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Protein

S-adenosylmethionine synthase isoform type-2

Gene

Mat2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase isoform type-2 (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase isoform type-1 (Mat1a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29ATPBy similarity1
Metal bindingi31MagnesiumBy similarity1
Metal bindingi57PotassiumBy similarity1
Binding sitei70MethionineBy similarity1
Binding sitei113MethionineBy similarity1
Binding sitei258ATPBy similarity1
Binding sitei258Methionine; shared with neighboring subunitBy similarity1
Binding sitei281ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei285ATP; shared with neighboring subunitBy similarity1
Binding sitei289MethionineBy similarity1
Binding sitei291ATP; shared with neighboring subunitBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 181ATPBy similarity3
Nucleotide bindingi247 – 250ATPBy similarity4
Nucleotide bindingi264 – 265ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • methionine adenosyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-156581. Methylation.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase isoform type-2 (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase 2
Alternative name(s):
Methionine adenosyltransferase 2
Short name:
MAT 2
Gene namesi
Name:Mat2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2443731. Mat2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001744371 – 395S-adenosylmethionine synthase isoform type-2Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81N6-acetyllysineBy similarity1
Modified residuei114PhosphoserineBy similarity1
Modified residuei384PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3THS6.
PaxDbiQ3THS6.
PeptideAtlasiQ3THS6.
PRIDEiQ3THS6.

PTM databases

iPTMnetiQ3THS6.
PhosphoSitePlusiQ3THS6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053907.
CleanExiMM_MAT2A.
ExpressionAtlasiQ3THS6. baseline and differential.
GenevisibleiQ3THS6. MM.

Interactioni

Subunit structurei

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain.By similarity

Protein-protein interaction databases

DIPiDIP-59526N.
IntActiQ3THS6. 1 interactor.
STRINGi10090.ENSMUSP00000087118.

Structurei

3D structure databases

ProteinModelPortaliQ3THS6.
SMRiQ3THS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 125Flexible loopBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
HOVERGENiHBG001562.
InParanoidiQ3THS6.
KOiK00789.
OMAiKTIWHIN.
OrthoDBiEOG091G08Z2.
PhylomeDBiQ3THS6.
TreeFamiTF300511.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3THS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGQLNGFHE AFIEEGTFLF TSESVGEGHP DKICDQISDA VLDAHLQQDP
60 70 80 90 100
DAKVACETVA KTGMILLAGE ITSRAAIDYQ KVVREAIKHI GYDDSSKGFD
110 120 130 140 150
YKTCNVLVAL EQQSPDIAQG VHLDRNEEDI GAGDQGLMFG YATDETEECM
160 170 180 190 200
PLTIVLAHKL NAKLAELRRN GTLPWLRPDS KTQVTVQYMQ DRGAVLPIRV
210 220 230 240 250
HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF
260 270 280 290 300
VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW
310 320 330 340 350
VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK
360 370 380 390
KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY
Length:395
Mass (Da):43,689
Last modified:January 24, 2006 - v2
Checksum:iBB977989B75CB8F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24S → T in BAE40120 (PubMed:16141072).Curated1
Sequence conflicti128E → G in BAD06937 (Ref. 1) Curated1
Sequence conflicti283S → L in BAE38932 (PubMed:16141072).Curated1
Sequence conflicti302A → S in BAE40120 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070266 mRNA. Translation: BAD06937.1.
AK166671 mRNA. Translation: BAE38932.1.
AK168155 mRNA. Translation: BAE40120.1.
CCDSiCCDS39515.1.
RefSeqiNP_663544.1. NM_145569.4.
UniGeneiMm.29815.
Mm.440743.
Mm.485037.

Genome annotation databases

EnsembliENSMUST00000059472; ENSMUSP00000087118; ENSMUSG00000053907.
ENSMUST00000193334; ENSMUSP00000142136; ENSMUSG00000104463.
GeneIDi232087.
KEGGimmu:232087.
UCSCiuc009cip.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070266 mRNA. Translation: BAD06937.1.
AK166671 mRNA. Translation: BAE38932.1.
AK168155 mRNA. Translation: BAE40120.1.
CCDSiCCDS39515.1.
RefSeqiNP_663544.1. NM_145569.4.
UniGeneiMm.29815.
Mm.440743.
Mm.485037.

3D structure databases

ProteinModelPortaliQ3THS6.
SMRiQ3THS6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59526N.
IntActiQ3THS6. 1 interactor.
STRINGi10090.ENSMUSP00000087118.

PTM databases

iPTMnetiQ3THS6.
PhosphoSitePlusiQ3THS6.

Proteomic databases

EPDiQ3THS6.
PaxDbiQ3THS6.
PeptideAtlasiQ3THS6.
PRIDEiQ3THS6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059472; ENSMUSP00000087118; ENSMUSG00000053907.
ENSMUST00000193334; ENSMUSP00000142136; ENSMUSG00000104463.
GeneIDi232087.
KEGGimmu:232087.
UCSCiuc009cip.1. mouse.

Organism-specific databases

CTDi4144.
MGIiMGI:2443731. Mat2a.

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
HOVERGENiHBG001562.
InParanoidiQ3THS6.
KOiK00789.
OMAiKTIWHIN.
OrthoDBiEOG091G08Z2.
PhylomeDBiQ3THS6.
TreeFamiTF300511.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
ReactomeiR-MMU-156581. Methylation.

Miscellaneous databases

ChiTaRSiMat2a. mouse.
PROiQ3THS6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053907.
CleanExiMM_MAT2A.
ExpressionAtlasiQ3THS6. baseline and differential.
GenevisibleiQ3THS6. MM.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETK2_MOUSE
AccessioniPrimary (citable) accession number: Q3THS6
Secondary accession number(s): Q3TL60, Q76LX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.