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Protein

Synembryn-A

Gene

Ric8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  • basement membrane organization Source: MGI
  • cell-cell adhesion involved in gastrulation Source: MGI
  • cell migration involved in gastrulation Source: MGI
  • gastrulation Source: MGI
  • in utero embryonic development Source: MGI
  • regulation of G-protein coupled receptor protein signaling pathway Source: MGI
  • response to light stimulus Source: MGI
  • vasculature development Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Synembryn-A
Alternative name(s):
Protein Ric-8A
Gene namesi
Name:Ric8a
Synonyms:Ric8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141866. Ric8a.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). Colocalizes with RIC8A in CA2 hippocampal neurons.By similarity1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic development. Heterozygous mice exhibit impaired spatial memory and decreased anxiety.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Synembryn-APRO_0000235892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei435 – 4351PhosphoserineCombined sources
Modified residuei440 – 4401PhosphothreonineCombined sources
Modified residuei442 – 4421PhosphothreonineCombined sources
Modified residuei501 – 5011PhosphoserineBy similarity
Modified residuei522 – 5221PhosphoserineBy similarity
Modified residuei523 – 5231PhosphoserineBy similarity
Modified residuei527 – 5271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3TIR3.
MaxQBiQ3TIR3.
PaxDbiQ3TIR3.
PeptideAtlasiQ3TIR3.
PRIDEiQ3TIR3.

PTM databases

iPTMnetiQ3TIR3.
PhosphoSiteiQ3TIR3.

Expressioni

Tissue specificityi

Expressed in neurons and neurites of the CA1 and CA2 subregions of the hippocampus (at protein level). In adult brain, it is expressed in the neocortex, hippocampus and cerebellum as well as in the pineal gland and ependymal layer.2 Publications

Developmental stagei

During the early development (E9.5-E12.0) it is expressed in the developing nervous system such as the cranial ganglia, neural tube, sympathetic chain and dorsal root ganglia. Also found in the lens, vomeronasal organ and endolymphatic sac.1 Publication

Gene expression databases

BgeeiQ3TIR3.
GenevisibleiQ3TIR3. MM.

Interactioni

Subunit structurei

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Interacts (via C-terminus) with RGS14; the interaction stimulates the dissociation of the complex between RGS14 and the active GTP-bound form of GNAI1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221670. 1 interaction.
IntActiQ3TIR3. 1 interaction.
STRINGi10090.ENSMUSP00000026558.

Structurei

3D structure databases

ProteinModelPortaliQ3TIR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the synembryn family.Curated

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000236345.
HOVERGENiHBG054867.
InParanoidiQ3TIR3.
OMAiPMGMSPR.
OrthoDBiEOG72JWG4.
PhylomeDBiQ3TIR3.
TreeFamiTF314907.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3TIR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRAVADAL ETGEEDAVTE ALRSFNREHS QSFTFDDAQQ EDRKRLAKLL
60 70 80 90 100
VSVLEQGLSP KHRVTWLQTI RILSRDRSCL DSFASRQSLH ALACYADITV
110 120 130 140 150
SEEPIPQSPD MDVLLESLKC LCNLVLSSPT AQMLAAEARL VVRLAERVGL
160 170 180 190 200
YRKRSYPHEV QFFDLRLLFL LTALRTDVRQ QLFQELHGVR LLTDALELTL
210 220 230 240 250
GVAPKENPPV MLPAQETERA MEILKVLFNI TFDSVKREVD EEDAALYRYL
260 270 280 290 300
GTLLRHCVMV EAAGDRTEEF HGHTVNLLGN LPLKCLDVLL ALELHEGSLE
310 320 330 340 350
FMGVNMDVIS ALLAFLEKRL HQTHRLKECV APVLNVLTEC ARMHRPARKF
360 370 380 390 400
LKAQVLPPLR DVRTRPEVGD LLRNKLVRLM THLDTDVKRV AAEFLFVLCS
410 420 430 440 450
ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTEEYREAKA
460 470 480 490 500
SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS RHRVIQPMGM
510 520 530
SPRGHLTSLQ DAMCETMEGQ LSSDPDSDPD
Length:530
Mass (Da):59,847
Last modified:May 16, 2006 - v2
Checksum:iD23AFC0442CCC815
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871Q → R in BAE41115 (PubMed:16141072).Curated
Sequence conflicti274 – 2741T → A in BAE39783 (PubMed:16141072).Curated
Sequence conflicti449 – 4491K → E in BAE39783 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288813 mRNA. Translation: AAG10200.1.
AK031466 mRNA. Translation: BAC27418.1.
AK080192 mRNA. Translation: BAC37844.1.
AK155282 mRNA. Translation: BAE33163.1.
AK167746 mRNA. Translation: BAE39783.1.
AK169367 mRNA. Translation: BAE41115.1.
BC022917 mRNA. Translation: AAH22917.1.
CCDSiCCDS21988.1.
RefSeqiNP_444424.1. NM_053194.4.
UniGeneiMm.281916.

