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Protein

Dimethyladenosine transferase 2, mitochondrial

Gene

Tfb2m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei123S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei149S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-163282. Mitochondrial transcription initiation.
R-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 2, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 2
Mitochondrial transcription factor B2
Short name:
mTFB2M
Short name:
mtTFB2
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
Gene namesi
Name:Tfb2m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107937. Tfb2m.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrial nucleoid Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionSequence analysisAdd BLAST43
ChainiPRO_000027318044 – 396Dimethyladenosine transferase 2, mitochondrialAdd BLAST353

Proteomic databases

EPDiQ3TL26.
MaxQBiQ3TL26.
PaxDbiQ3TL26.
PeptideAtlasiQ3TL26.
PRIDEiQ3TL26.

PTM databases

iPTMnetiQ3TL26.
PhosphoSitePlusiQ3TL26.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000026492.
CleanExiMM_TFB2M.
ExpressionAtlasiQ3TL26. baseline and differential.
GenevisibleiQ3TL26. MM.

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027769.

Structurei

3D structure databases

ProteinModelPortaliQ3TL26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0820. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00510000048533.
HOGENOMiHOG000060174.
HOVERGENiHBG094037.
InParanoidiQ3TL26.
KOiK17653.
OMAiTLLMMIK.
OrthoDBiEOG091G0DOP.
PhylomeDBiQ3TL26.
TreeFamiTF325100.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
IPR016861. TFB2M.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 2 hits.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
PIRSFiPIRSF027833. MtTFB2. 1 hit.
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3TL26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGPAMRLPP RIALSALARG PSCILGSGAA TRKDWQTRNR RGFSDFNIEP
60 70 80 90 100
LPDSDLEESS PWTSRNRSEP TRHIACKKAA RNLVRDLLEH QNPSRQIILE
110 120 130 140 150
CNPGPGILTG ALLKAGARVV AFESEKTFIP HLEPLQRNMD GELQVVHCDF
160 170 180 190 200
FKMDPRYQEV VRPDVSSQAI FQNLGIKAVP WSAGVPIKVF GILPYKHERR
210 220 230 240 250
ILWKILFDLY SCESIYRYGR VELNMFVSEK EFRKLIATPK RPDLYQVMAV
260 270 280 290 300
LWQVACDVKF LHMEPWSSFS VHTENGHLEK SKHGESVNLL KQNLYLVRMT
310 320 330 340 350
PRRTLFTENL SPLNYDIFFH LVKHCFGKRN APIIRHLRSL STVDPINILR
360 370 380 390
QIRKNPGDTA ARMYPHDFKK LFETIEQSED SVFKWIYDYC PEDMEF
Length:396
Mass (Da):45,864
Last modified:October 11, 2005 - v1
Checksum:iD276BD58D595F111
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12I → L in AAA37817 (Ref. 1) Curated1
Sequence conflicti40R → G in AAA37817 (Ref. 1) Curated1
Sequence conflicti273T → M in AAA37817 (Ref. 1) Curated1
Sequence conflicti292Q → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti308E → G in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti324H → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti330N → H in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti338R → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti377Q → P in BAC41095 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74555 mRNA. Translation: AAA37817.1.
AK090106 mRNA. Translation: BAC41095.1.
AK146563 mRNA. Translation: BAE27262.1.
AK166717 mRNA. Translation: BAE38967.1.
AK167930 mRNA. Translation: BAE39934.1.
AK168970 mRNA. Translation: BAE40774.1.
AK169138 mRNA. Translation: BAE40917.1.
CCDSiCCDS15561.1.
PIRiS27870.
RefSeqiNP_001317984.1. NM_001331055.1.
NP_032275.2. NM_008249.5.
UniGeneiMm.293529.
Mm.390474.

Genome annotation databases

EnsembliENSMUST00000027769; ENSMUSP00000027769; ENSMUSG00000026492.
GeneIDi15278.
KEGGimmu:15278.
UCSCiuc007dvl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74555 mRNA. Translation: AAA37817.1.
AK090106 mRNA. Translation: BAC41095.1.
AK146563 mRNA. Translation: BAE27262.1.
AK166717 mRNA. Translation: BAE38967.1.
AK167930 mRNA. Translation: BAE39934.1.
AK168970 mRNA. Translation: BAE40774.1.
AK169138 mRNA. Translation: BAE40917.1.
CCDSiCCDS15561.1.
PIRiS27870.
RefSeqiNP_001317984.1. NM_001331055.1.
NP_032275.2. NM_008249.5.
UniGeneiMm.293529.
Mm.390474.

3D structure databases

ProteinModelPortaliQ3TL26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027769.

PTM databases

iPTMnetiQ3TL26.
PhosphoSitePlusiQ3TL26.

Proteomic databases

EPDiQ3TL26.
MaxQBiQ3TL26.
PaxDbiQ3TL26.
PeptideAtlasiQ3TL26.
PRIDEiQ3TL26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027769; ENSMUSP00000027769; ENSMUSG00000026492.
GeneIDi15278.
KEGGimmu:15278.
UCSCiuc007dvl.1. mouse.

Organism-specific databases

CTDi64216.
MGIiMGI:107937. Tfb2m.

Phylogenomic databases

eggNOGiKOG0820. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00510000048533.
HOGENOMiHOG000060174.
HOVERGENiHBG094037.
InParanoidiQ3TL26.
KOiK17653.
OMAiTLLMMIK.
OrthoDBiEOG091G0DOP.
PhylomeDBiQ3TL26.
TreeFamiTF325100.

Enzyme and pathway databases

ReactomeiR-MMU-163282. Mitochondrial transcription initiation.
R-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

PROiQ3TL26.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026492.
CleanExiMM_TFB2M.
ExpressionAtlasiQ3TL26. baseline and differential.
GenevisibleiQ3TL26. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
IPR016861. TFB2M.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 2 hits.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
PIRSFiPIRSF027833. MtTFB2. 1 hit.
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFB2M_MOUSE
AccessioniPrimary (citable) accession number: Q3TL26
Secondary accession number(s): Q61669, Q8BTJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.