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Protein

Dimethyladenosine transferase 2, mitochondrial

Gene

Tfb2m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei123S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei149S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-163282 Mitochondrial transcription initiation

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 2, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 2
Mitochondrial transcription factor B2
Short name:
mTFB2M
Short name:
mtTFB2
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
Gene namesi
Name:Tfb2m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107937 Tfb2m

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionSequence analysisAdd BLAST43
ChainiPRO_000027318044 – 396Dimethyladenosine transferase 2, mitochondrialAdd BLAST353

Proteomic databases

EPDiQ3TL26
MaxQBiQ3TL26
PaxDbiQ3TL26
PeptideAtlasiQ3TL26
PRIDEiQ3TL26

PTM databases

iPTMnetiQ3TL26
PhosphoSitePlusiQ3TL26

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000026492
CleanExiMM_TFB2M
ExpressionAtlasiQ3TL26 baseline and differential
GenevisibleiQ3TL26 MM

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

BioGridi200317, 1 interactor
STRINGi10090.ENSMUSP00000027769

Structurei

3D structure databases

ProteinModelPortaliQ3TL26
SMRiQ3TL26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0820 Eukaryota
COG0030 LUCA
GeneTreeiENSGT00510000048533
HOGENOMiHOG000060174
HOVERGENiHBG094037
InParanoidiQ3TL26
KOiK17653
OMAiFIPHLEX
OrthoDBiEOG091G0DOP
PhylomeDBiQ3TL26
TreeFamiTF325100

Family and domain databases

InterProiView protein in InterPro
IPR001737 KsgA/Erm
IPR020598 rRNA_Ade_methylase_Trfase_N
IPR029063 SAM-dependent_MTases
IPR016861 TFB2M
PANTHERiPTHR11727 PTHR11727, 1 hit
PTHR11727:SF13 PTHR11727:SF13, 1 hit
PfamiView protein in Pfam
PF00398 RrnaAD, 1 hit
SMARTiView protein in SMART
SM00650 rADc, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51689 SAM_RNA_A_N6_MT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3TL26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGPAMRLPP RIALSALARG PSCILGSGAA TRKDWQTRNR RGFSDFNIEP
60 70 80 90 100
LPDSDLEESS PWTSRNRSEP TRHIACKKAA RNLVRDLLEH QNPSRQIILE
110 120 130 140 150
CNPGPGILTG ALLKAGARVV AFESEKTFIP HLEPLQRNMD GELQVVHCDF
160 170 180 190 200
FKMDPRYQEV VRPDVSSQAI FQNLGIKAVP WSAGVPIKVF GILPYKHERR
210 220 230 240 250
ILWKILFDLY SCESIYRYGR VELNMFVSEK EFRKLIATPK RPDLYQVMAV
260 270 280 290 300
LWQVACDVKF LHMEPWSSFS VHTENGHLEK SKHGESVNLL KQNLYLVRMT
310 320 330 340 350
PRRTLFTENL SPLNYDIFFH LVKHCFGKRN APIIRHLRSL STVDPINILR
360 370 380 390
QIRKNPGDTA ARMYPHDFKK LFETIEQSED SVFKWIYDYC PEDMEF
Length:396
Mass (Da):45,864
Last modified:October 11, 2005 - v1
Checksum:iD276BD58D595F111
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12I → L in AAA37817 (Ref. 1) Curated1
Sequence conflicti40R → G in AAA37817 (Ref. 1) Curated1
Sequence conflicti273T → M in AAA37817 (Ref. 1) Curated1
Sequence conflicti292Q → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti308E → G in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti324H → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti330N → H in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti338R → P in BAC41095 (PubMed:16141072).Curated1
Sequence conflicti377Q → P in BAC41095 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74555 mRNA Translation: AAA37817.1
AK090106 mRNA Translation: BAC41095.1
AK146563 mRNA Translation: BAE27262.1
AK166717 mRNA Translation: BAE38967.1
AK167930 mRNA Translation: BAE39934.1
AK168970 mRNA Translation: BAE40774.1
AK169138 mRNA Translation: BAE40917.1
CCDSiCCDS15561.1
PIRiS27870
RefSeqiNP_001317984.1, NM_001331055.1
NP_032275.2, NM_008249.5
UniGeneiMm.293529
Mm.390474

Genome annotation databases

EnsembliENSMUST00000027769; ENSMUSP00000027769; ENSMUSG00000026492
GeneIDi15278
KEGGimmu:15278
UCSCiuc007dvl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTFB2M_MOUSE
AccessioniPrimary (citable) accession number: Q3TL26
Secondary accession number(s): Q61669, Q8BTJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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