Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3TLD5

- RMP_MOUSE

UniProt

Q3TLD5 - RMP_MOUSE

Protein

Unconventional prefoldin RPB5 interactor

Gene

Uri1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (08 Apr 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region By similarity.By similarity
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination By similarity.By similarity

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. protein phosphatase inhibitor activity Source: UniProtKB-KW
    3. RNA polymerase II transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to growth factor stimulus Source: UniProtKB
    2. cellular response to steroid hormone stimulus Source: UniProtKB
    3. negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    4. negative regulation of phosphatase activity Source: UniProtKB
    5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    6. protein folding Source: InterPro
    7. regulation of cell growth Source: UniProtKB
    8. response to virus Source: Ensembl

    Keywords - Molecular functioni

    Protein phosphatase inhibitor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Unconventional prefoldin RPB5 interactor
    Alternative name(s):
    Protein NNX3
    Protein phosphatase 1 regulatory subunit 19
    RNA polymerase II subunit 5-mediating protein
    Short name:
    RPB5-mediating protein
    Gene namesi
    Name:Uri1
    Synonyms:Nnx3, Ppp1r19, Rmp, Uri
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1342294. Uri1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Mitochondrion 1 Publication. Cell projectiondendrite By similarity
    Note: Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 in mitochondrion.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. dendrite Source: UniProtKB-SubCell
    3. DNA-directed RNA polymerase II, core complex Source: Ensembl
    4. mitochondrion Source: UniProtKB-SubCell
    5. nucleus Source: UniProtKB
    6. prefoldin complex Source: InterPro

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 531531Unconventional prefoldin RPB5 interactorPRO_0000328753Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei369 – 3691Phosphoserine; by RPS6KB1By similarity

    Post-translational modificationi

    Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-369 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-369 disrupts the URI1-PPP1CC complex in the mitochondrion, relieves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity, preventing sustained S6K1-dependent signaling By similarity. Phosphorylated. Phosphorylation occurs essentially on serine residues.By similarity1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ3TLD5.
    PaxDbiQ3TLD5.
    PRIDEiQ3TLD5.

    PTM databases

    PhosphoSiteiQ3TLD5.

    Expressioni

    Tissue specificityi

    Expressed in the spinal cord, ganglia, choroid plexus and olfactors epithelium of the developing brain. Expressed in skin, lung, kidney, testis and muscles (at protein level). Expressed strongly in brain and kidney. Expressed weakly in skeletal muscle, lung and liver.1 Publication

    Developmental stagei

    Expressed in embryo at 6 dpc, onward.1 Publication

    Gene expression databases

    ArrayExpressiQ3TLD5.
    BgeeiQ3TLD5.
    GenevestigatoriQ3TLD5.

    Interactioni

    Subunit structurei

    Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TLD5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG331327.
    GeneTreeiENSGT00390000002362.
    HOGENOMiHOG000154150.
    HOVERGENiHBG007610.
    InParanoidiQ3TLD5.
    KOiK17560.
    OMAiIPRKSIL.
    OrthoDBiEOG7PZRZV.
    PhylomeDBiQ3TLD5.
    TreeFamiTF332816.

    Family and domain databases

    Gene3Di1.10.287.370. 1 hit.
    InterProiIPR009053. Prefoldin.
    IPR004127. Prefoldin_subunit_alpha.
    [Graphical view]
    PfamiPF02996. Prefoldin. 1 hit.
    [Graphical view]
    SUPFAMiSSF46579. SSF46579. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3TLD5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPPSEPEPE PQPLAEASAA APLRAPEVAR LREEQEKVVT NCQEKIQHWE    50
    KVDNDYSALQ ERLRTLPDKL SYDVMVPFGP LAFMPGKLVH TNEVTVLLGD 100
    NWFAKCSAKQ AVGLVEHRKE HVRKTIDDFK KVLKNFESRV EFTEDLQKMS 150
    DAAGDFVDIR EEIKSDFEFK GKQRIAHKPH SKPKTSDIFE ADFENGVKPK 200
    NTFDADELWA RLEELERQEE LLGELESKPD TVIANGEDRV SSEEEKEGAD 250
    TGVNVVSPVT DSSAASSCKR RAGNAGLPNG QVNSLNYSVN GSNSYHSNKD 300
    DDEEEEDDDD DDDEDDDNES DHAISADNSI PTIYFSHTVE PKRVRINTGK 350
    NTTLKFSEKK EEAKRKRKSG AGSHATHELP AIKSPADIYR VFVDVVNGEY 400
    VPRKSILKSR SRENSVCSDT SESSAADVED RRGLLRSTSS EEAVATEAGG 450
    SSLDELQENH PKKPLPSGVS EAFSGTVIEK EFLSPSLAPY SAIAHHALPT 500
    IPERKEVPSE VSEEPTKRVS KFRAARLQQR S 531
    Length:531
    Mass (Da):59,084
    Last modified:April 8, 2008 - v2
    Checksum:i47624F5450813693
    GO
    Isoform 2 (identifier: Q3TLD5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-74: Missing.

