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Protein

2',5'-phosphodiesterase 12

Gene

Pde12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferon (IFNs) (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei350By similarity1
Metal bindingi350Magnesium 1By similarity1
Metal bindingi495Magnesium 2By similarity1
Metal bindingi497Magnesium 2By similarity1
Active sitei560By similarity1
Active sitei598By similarity1
Metal bindingi598Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2',5'-phosphodiesterase 12 (EC:3.1.4.-)
Short name:
2'-PDE
Short name:
2-PDE
Alternative name(s):
Mitochondrial deadenylase (EC:3.1.13.4)
Gene namesi
Name:Pde12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2443226. Pde12.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionBy similarityAdd BLAST16
ChainiPRO_000032431317 – 6082',5'-phosphodiesterase 12Add BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3TIU4.
MaxQBiQ3TIU4.
PaxDbiQ3TIU4.
PeptideAtlasiQ3TIU4.
PRIDEiQ3TIU4.

PTM databases

iPTMnetiQ3TIU4.
PhosphoSitePlusiQ3TIU4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000043702.
GenevisibleiQ3TIU4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059666.

Structurei

3D structure databases

ProteinModelPortaliQ3TIU4.
SMRiQ3TIU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000006935.
HOVERGENiHBG061027.
InParanoidiQ3TIU4.
KOiK19612.
OMAiYPSAQEK.
OrthoDBiEOG091G097K.
PhylomeDBiQ3TIU4.
TreeFamiTF323175.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3TIU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWRLPGRSAL RGVRSVVERR SRAEAGTHEA VRAMERAVVR CVPSEPKLSL
60 70 80 90 100
SFALADGSHK NMQRDQSEPL GRALSRIATN ALKGHAKVAA AKKSRKNRAH
110 120 130 140 150
SSGGAACEAT GPEPVATCEP VVKLYYREEA VAEDVLNVDA WQDGAVLQIG
160 170 180 190 200
DVKYKVERNP PTFTELQLPR YIMAGFPVCP KLGVEFGDPA SSVFRWYKEV
210 220 230 240 250
KPGAAEPGDS GPASSSHSSQ PSAWIETGVD ERVYTPCNAD IGLRLRLHCT
260 270 280 290 300
PGNGQRFGPS RELESLCPVE AGPGTCTFDH RHLYTKKVTE DSFIRTVSYN
310 320 330 340 350
ILADTYAQTE FSRTVLYPYC APYALELDYR QNLIQKELTG YNADLICLQE
360 370 380 390 400
VDRAVFSDSL VPALEAFGLE GVFRIKQHEG LATFYRKSKF RLLSQHDISF
410 420 430 440 450
QEALKSDPLH KELLEKLALN PLAQEKVLQR SSVLQISVLQ STTDSSKKIC
460 470 480 490 500
VANTHLYWHP KGGYIRLIQM EVALVHIRHV SRDLYPGIPV IFCGDFNSTP
510 520 530 540 550
STGMYHFVIS GSIAEDHEDW ASNGEEERCS MPLSHCFKLK SACGEPAYTN
560 570 580 590 600
YVGGFHGCLD YIFIDLNTLE VEQVIPLPSH EEVTTHQALP SVSHPSDHIA

LVCDLKWK
Length:608
Mass (Da):67,514
Last modified:March 18, 2008 - v2
Checksum:iDC67C45DE3FF556C
GO

Sequence cautioni

The sequence BAC34827 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21 – 22SR → TG in BAC38703 (PubMed:16141072).Curated2
Sequence conflicti104G → S in BAC40606 (PubMed:16141072).Curated1
Sequence conflicti104G → S in BAE33039 (PubMed:16141072).Curated1
Sequence conflicti123K → E in BAE39752 (PubMed:16141072).Curated1
Sequence conflicti471E → A in BAC40606 (PubMed:16141072).Curated1
Sequence conflicti471E → A in BAE33039 (PubMed:16141072).Curated1
Sequence conflicti493C → G in BAE36189 (PubMed:16141072).Curated1
Sequence conflicti523N → H in BAE36189 (PubMed:16141072).Curated1
Sequence conflicti538K → Q in BAE36189 (PubMed:16141072).Curated1
Sequence conflicti578P → L in BAE39752 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051991 mRNA. Translation: BAC34827.1. Different initiation.
AK082947 mRNA. Translation: BAC38703.1.
AK088843 mRNA. Translation: BAC40606.1.
AK155088 mRNA. Translation: BAE33039.1.
AK161083 mRNA. Translation: BAE36189.1.
AK167708 mRNA. Translation: BAE39752.1.
BC064450 mRNA. Translation: AAH64450.1.
CCDSiCCDS26882.1.
RefSeqiNP_848783.3. NM_178668.3.
UniGeneiMm.290758.
Mm.491244.

Genome annotation databases

EnsembliENSMUST00000052932; ENSMUSP00000059666; ENSMUSG00000043702.
GeneIDi211948.
KEGGimmu:211948.
UCSCiuc007stb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051991 mRNA. Translation: BAC34827.1. Different initiation.
AK082947 mRNA. Translation: BAC38703.1.
AK088843 mRNA. Translation: BAC40606.1.
AK155088 mRNA. Translation: BAE33039.1.
AK161083 mRNA. Translation: BAE36189.1.
AK167708 mRNA. Translation: BAE39752.1.
BC064450 mRNA. Translation: AAH64450.1.
CCDSiCCDS26882.1.
RefSeqiNP_848783.3. NM_178668.3.
UniGeneiMm.290758.
Mm.491244.

3D structure databases

ProteinModelPortaliQ3TIU4.
SMRiQ3TIU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059666.

PTM databases

iPTMnetiQ3TIU4.
PhosphoSitePlusiQ3TIU4.

Proteomic databases

EPDiQ3TIU4.
MaxQBiQ3TIU4.
PaxDbiQ3TIU4.
PeptideAtlasiQ3TIU4.
PRIDEiQ3TIU4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052932; ENSMUSP00000059666; ENSMUSG00000043702.
GeneIDi211948.
KEGGimmu:211948.
UCSCiuc007stb.1. mouse.

Organism-specific databases

CTDi201626.
MGIiMGI:2443226. Pde12.

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000006935.
HOVERGENiHBG061027.
InParanoidiQ3TIU4.
KOiK19612.
OMAiYPSAQEK.
OrthoDBiEOG091G097K.
PhylomeDBiQ3TIU4.
TreeFamiTF323175.

Miscellaneous databases

ChiTaRSiPde12. mouse.
PROiQ3TIU4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043702.
GenevisibleiQ3TIU4. MM.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPDE12_MOUSE
AccessioniPrimary (citable) accession number: Q3TIU4
Secondary accession number(s): Q3TTY4
, Q6P2L5, Q8BKH8, Q8BTS8, Q8BUQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.