Reviewed, UniProtKB/Swiss-Prot Q3TRM8 (HXK3_MOUSE)

Names and origin

Protein names

Recommended name:
    Hexokinase-3
    EC=2.7.1.1
Alternative name(s):
    Hexokinase type III
      Short name=HK III

Gene names

Name:

Hk3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation	Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P By similarity.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer By similarity.
Domain	The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
Miscellaneous	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 922	922	Hexokinase-3		PRO_0000197591
Regions
	Nucleotide binding	93 – 98	6	ATP Potential
	Nucleotide binding	540 – 545	6	ATP Potential
	Region	1 – 486	486	Regulatory
	Region	160 – 186	27	Glucose-binding Potential
	Region	487 – 922	436	Catalytic
	Region	602 – 628	27	Glucose-binding Potential
Amino acid modifications
	Modified residue	732	1	Phosphothreonine Ref.2
Experimental info
	Sequence conflict	203	1	A → S in BAE37000. Ref.1
	Sequence conflict	384	1	Y → C in BAE29220. Ref.1
	Sequence conflict	439	1	K → E in BAE29220. Ref.1
	Sequence conflict	442	1	V → I in BAE29007. Ref.1
	Sequence conflict	509	1	G → E in BAE29007. Ref.1
	Sequence conflict	860	1	T → A in BAE29007. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3TRM8-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 38F4E024630C45E5	FASTA	922	100,101
	10 20 30 40 50 60 MATIGPSGLH PGERASVCPH EGVPRPSGSL ELECLQQFKV TRTQLQQIQA SLLCSMEQAL 70 80 90 100 110 120 KGQDSPAPSV RMLPTYVRST PHGTEQGDFL VLELGATGAS LRVLWVTLTG TKECRVEPRS 130 140 150 160 170 180 REFVIPQEVI LGAGQQLFDF AARCLSEFLD AYPVENQGLK LGFNFSFPCH QTGLDRSTLI 190 200 210 220 230 240 SWTKGFRCSG VEGQDVVQLL RDAIQRQGTY RIDVVAMVND TVGTMMGCEL GTRPCEVGLI 250 260 270 280 290 300 VDTGTNACYM EEARHVAALD EDRGRTCVSI EWGSFYDEDA LGPVLTTFDS ALDRESLTPG 310 320 330 340 350 360 AQRFEKMIGG LYLGELVRLV LVHLTQHGVL FDGCASPALL SQGCILLDHV AEMEDTATGT 370 380 390 400 410 420 ARVHTILQDL GLSPRASDAE LVQYVCVAVC TRAAQLCAAA LAAVLSRLQH SREQQTLQVA 430 440 450 460 470 480 VATGGRVFER HPRFLRILKE TVTLLAPNCD VSFIPSVDGG GRGVAMVTAV AARLAAHRRI 490 500 510 520 530 540 LEETLAPFQL TLEQMTVVQA QMREAMIRGL QGEASSLRML PTYVRATPDG SERGDFLALD 550 560 570 580 590 600 LGGTNFRVLL VRVAEGSVQI INQVYSIPEC RAQGSGQKLF DHIVDCIVDF QKRQGLSGQS 610 620 630 640 650 660 LPLGFTFSFP CKQLGLDQGI LLNWTKGFNA SGCEGQDVVY LLREAIRRRQ AVELNVVAIV 670 680 690 700 710 720 NDTVGTMMSC GYDDPRCEMG LIVGTGTNAC YMEELRNVAS VPGDSGLMCI NMEWGAFGDD 730 740 750 760 770 780 GSLGTLSTRF DTSVDQASIN PGKQRFEKMI SGMYLGEIVR HILLHLTNLG VLFRGQKTQC 790 800 810 820 830 840 LQARDIFKTK FLSEIESDSL ALRQVRAILE DLGLTLTSDD ALMVLEVCQA VSRRAAQLCG 850 860 870 880 890 900 AGVAAVVEKI RENRGLQELT VSVGVDGTLY KLHPHFSKLV SATVRKLAPQ CTVTFLQSED 910 920 GSGKGAALVT AVACRLTQMA HV

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Bone and Bone marrow.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-732, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK149651 mRNA. Translation: BAE29007.1. AK149992 mRNA. Translation: BAE29220.1. AK154603 mRNA. Translation: BAE32706.1. AK162637 mRNA. Translation: BAE37000.1. AK171580 mRNA. Translation: BAE42538.1.
IPI	IPI00751595.
RefSeq	NP_001028417.1.
UniGene	Mm.267479
3D structure databases
SMR	Q3TRM8. Positions 33-916.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3TRM8.
PTM databases
PhosphoSite	Q3TRM8.
Proteomic databases
PRIDE	Q3TRM8.
Genome annotation databases
Ensembl	ENSMUST00000052949; ENSMUSP00000051215; ENSMUSG00000025877; Mus musculus. [Genome view]
GeneID	212032.
KEGG	mmu:212032.
UCSC	uc007qpr.1. mouse.
Organism-specific databases
CTD	212032.
MGI	MGI:2670962. Hk3.
Phylogenomic databases
HOGENOM	HBG446386.
HOVERGEN	Q3TRM8.
InParanoid	Q3TRM8.
OMA	TLISWTK.
OrthoDB	EOG989760.
PhylomeDB	Q3TRM8.
Enzyme and pathway databases
BRENDA	2.7.1.1. 244.
Gene expression databases
ArrayExpress	Q3TRM8.
Bgee	Q3TRM8.
CleanEx	MM_HK3.
Genevestigator	Q3TRM8.
GermOnline	ENSMUSG00000025877. Mus musculus.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 2 hits. PF03727. Hexokinase_2. 2 hits. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	373427.
SOURCE	Search...

Entry information

Entry name

HXK3_MOUSE

Accession

Primary (citable) accession number: Q3TRM8
Secondary accession number(s): Q3TAX6, Q3UDP1, Q3UEA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)