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Protein

Hexokinase-3

Gene

Hk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.By similarity

Pathway: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 986ATPSequence Analysis
Nucleotide bindingi540 – 5456ATPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_297294. Glucose transport.
REACT_314687. Glycolysis.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:Hk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2670962. Hk3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • mitochondrion Source: MGI
  • protein complex Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Hexokinase-3PRO_0000197591Add
BLAST

Proteomic databases

MaxQBiQ3TRM8.
PaxDbiQ3TRM8.
PRIDEiQ3TRM8.

PTM databases

PhosphoSiteiQ3TRM8.

Expressioni

Gene expression databases

BgeeiQ3TRM8.
CleanExiMM_HK3.
ExpressionAtlasiQ3TRM8. baseline and differential.
GenevisibleiQ3TRM8. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051215.

Structurei

3D structure databases

ProteinModelPortaliQ3TRM8.
SMRiQ3TRM8. Positions 35-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 469445Hexokinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini475 – 911437Hexokinase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 486486RegulatoryAdd
BLAST
Regioni82 – 218137Hexokinase small subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni160 – 18627Glucose-bindingSequence AnalysisAdd
BLAST
Regioni219 – 458240Hexokinase large subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni487 – 922436CatalyticAdd
BLAST
Regioni529 – 660132Hexokinase small subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni602 – 62827Glucose-bindingSequence AnalysisAdd
BLAST
Regioni661 – 900240Hexokinase large subdomain 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiQ3TRM8.
KOiK00844.
OMAiHGTEQGD.
OrthoDBiEOG7S21X5.
PhylomeDBiQ3TRM8.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3TRM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIGPSGLH PGERASVCPH EGVPRPSGSL ELECLQQFKV TRTQLQQIQA
60 70 80 90 100
SLLCSMEQAL KGQDSPAPSV RMLPTYVRST PHGTEQGDFL VLELGATGAS
110 120 130 140 150
LRVLWVTLTG TKECRVEPRS REFVIPQEVI LGAGQQLFDF AARCLSEFLD
160 170 180 190 200
AYPVENQGLK LGFNFSFPCH QTGLDRSTLI SWTKGFRCSG VEGQDVVQLL
210 220 230 240 250
RDAIQRQGTY RIDVVAMVND TVGTMMGCEL GTRPCEVGLI VDTGTNACYM
260 270 280 290 300
EEARHVAALD EDRGRTCVSI EWGSFYDEDA LGPVLTTFDS ALDRESLTPG
310 320 330 340 350
AQRFEKMIGG LYLGELVRLV LVHLTQHGVL FDGCASPALL SQGCILLDHV
360 370 380 390 400
AEMEDTATGT ARVHTILQDL GLSPRASDAE LVQYVCVAVC TRAAQLCAAA
410 420 430 440 450
LAAVLSRLQH SREQQTLQVA VATGGRVFER HPRFLRILKE TVTLLAPNCD
460 470 480 490 500
VSFIPSVDGG GRGVAMVTAV AARLAAHRRI LEETLAPFQL TLEQMTVVQA
510 520 530 540 550
QMREAMIRGL QGEASSLRML PTYVRATPDG SERGDFLALD LGGTNFRVLL
560 570 580 590 600
VRVAEGSVQI INQVYSIPEC RAQGSGQKLF DHIVDCIVDF QKRQGLSGQS
610 620 630 640 650
LPLGFTFSFP CKQLGLDQGI LLNWTKGFNA SGCEGQDVVY LLREAIRRRQ
660 670 680 690 700
AVELNVVAIV NDTVGTMMSC GYDDPRCEMG LIVGTGTNAC YMEELRNVAS
710 720 730 740 750
VPGDSGLMCI NMEWGAFGDD GSLGTLSTRF DTSVDQASIN PGKQRFEKMI
760 770 780 790 800
SGMYLGEIVR HILLHLTNLG VLFRGQKTQC LQARDIFKTK FLSEIESDSL
810 820 830 840 850
ALRQVRAILE DLGLTLTSDD ALMVLEVCQA VSRRAAQLCG AGVAAVVEKI
860 870 880 890 900
RENRGLQELT VSVGVDGTLY KLHPHFSKLV SATVRKLAPQ CTVTFLQSED
910 920
GSGKGAALVT AVACRLTQMA HV
Length:922
Mass (Da):100,101
Last modified:December 6, 2005 - v2
Checksum:i38F4E024630C45E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031A → S in BAE37000 (PubMed:16141072).Curated
Sequence conflicti384 – 3841Y → C in BAE29220 (PubMed:16141072).Curated
Sequence conflicti439 – 4391K → E in BAE29220 (PubMed:16141072).Curated
Sequence conflicti442 – 4421V → I in BAE29007 (PubMed:16141072).Curated
Sequence conflicti509 – 5091G → E in BAE29007 (PubMed:16141072).Curated
Sequence conflicti860 – 8601T → A in BAE29007 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149651 mRNA. Translation: BAE29007.1.
AK149992 mRNA. Translation: BAE29220.1.
AK154603 mRNA. Translation: BAE32706.1.
AK162637 mRNA. Translation: BAE37000.1.
AK171580 mRNA. Translation: BAE42538.1.
CCDSiCCDS26538.1.
RefSeqiNP_001028417.1. NM_001033245.4.
NP_001193319.1. NM_001206390.1.
UniGeneiMm.267479.

Genome annotation databases

EnsembliENSMUST00000052949; ENSMUSP00000051215; ENSMUSG00000025877.
ENSMUST00000126234; ENSMUSP00000123233; ENSMUSG00000025877.
GeneIDi212032.
KEGGimmu:212032.
UCSCiuc007qpr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149651 mRNA. Translation: BAE29007.1.
AK149992 mRNA. Translation: BAE29220.1.
AK154603 mRNA. Translation: BAE32706.1.
AK162637 mRNA. Translation: BAE37000.1.
AK171580 mRNA. Translation: BAE42538.1.
CCDSiCCDS26538.1.
RefSeqiNP_001028417.1. NM_001033245.4.
NP_001193319.1. NM_001206390.1.
UniGeneiMm.267479.

3D structure databases

ProteinModelPortaliQ3TRM8.
SMRiQ3TRM8. Positions 35-918.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051215.

PTM databases

PhosphoSiteiQ3TRM8.

Proteomic databases

MaxQBiQ3TRM8.
PaxDbiQ3TRM8.
PRIDEiQ3TRM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052949; ENSMUSP00000051215; ENSMUSG00000025877.
ENSMUST00000126234; ENSMUSP00000123233; ENSMUSG00000025877.
GeneIDi212032.
KEGGimmu:212032.
UCSCiuc007qpr.2. mouse.

Organism-specific databases

CTDi3101.
MGIiMGI:2670962. Hk3.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiQ3TRM8.
KOiK00844.
OMAiHGTEQGD.
OrthoDBiEOG7S21X5.
PhylomeDBiQ3TRM8.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
ReactomeiREACT_297294. Glucose transport.
REACT_314687. Glycolysis.

Miscellaneous databases

NextBioi373427.
PROiQ3TRM8.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TRM8.
CleanExiMM_HK3.
ExpressionAtlasiQ3TRM8. baseline and differential.
GenevisibleiQ3TRM8. MM.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone and Bone marrow.

Entry informationi

Entry nameiHXK3_MOUSE
AccessioniPrimary (citable) accession number: Q3TRM8
Secondary accession number(s): Q3TAX6, Q3UDP1, Q3UEA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.