPPLKRS 300
SPQDPTTLAL RIWNSMMEPV GQKDGLLNTA ERKTMKCPSE ESPYLFTYKN 350
SNYQARRLKP ITKLQMKEGS EFTCPDKNPS NSIPTTVHSL RPADIKIIGA 400
LGDSLTAGNG AGASPWNILD VLTEYRGLSW SVGGDETIKT VTTLPNILRE 450
FNPSLKGFSV GTGKESTSRA SFNQAVAGAK SDGLAGQARK LVDLMKADKT 500
INFQEDWKII TVFIGGNDLC ASCSNSTRFS PQNFIDNIKN ALDILHAEVP 550
RAFVNMAMVM EITPLRELFN EPTVSCPRNI LSRLCPCVLG LGDNSEELSS 600
LVQRNRDYQK KTEELINSGR YDTRDNFTVV VQPLFENVSM PRTPEGVPDK 650
SFFAPDCFHF NAKTHARSAI ALWKNMLEPV GHKTRHNNFE IKAPIVCPNQ 700
ASPFLSTTKN SNLGNGTWMV CEERAPSASP PTSVHTLRPA DIQVVAALGD 750
SLTAGNGISS QEGNLTDVST QYRGLSYSAG GDKTLENVTT LPNILRKFNG 800
NLTGYSVGTG DSSSANAFLN QAVPGAKAEN LTSQVRTLVQ KMKSDNRVNF 850
NRDWKVITVM IGASDLCDFC TDSNHYSAAN FFDHLQNALD ILHKEVPRAL 900
VNLVDFINPS IIREVFLKNP DKCPVNQSSV LCNCVLTPRK DSYELARLEA 950
FTKSYQSSML QLVESGRYDT REDFSVVLQP FLLNTKLPVL ENGKPDTSFF 1000
APDCIHLNQK FHTQLARALW ANMLEPLGKK TDTLDPKGHI SLACPTKDQP 1050
FLRTFRNSNY KYPTKPAIEN WGSDFLCTEK SPSSQVPTSV HELRPADIKV 1100
VAAMGDFLTT ATGARPSGYK RLATPWRGLS WSIGGDGKLE THTTLPNILK 1150
KFNPSITGFS TGTLDNKAGL NVAEEGARAQ DMPAQAKTLV KKMKSTPTIN 1200
LQEDWKLITL LIGNNDLCLY CENPEDNSTK EYVKYIQQAL DILYEELPRV 1250
FINVVEVMEL AGLHHVQGGK CAMPLAVQKN CSCLRHSQNL TAMQELKKLN 1300
WNLQSGISEL SYWHRYMERE DFAVTVQPFF RNTFIPLNER EGLDLTFFSE 1350
DCFYFSDRGH AEMAIALWNN MLEPVGWKTS SNNFIYNRTK LKCPSPERPF 1400
LYTLRNSQLL PDKAEEPSNA LYWAVPVAAI GGLAVGILGV MLWRTVKPVQ 1450
QEEEEEDTLP NTSVTQDAVS EKRLKAGN 1478
Length:1,478
Mass (Da):164,541
Last modified:March 18, 2008 - v2
Checksum:iB5361AED29BA49E6
GO
Isoform 2 (identifier: Q3TTY0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     778-778: S → R
     779-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:778
Mass (Da):86,140
Checksum:i2E08238E090D67AA
GO
Isoform 3 (identifier: Q3TTY0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     929-929: S → R
     930-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:929
Mass (Da):102,708
Checksum:i8AA3E12EB262376A
GO
Isoform 4 (identifier: Q3TTY0-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-394: VHSLRPAD → GTWLSCSV
     395-1478: Missing.

Note: No experimental confirmation available.

