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Q3TLD5

- RMP_MOUSE

UniProt

Q3TLD5 - RMP_MOUSE

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Protein

Unconventional prefoldin RPB5 interactor

Gene

Uri1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region (By similarity).By similarity
Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination (By similarity).By similarity

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. protein phosphatase inhibitor activity Source: UniProtKB-KW
  3. RNA polymerase II transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. cellular response to growth factor stimulus Source: UniProtKB
  2. cellular response to steroid hormone stimulus Source: UniProtKB
  3. negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  4. negative regulation of phosphatase activity Source: UniProtKB
  5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  6. protein folding Source: InterPro
  7. regulation of cell growth Source: UniProtKB
  8. response to virus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional prefoldin RPB5 interactor
Alternative name(s):
Protein NNX3
Protein phosphatase 1 regulatory subunit 19
RNA polymerase II subunit 5-mediating protein
Short name:
RPB5-mediating protein
Gene namesi
Name:Uri1
Synonyms:Nnx3, Ppp1r19, Rmp, Uri
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1342294. Uri1.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Mitochondrion 1 Publication. Cell projectiondendrite By similarity
Note: Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 in mitochondrion.

GO - Cellular componenti

  1. cell projection Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB
  3. DNA-directed RNA polymerase II, core complex Source: Ensembl
  4. mitochondrion Source: UniProtKB-KW
  5. nucleus Source: UniProtKB
  6. prefoldin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Unconventional prefoldin RPB5 interactorPRO_0000328753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei369 – 3691Phosphoserine; by RPS6KB1By similarity

Post-translational modificationi

Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-369 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-369 disrupts the URI1-PPP1CC complex in the mitochondrion, relieves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity, preventing sustained S6K1-dependent signaling (By similarity). Phosphorylated. Phosphorylation occurs essentially on serine residues.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ3TLD5.
PaxDbiQ3TLD5.
PRIDEiQ3TLD5.

PTM databases

PhosphoSiteiQ3TLD5.

Expressioni

Tissue specificityi

Expressed in the spinal cord, ganglia, choroid plexus and olfactors epithelium of the developing brain. Expressed in skin, lung, kidney, testis and muscles (at protein level). Expressed strongly in brain and kidney. Expressed weakly in skeletal muscle, lung and liver.1 Publication

Developmental stagei

Expressed in embryo at 6 dpc, onward.1 Publication

Gene expression databases

BgeeiQ3TLD5.
ExpressionAtlasiQ3TLD5. baseline and differential.
GenevestigatoriQ3TLD5.

Interactioni

Subunit structurei

Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3TLD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG331327.
GeneTreeiENSGT00390000002362.
HOGENOMiHOG000154150.
HOVERGENiHBG007610.
InParanoidiQ3TLD5.
KOiK17560.
OMAiIPRKSIL.
OrthoDBiEOG7PZRZV.
PhylomeDBiQ3TLD5.
TreeFamiTF332816.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PfamiPF02996. Prefoldin. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3TLD5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPPSEPEPE PQPLAEASAA APLRAPEVAR LREEQEKVVT NCQEKIQHWE
60 70 80 90 100
KVDNDYSALQ ERLRTLPDKL SYDVMVPFGP LAFMPGKLVH TNEVTVLLGD
110 120 130 140 150
NWFAKCSAKQ AVGLVEHRKE HVRKTIDDFK KVLKNFESRV EFTEDLQKMS
160 170 180 190 200
DAAGDFVDIR EEIKSDFEFK GKQRIAHKPH SKPKTSDIFE ADFENGVKPK
210 220 230 240 250
NTFDADELWA RLEELERQEE LLGELESKPD TVIANGEDRV SSEEEKEGAD
260 270 280 290 300
TGVNVVSPVT DSSAASSCKR RAGNAGLPNG QVNSLNYSVN GSNSYHSNKD
310 320 330 340 350
DDEEEEDDDD DDDEDDDNES DHAISADNSI PTIYFSHTVE PKRVRINTGK
360 370 380 390 400
NTTLKFSEKK EEAKRKRKSG AGSHATHELP AIKSPADIYR VFVDVVNGEY
410 420 430 440 450
VPRKSILKSR SRENSVCSDT SESSAADVED RRGLLRSTSS EEAVATEAGG
460 470 480 490 500
SSLDELQENH PKKPLPSGVS EAFSGTVIEK EFLSPSLAPY SAIAHHALPT
510 520 530
IPERKEVPSE VSEEPTKRVS KFRAARLQQR S
Length:531
Mass (Da):59,084
Last modified:April 8, 2008 - v2
Checksum:i47624F5450813693
GO
Isoform 2 (identifier: Q3TLD5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Show »
Length:457
Mass (Da):50,634
Checksum:i5BCCA41FC89E1261
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431T → A in BAE38857. (PubMed:9878255)Curated
Sequence conflicti201 – 2011N → D in AAH23029. (PubMed:15489334)Curated
Sequence conflicti306 – 3061E → D in AAH23029. (PubMed:15489334)Curated
Sequence conflicti387 – 3871D → E in AAD08680. (PubMed:9878255)Curated
Sequence conflicti403 – 4031R → H in AAH23029. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7474Missing in isoform 2. 3 PublicationsVSP_032774Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091096 mRNA. Translation: AAD08680.1.
AK133383 mRNA. Translation: BAE21626.1.
AK166566 mRNA. Translation: BAE38857.1.
BC023029 mRNA. Translation: AAH23029.1.
CCDSiCCDS21157.1. [Q3TLD5-1]
RefSeqiNP_035404.4. NM_011274.5. [Q3TLD5-1]
XP_006539741.1. XM_006539678.1. [Q3TLD5-2]
UniGeneiMm.387220.

