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Q3TJM4

- CENPT_MOUSE

UniProt

Q3TJM4 - CENPT_MOUSE

Protein

Centromere protein T

Gene

Cenpt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromosome organization Source: UniProtKB
    2. chromosome segregation Source: UniProtKB
    3. kinetochore assembly Source: UniProtKB
    4. mitotic cell cycle Source: UniProtKB
    5. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein T
    Short name:
    CENP-T
    Gene namesi
    Name:Cenpt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:2443939. Cenpt.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
    Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus By similarity.By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB
    2. condensed chromosome kinetochore Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 515515Centromere protein TPRO_0000249516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 861PhosphothreonineBy similarity
    Modified residuei333 – 3331PhosphoserineBy similarity
    Modified residuei345 – 3451PhosphoserineBy similarity
    Modified residuei346 – 3461PhosphoserineBy similarity
    Modified residuei357 – 3571PhosphoserineBy similarity

    Post-translational modificationi

    Dynamically phosphorylated during the cell cycle. Phosphorylated during G2 phase, metaphase and anaphase, but not during telophase or G1 phase.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ3TJM4.

    Expressioni

    Gene expression databases

    BgeeiQ3TJM4.
    CleanExiMM_CENPT.
    GenevestigatoriQ3TJM4.

    Interactioni

    Subunit structurei

    Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Part of a centromere complex consisting of CENPA, CENPT and CENPW. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Interacts (via N-terminus) with the NDC80 complex By similarity. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Binds DNA By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TJM4.
    SMRiQ3TJM4. Positions 419-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 375282Flexible stalk domainBy similarityAdd
    BLAST

    Domaini

    The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.By similarity

    Phylogenomic databases

    eggNOGiNOG26624.
    GeneTreeiENSGT00390000003044.
    HOGENOMiHOG000111545.
    HOVERGENiHBG081089.
    InParanoidiQ3TJM4.
    KOiK11512.
    OMAiFSFYAKM.
    OrthoDBiEOG7TQV2Z.
    PhylomeDBiQ3TJM4.
    TreeFamiTF332946.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR028255. CENP-T.
    IPR009072. Histone-fold.
    [Graphical view]
    PANTHERiPTHR14857. PTHR14857. 1 hit.
    SUPFAMiSSF47113. SSF47113. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3TJM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLSFSDGD PTVRTLLRRV LETADSRTPM RRRSTRINAQ RRRSQTPYSN    50
    RQGSQTKTSA RKQSHGARSV GRSTRVQGRG RLEEQTPRTL LRNILLTAPE 100
    SSTVMPDPVV KPAQVPEVAR SSRRESSRGS LELHLPELEP PSTLAPGLTA 150
    PGKRKQKLRL SVFQQEVDQG LPLSQEPRRS RSADVSSLAS SFNLTFVLPG 200
    QPETVERPGL ARRRPIRQLV NAGALLQDLE DNSLASALPG DSHRTPVAAL 250
    PMDVGLEDTQ PFSQSLAAFS LSGKHSLPSP SRPGVEDVER VMGPPSSGTR 300
    LQSRMSRSGP AASPSPFLEP QPPPAEPREA VGSNEAAEPK DQEGSSGYEE 350
    TSARPASGEL SSSTHDSLPA EQPPPSPGVA VLSSEPLESV TAKCPSRTQT 400
    AGPRRRQDPH KAGLSPYVKF FSFCTKMPVE KTALEIVEKC LDKYFQHLCN 450
    DLEVFASHAG RKIVKPEDLL LLMRRQGLVT DQVSQHVLVE RYLPLEYRQQ 500
    LIPCAFSGNS VFPAQ 515
    Length:515
    Mass (Da):56,242
    Last modified:October 3, 2006 - v2
    Checksum:iC736E7FA30E6206B
    GO

    Sequence cautioni

    The sequence BAC40776.1 differs from that shown. Reason: Frameshift at position 303.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51S → V in BAE25464. (PubMed:16141072)Curated
    Sequence conflicti157 – 1571K → R in BAE39471. (PubMed:16141072)Curated
    Sequence conflicti302 – 3032QS → KG in BAC40776. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK089172 mRNA. Translation: BAC40776.1. Frameshift.
    AK090340 mRNA. Translation: BAC41176.1.
    AK143613 mRNA. Translation: BAE25464.1.
    AK164039 mRNA. Translation: BAE37599.1.
    AK167376 mRNA. Translation: BAE39471.1.
    BC022690 mRNA. Translation: AAH22690.1.
    BC121824 mRNA. Translation: AAI21825.1.
    CCDSiCCDS22615.1.
    RefSeqiNP_796124.1. NM_177150.2.
    UniGeneiMm.334775.

    Genome annotation databases

    EnsembliENSMUST00000040776; ENSMUSP00000038188; ENSMUSG00000036672.
    GeneIDi320394.
    KEGGimmu:320394.
    UCSCiuc009nef.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK089172 mRNA. Translation: BAC40776.1 . Frameshift.
    AK090340 mRNA. Translation: BAC41176.1 .
    AK143613 mRNA. Translation: BAE25464.1 .
    AK164039 mRNA. Translation: BAE37599.1 .
    AK167376 mRNA. Translation: BAE39471.1 .
    BC022690 mRNA. Translation: AAH22690.1 .
    BC121824 mRNA. Translation: AAI21825.1 .
    CCDSi CCDS22615.1.
    RefSeqi NP_796124.1. NM_177150.2.
    UniGenei Mm.334775.

    3D structure databases

    ProteinModelPortali Q3TJM4.
    SMRi Q3TJM4. Positions 419-505.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q3TJM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040776 ; ENSMUSP00000038188 ; ENSMUSG00000036672 .
    GeneIDi 320394.
    KEGGi mmu:320394.
    UCSCi uc009nef.1. mouse.

    Organism-specific databases

    CTDi 80152.
    MGIi MGI:2443939. Cenpt.

    Phylogenomic databases

    eggNOGi NOG26624.
    GeneTreei ENSGT00390000003044.
    HOGENOMi HOG000111545.
    HOVERGENi HBG081089.
    InParanoidi Q3TJM4.
    KOi K11512.
    OMAi FSFYAKM.
    OrthoDBi EOG7TQV2Z.
    PhylomeDBi Q3TJM4.
    TreeFami TF332946.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 396642.
    PROi Q3TJM4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3TJM4.
    CleanExi MM_CENPT.
    Genevestigatori Q3TJM4.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR028255. CENP-T.
    IPR009072. Histone-fold.
    [Graphical view ]
    PANTHERi PTHR14857. PTHR14857. 1 hit.
    SUPFAMi SSF47113. SSF47113. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Lung, Placenta and Spleen.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiCENPT_MOUSE
    AccessioniPrimary (citable) accession number: Q3TJM4
    Secondary accession number(s): Q3TPY6
    , Q3UPD2, Q8BTH0, Q8BTP2, Q8R5E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3