    Show »
    Length:457
    Mass (Da):50,634
    Checksum:i5BCCA41FC89E1261
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti143 – 1431T → A in BAE38857. (PubMed:9878255)Curated
    Sequence conflicti201 – 2011N → D in AAH23029. (PubMed:15489334)Curated
    Sequence conflicti306 – 3061E → D in AAH23029. (PubMed:15489334)Curated
    Sequence conflicti387 – 3871D → E in AAD08680. (PubMed:9878255)Curated
    Sequence conflicti403 – 4031R → H in AAH23029. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7474Missing in isoform 2. 3 PublicationsVSP_032774Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF091096 mRNA. Translation: AAD08680.1.
    AK133383 mRNA. Translation: BAE21626.1.
    AK166566 mRNA. Translation: BAE38857.1.
    BC023029 mRNA. Translation: AAH23029.1.
    CCDSiCCDS21157.1. [Q3TLD5-1]
    RefSeqiNP_035404.4. NM_011274.5. [Q3TLD5-1]
    XP_006539741.1. XM_006539678.1. [Q3TLD5-2]
    UniGeneiMm.387220.

    Genome annotation databases

    EnsembliENSMUST00000085513; ENSMUSP00000082646; ENSMUSG00000030421. [Q3TLD5-1]
    GeneIDi19777.
    KEGGimmu:19777.
    UCSCiuc009gkp.2. mouse. [Q3TLD5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF091096 mRNA. Translation: AAD08680.1 .
    AK133383 mRNA. Translation: BAE21626.1 .
    AK166566 mRNA. Translation: BAE38857.1 .
    BC023029 mRNA. Translation: AAH23029.1 .
    CCDSi CCDS21157.1. [Q3TLD5-1 ]
    RefSeqi NP_035404.4. NM_011274.5. [Q3TLD5-1 ]
    XP_006539741.1. XM_006539678.1. [Q3TLD5-2 ]
    UniGenei Mm.387220.

    3D structure databases

    ProteinModelPortali Q3TLD5.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q3TLD5.

    Proteomic databases

    MaxQBi Q3TLD5.
    PaxDbi Q3TLD5.
    PRIDEi Q3TLD5.

    Protocols and materials databases

    DNASUi 19777.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085513 ; ENSMUSP00000082646 ; ENSMUSG00000030421 . [Q3TLD5-1 ]
    GeneIDi 19777.
    KEGGi mmu:19777.
    UCSCi uc009gkp.2. mouse. [Q3TLD5-1 ]

    Organism-specific databases

    CTDi 8725.
    MGIi MGI:1342294. Uri1.

    Phylogenomic databases

    eggNOGi NOG331327.
    GeneTreei ENSGT00390000002362.
    HOGENOMi HOG000154150.
    HOVERGENi HBG007610.
    InParanoidi Q3TLD5.
    KOi K17560.
    OMAi IPRKSIL.
    OrthoDBi EOG7PZRZV.
    PhylomeDBi Q3TLD5.
    TreeFami TF332816.

    Miscellaneous databases

    NextBioi 297242.
    PROi Q3TLD5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q3TLD5.
    Bgeei Q3TLD5.
    Genevestigatori Q3TLD5.

    Family and domain databases

    Gene3Di 1.10.287.370. 1 hit.
    InterProi IPR009053. Prefoldin.
    IPR004127. Prefoldin_subunit_alpha.
    [Graphical view ]
    Pfami PF02996. Prefoldin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46579. SSF46579. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a gene on chromosome 19q12 coding for a novel intracellular protein: analysis of expression in human and mouse tissues and in human tumor cells, particularly Reed-Sternberg cells in Hodgkin disease."
      Van Leuven F., Torrekens S., Moechars D., Hilliker C., Buellens M., Bollen M., Delabie J.
      Genomics 54:511-520(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PHOSPHORYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRMP_MOUSE
    AccessioniPrimary (citable) accession number: Q3TLD5
    Secondary accession number(s): Q3V078, Q8R5C4, Q9Z243
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3