Genome annotation databases

EnsembliENSMUST00000085513; ENSMUSP00000082646; ENSMUSG00000030421. [Q3TLD5-1]
GeneIDi19777.
KEGGimmu:19777.
UCSCiuc009gkp.2. mouse. [Q3TLD5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091096 mRNA. Translation: AAD08680.1 .
AK133383 mRNA. Translation: BAE21626.1 .
AK166566 mRNA. Translation: BAE38857.1 .
BC023029 mRNA. Translation: AAH23029.1 .
CCDSi CCDS21157.1. [Q3TLD5-1 ]
RefSeqi NP_035404.4. NM_011274.5. [Q3TLD5-1 ]
XP_006539741.1. XM_006539678.1. [Q3TLD5-2 ]
UniGenei Mm.387220.

3D structure databases

ProteinModelPortali Q3TLD5.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q3TLD5.

Proteomic databases

MaxQBi Q3TLD5.
PaxDbi Q3TLD5.
PRIDEi Q3TLD5.

Protocols and materials databases

DNASUi 19777.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000085513 ; ENSMUSP00000082646 ; ENSMUSG00000030421 . [Q3TLD5-1 ]
GeneIDi 19777.
KEGGi mmu:19777.
UCSCi uc009gkp.2. mouse. [Q3TLD5-1 ]

Organism-specific databases

CTDi 8725.
MGIi MGI:1342294. Uri1.

Phylogenomic databases

eggNOGi NOG331327.
GeneTreei ENSGT00390000002362.
HOGENOMi HOG000154150.
HOVERGENi HBG007610.
InParanoidi Q3TLD5.
KOi K17560.
OMAi IPRKSIL.
OrthoDBi EOG7PZRZV.
PhylomeDBi Q3TLD5.
TreeFami TF332816.

Miscellaneous databases

NextBioi 297242.
PROi Q3TLD5.
SOURCEi Search...

Gene expression databases

Bgeei Q3TLD5.
ExpressionAtlasi Q3TLD5. baseline and differential.
Genevestigatori Q3TLD5.

Family and domain databases

Gene3Di 1.10.287.370. 1 hit.
InterProi IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view ]
Pfami PF02996. Prefoldin. 1 hit.
[Graphical view ]
SUPFAMi SSF46579. SSF46579. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a gene on chromosome 19q12 coding for a novel intracellular protein: analysis of expression in human and mouse tissues and in human tumor cells, particularly Reed-Sternberg cells in Hodgkin disease."
    Van Leuven F., Torrekens S., Moechars D., Hilliker C., Buellens M., Bollen M., Delabie J.
    Genomics 54:511-520(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PHOSPHORYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRMP_MOUSE
AccessioniPrimary (citable) accession number: Q3TLD5
Secondary accession number(s): Q3V078, Q8R5C4, Q9Z243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3