Genome annotation databases

EnsembliENSMUST00000026558; ENSMUSP00000026558; ENSMUSG00000025485.
GeneIDi101489.
KEGGimmu:101489.
UCSCiuc009kij.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288813 mRNA. Translation: AAG10200.1.
AK031466 mRNA. Translation: BAC27418.1.
AK080192 mRNA. Translation: BAC37844.1.
AK155282 mRNA. Translation: BAE33163.1.
AK167746 mRNA. Translation: BAE39783.1.
AK169367 mRNA. Translation: BAE41115.1.
BC022917 mRNA. Translation: AAH22917.1.
CCDSiCCDS21988.1.
RefSeqiNP_444424.1. NM_053194.4.
UniGeneiMm.281916.

3D structure databases

ProteinModelPortaliQ3TIR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221670. 1 interaction.
IntActiQ3TIR3. 1 interaction.
STRINGi10090.ENSMUSP00000026558.

PTM databases

iPTMnetiQ3TIR3.
PhosphoSiteiQ3TIR3.

Proteomic databases

EPDiQ3TIR3.
MaxQBiQ3TIR3.
PaxDbiQ3TIR3.
PeptideAtlasiQ3TIR3.
PRIDEiQ3TIR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026558; ENSMUSP00000026558; ENSMUSG00000025485.
GeneIDi101489.
KEGGimmu:101489.
UCSCiuc009kij.1. mouse.

Organism-specific databases

CTDi60626.
MGIiMGI:2141866. Ric8a.

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000236345.
HOVERGENiHBG054867.
InParanoidiQ3TIR3.
OMAiPMGMSPR.
OrthoDBiEOG72JWG4.
PhylomeDBiQ3TIR3.
TreeFamiTF314907.

Miscellaneous databases

PROiQ3TIR3.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TIR3.
GenevisibleiQ3TIR3. MM.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RIC-8 (Synembryn): a novel conserved protein that is required for Gq alpha signaling in the C. elegans nervous system."
    Miller K.G., Emerson M.D., McManus J.R., Rand J.B.
    Neuron 27:289-299(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J, DBA/2J and NOD.
    Tissue: Aorta, Testis and Vein.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Expression of ric-8 (synembryn) gene in the nervous system of developing and adult mouse."
    Tonissoo T., Meier R., Talts K., Plaas M., Karis A.
    Gene Expr. Patterns 3:591-594(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Heterozygous mice with Ric-8 mutation exhibit impaired spatial memory and decreased anxiety."
    Tonissoo T., Koks S., Meier R., Raud S., Plaas M., Vasar E., Karis A.
    Behav. Brain Res. 167:42-48(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435; THR-440 AND THR-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  8. "Activation of the regulator of G protein signaling 14-Galphai1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A."
    Vellano C.P., Shu F.J., Ramineni S., Yates C.K., Tall G.G., Hepler J.R.
    Biochemistry 50:752-762(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiRIC8A_MOUSE
AccessioniPrimary (citable) accession number: Q3TIR3
Secondary accession number(s): Q3TEY3, Q99JW0, Q9ERR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: July 6, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.