Reviewed, UniProtKB/Swiss-Prot Q3TMX7 (QSOX2_MOUSE)

Names and origin

Protein names

Recommended name:
    Sulfhydryl oxidase 2
    EC=1.8.3.2
Alternative name(s):
    Quiescin Q6-like protein 1

Gene names

Name:	Qsox2
Synonyms:	Qscn6l1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins By similarity.
Catalytic activity	4 R'C(R)SH + O2 = 2 R'C(R)S-S(R)CR' + 2 H2O.
Cofactor	Binds 1 FAD per subunit Potential.
Subcellular location	Membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the quiescin-sulfhydryl oxidase (QSOX) family. Contains 1 ERV/ALR sulfhydryl oxidase domain. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3TMX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3TMX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-666: EWEAQGREQE...LCVVLYVASS → SVLRARPWLG...WSVSLRIGPI
     667-692: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q3TMX7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-165: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Potential
	Chain	39 – 692	654	Sulfhydryl oxidase 2		PRO_0000249539
Regions
	Transmembrane	656 – 676	21	Potential
	Domain	54 – 172	119	Thioredoxin
	Domain	415 – 524	110	ERV/ALR sulfhydryl oxidase
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 165	165	Missing in isoform 3.		VSP_020500
	Alternative sequence	574 – 666	93	EWEAQ…YVASS → SVLRARPWLGQMARLSHVNL LPHFPVEEVSSLKPGVLCLK TNKPRSSLGVSKDEHSWSVS LRIGPI in isoform 2.		VSP_020501
	Alternative sequence	667 – 692	26	Missing in isoform 2.		VSP_020502

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 3BDC7058817484A1	FASTA	692	77,775
	10 20 30 40 50 60 MAAARAVARD PGAYARQPPS LRAARLPRLL FLLAVVAAVG PREGGGARLY REGSDAVWLL 70 80 90 100 110 120 DSGSVRSATG NSSAAWLVQF HSSWCGHCIG YAPTWRALAA DVRDWAAAIR VAALDCAEEK 130 140 150 160 170 180 NQDVCRTYDI HFYPTFRYFK AFTKEFTTGE NFKGPDRELR TVRQTMIDFL QNHTEGTWPP 190 200 210 220 230 240 ACPPLDPIQS SDILSFMDSH SGQYHAIVFE SNGSYVGREV ILDLIPYENI MVSRALDTDK 250 260 270 280 290 300 AFLGTLGITS VPSCYLIYPN GSHGLVNVAK PLRSFFSSHL KSLPDVRKKS LFLPEKSNKE 310 320 330 340 350 360 EKSEVVVWKE FDRAKLYTAD LESGLHYLLR VELAAHRSLA GAQLKTFRDF VTVVAKLFPG 370 380 390 400 410 420 RPAVKKLLET LQEWLANLPL DKIPYNAILD LVNNKMQISG IFLTSHVKWV GCQGSRLELR 430 440 450 460 470 480 GYPCSLWKLF HTLTVQASTH PEALAGTGFE GHPQAVLQAI RRYIRTFFGC KECGEHFEEM 490 500 510 520 530 540 AKESMDSVKT PDQAVLWLWR KHNMVNSRLA GHLSEDPKFP KVPWPTPDLC PACHEEIKGL 550 560 570 580 590 600 DSWNEGQVLL FLKQHYSRDN LVDAYSVDQG SPGEWEAQGR EQEEGKGLNP SGKSWRHHDT 610 620 630 640 650 660 GSLRPPHILG PRTDLSKSLH HRLDLRLQSP QGPQALKEAK AVVPFLGVGF SSLDMSLCVV 670 680 690 LYVASSLFLM IMYFFFRVRS KRWKVRLYHP AV
	Isoform 2. Checksum: B61C2ECE6ABC6477	FASTA	639	71,477
	10 20 30 40 50 60 MAAARAVARD PGAYARQPPS LRAARLPRLL FLLAVVAAVG PREGGGARLY REGSDAVWLL 70 80 90 100 110 120 DSGSVRSATG NSSAAWLVQF HSSWCGHCIG YAPTWRALAA DVRDWAAAIR VAALDCAEEK 130 140 150 160 170 180 NQDVCRTYDI HFYPTFRYFK AFTKEFTTGE NFKGPDRELR TVRQTMIDFL QNHTEGTWPP 190 200 210 220 230 240 ACPPLDPIQS SDILSFMDSH SGQYHAIVFE SNGSYVGREV ILDLIPYENI MVSRALDTDK 250 260 270 280 290 300 AFLGTLGITS VPSCYLIYPN GSHGLVNVAK PLRSFFSSHL KSLPDVRKKS LFLPEKSNKE 310 320 330 340 350 360 EKSEVVVWKE FDRAKLYTAD LESGLHYLLR VELAAHRSLA GAQLKTFRDF VTVVAKLFPG 370 380 390 400 410 420 RPAVKKLLET LQEWLANLPL DKIPYNAILD LVNNKMQISG IFLTSHVKWV GCQGSRLELR 430 440 450 460 470 480 GYPCSLWKLF HTLTVQASTH PEALAGTGFE GHPQAVLQAI RRYIRTFFGC KECGEHFEEM 490 500 510 520 530 540 AKESMDSVKT PDQAVLWLWR KHNMVNSRLA GHLSEDPKFP KVPWPTPDLC PACHEEIKGL 550 560 570 580 590 600 DSWNEGQVLL FLKQHYSRDN LVDAYSVDQG SPGSVLRARP WLGQMARLSH VNLLPHFPVE 610 620 630 EVSSLKPGVL CLKTNKPRSS LGVSKDEHSW SVSLRIGPI
	Isoform 3. Checksum: 56976D2DF52B8BD2	FASTA	527	59,568
	10 20 30 40 50 60 MIDFLQNHTE GTWPPACPPL DPIQSSDILS FMDSHSGQYH AIVFESNGSY VGREVILDLI 70 80 90 100 110 120 PYENIMVSRA LDTDKAFLGT LGITSVPSCY LIYPNGSHGL VNVAKPLRSF FSSHLKSLPD 130 140 150 160 170 180 VRKKSLFLPE KSNKEEKSEV VVWKEFDRAK LYTADLESGL HYLLRVELAA HRSLAGAQLK 190 200 210 220 230 240 TFRDFVTVVA KLFPGRPAVK KLLETLQEWL ANLPLDKIPY NAILDLVNNK MQISGIFLTS 250 260 270 280 290 300 HVKWVGCQGS RLELRGYPCS LWKLFHTLTV QASTHPEALA GTGFEGHPQA VLQAIRRYIR 310 320 330 340 350 360 TFFGCKECGE HFEEMAKESM DSVKTPDQAV LWLWRKHNMV NSRLAGHLSE DPKFPKVPWP 370 380 390 400 410 420 TPDLCPACHE EIKGLDSWNE GQVLLFLKQH YSRDNLVDAY SVDQGSPGEW EAQGREQEEG 430 440 450 460 470 480 KGLNPSGKSW RHHDTGSLRP PHILGPRTDL SKSLHHRLDL RLQSPQGPQA LKEAKAVVPF 490 500 510 520 LGVGFSSLDM SLCVVLYVAS SLFLMIMYFF FRVRSKRWKV RLYHPAV

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Adipose tissue, Inner ear and Thymus.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK042036 mRNA. Translation: BAC31140.1. Sequence problems. AK140324 mRNA. Translation: BAE24335.1. AK157992 mRNA. Translation: BAE34304.1. AK165642 mRNA. Translation: BAE38312.1. BC030934 mRNA. Translation: AAH30934.1.
IPI	IPI00169589. IPI00474916. IPI00788406.
UniGene	Mm.116769
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q3TMX7.
Genome annotation databases
Ensembl	ENSMUSG00000036327. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:2387194. Qsox2.
Phylogenomic databases
HOVERGEN	Q3TMX7.
OMA	Q3TMX7. EEMATES.
Enzyme and pathway databases
BRENDA	1.8.3.2. 244.
Gene expression databases
Bgee	Q3TMX7.
CleanEx	MM_QSOX2.
GermOnline	ENSMUSG00000036327. Mus musculus.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. IPR017936. Thioredoxin-like. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS51324. ERV_ALR. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

QSOX2_MOUSE

Accession

Primary (citable) accession number: Q3TMX7
Secondary accession number(s): Q3TZA5, Q8C9I4, Q8K0M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	October 11, 2005
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)