Q3TLD5 (RMP_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 57. History...
Names and origin
|Protein names||Recommended name:|
Unconventional prefoldin RPB5 interactor
Protein phosphatase 1 regulatory subunit 19
RNA polymerase II subunit 5-mediating protein
Short name=RPB5-mediating protein
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||531 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region By similarity.
Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination By similarity.
Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT By similarity.
Expressed in the spinal cord, ganglia, choroid plexus and olfactors epithelium of the developing brain. Expressed in skin, lung, kidney, testis and muscles (at protein level). Expressed strongly in brain and kidney. Expressed weakly in skeletal muscle, lung and liver. Ref.1
Expressed in embryo at 6 dpc, onward. Ref.1
Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-369 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-369 disrupts the URI1-PPP1CC complex in the mitochondrion, relieves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity, preventing sustained S6K1-dependent signaling By similarity. Phosphorylated. Phosphorylation occurs essentially on serine residues. Ref.1
Belongs to the RNA polymerase II subunit 5-mediating protein family.
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: Q3TLD5-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: Q3TLD5-2) |
The sequence of this isoform differs from the canonical sequence as follows:
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 531||531||Unconventional prefoldin RPB5 interactor||PRO_0000328753|
Amino acid modifications
|Modified residue||242||1||Phosphoserine Ref.4|
|Modified residue||369||1||Phosphoserine; by RPS6KB1 By similarity|
|Modified residue||437||1||Phosphoserine Ref.4|
|Alternative sequence||1 – 74||74||Missing in isoform 2.||VSP_032774|
|Sequence conflict||143||1||T → A in BAE38857. Ref.1|
|Sequence conflict||201||1||N → D in AAH23029. Ref.3|
|Sequence conflict||306||1||E → D in AAH23029. Ref.3|
|Sequence conflict||387||1||D → E in AAD08680. Ref.1|
|Sequence conflict||403||1||R → H in AAH23029. Ref.3|
|||"Molecular cloning of a gene on chromosome 19q12 coding for a novel intracellular protein: analysis of expression in human and mouse tissues and in human tumor cells, particularly Reed-Sternberg cells in Hodgkin disease."|
Van Leuven F., Torrekens S., Moechars D., Hilliker C., Buellens M., Bollen M., Delabie J.
Genomics 54:511-520(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PHOSPHORYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
|||"The transcriptional landscape of the mammalian genome."|
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary tumor.
|||"Large-scale phosphorylation analysis of mouse liver."|
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242 AND SER-437, MASS SPECTROMETRY.
|+||Additional computationally mapped references.|
|AF091096 mRNA. Translation: AAD08680.1.|
AK133383 mRNA. Translation: BAE21626.1.
AK166566 mRNA. Translation: BAE38857.1.
BC023029 mRNA. Translation: AAH23029.1.
|RefSeq||NP_035404.4. NM_011274.5. |
3D structure databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSMUST00000085513; ENSMUSP00000082646; ENSMUSG00000030421. |
|UCSC||uc009gkp.2. mouse. |
|MGI||MGI:1342294. Uri1. |
Gene expression databases
Family and domain databases
|InterPro||IPR009053. Prefoldin. |
|Pfam||PF02996. Prefoldin. 1 hit. |
|SUPFAM||SSF46579. Prefoldin. 1 hit. |
|Accession||Primary (citable) accession number: Q3TLD5|
Secondary accession number(s): Q3V078, Q8R5C4, Q9Z243
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|