Show »
Length:394
Mass (Da):44,290
Checksum:iE6DB3EC17E8F0888
GO

Sequence cautioni

The sequence AAI19078.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei387 – 3948VHSLRPAD → GTWLSCSV in isoform 4.
VSP_032233
Alternative sequencei395 – 14781084Missing in isoform 4.
VSP_032234Add
BLAST
Alternative sequencei778 – 7781S → R in isoform 2.
VSP_032235
Alternative sequencei779 – 1478700Missing in isoform 2.
VSP_032236Add
BLAST
Alternative sequencei929 – 9291S → R in isoform 3.
VSP_032237
Alternative sequencei930 – 1478549Missing in isoform 3.
VSP_032238Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK015993 mRNA. Translation: BAB30072.1.
AK161095 mRNA. Translation: BAE36193.1.
AC102372 Genomic DNA. No translation available.
AC111030 Genomic DNA. No translation available.
BC119077 mRNA. Translation: AAI19078.1. Different initiation.
CCDSiCCDS39060.1. [Q3TTY0-1]
RefSeqiNP_001074876.1. NM_001081407.1. [Q3TTY0-1]
NP_084348.1. NM_030072.1.
XP_006504110.1. XM_006504047.1. [Q3TTY0-1]
UniGeneiMm.160067.

Genome annotation databases

EnsembliENSMUST00000101376; ENSMUSP00000098927; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101377; ENSMUSP00000098928; ENSMUSG00000029134. [Q3TTY0-1]
ENSMUST00000101382; ENSMUSP00000098932; ENSMUSG00000029134. [Q3TTY0-2]
GeneIDi665270.
KEGGimmu:665270.
UCSCiuc008wzk.1. mouse. [Q3TTY0-4]
uc008wzl.1. mouse. [Q3TTY0-3]
uc008wzm.1. mouse. [Q3TTY0-2]
uc008wzn.1. mouse. [Q3TTY0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK015993 mRNA. Translation: BAB30072.1 .
AK161095 mRNA. Translation: BAE36193.1 .
AC102372 Genomic DNA. No translation available.
AC111030 Genomic DNA. No translation available.
BC119077 mRNA. Translation: AAI19078.1 . Different initiation.
CCDSi CCDS39060.1. [Q3TTY0-1 ]
RefSeqi NP_001074876.1. NM_001081407.1. [Q3TTY0-1 ]
NP_084348.1. NM_030072.1.
XP_006504110.1. XM_006504047.1. [Q3TTY0-1 ]
UniGenei Mm.160067.

3D structure databases

ProteinModelPortali Q3TTY0.
SMRi Q3TTY0. Positions 992-1019.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q3TTY0.

Proteomic databases

MaxQBi Q3TTY0.
PaxDbi Q3TTY0.
PRIDEi Q3TTY0.

Protocols and materials databases

DNASUi 665270.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000101376 ; ENSMUSP00000098927 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
ENSMUST00000101377 ; ENSMUSP00000098928 ; ENSMUSG00000029134 . [Q3TTY0-1 ]
ENSMUST00000101382 ; ENSMUSP00000098932 ; ENSMUSG00000029134 . [Q3TTY0-2 ]
GeneIDi 665270.
KEGGi mmu:665270.
UCSCi uc008wzk.1. mouse. [Q3TTY0-4 ]
uc008wzl.1. mouse. [Q3TTY0-3 ]
uc008wzm.1. mouse. [Q3TTY0-2 ]
uc008wzn.1. mouse. [Q3TTY0-1 ]

Organism-specific databases

CTDi 151056.
MGIi MGI:1922406. Plb1.

Phylogenomic databases

eggNOGi NOG311176.
GeneTreei ENSGT00530000063883.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi Q3TTY0.
KOi K14621.
OMAi CPDKDPS.
OrthoDBi EOG7MPRD0.
PhylomeDBi Q3TTY0.
TreeFami TF314942.

Enzyme and pathway databases

BRENDAi 3.1.1.5. 3474.
Reactomei REACT_198569. Retinoid metabolism and transport.
REACT_199038. Acyl chain remodelling of PC.

Miscellaneous databases

NextBioi 427077.
PROi Q3TTY0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q3TTY0.
Bgeei Q3TTY0.
CleanExi MM_PLB1.
Genevestigatori Q3TTY0.

Family and domain databases

Gene3Di 3.40.50.1110. 5 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Strain: C57BL/6J.
    Tissue: Skin and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-1478 (ISOFORM 3).
    Tissue: Testis.

Entry informationi

Entry nameiPLB1_MOUSE
AccessioniPrimary (citable) accession number: Q3TTY0
Secondary accession number(s): Q0VEX7, Q9D4Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: September 3, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi