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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

Gmps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (Gmps)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104For GATase activityPROSITE-ProRule annotation1
Active sitei190For GATase activityPROSITE-ProRule annotation1
Active sitei192For GATase activityPROSITE-ProRule annotation1
Binding sitei337SubstrateBy similarity1
Binding sitei522SubstrateBy similarity1
Binding sitei610SubstrateBy similarity1
Binding sitei685SubstrateBy similarity1
Binding sitei691SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi244 – 250ATPPROSITE-ProRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.950.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:Gmps
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2448526. Gmps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2765.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002843652 – 693GMP synthase [glutamine-hydrolyzing]Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei9N6-acetyllysineBy similarity1
Modified residuei318PhosphothreonineBy similarity1
Modified residuei332PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3THK7.
MaxQBiQ3THK7.
PaxDbiQ3THK7.
PRIDEiQ3THK7.

2D gel databases

REPRODUCTION-2DPAGEIPI00351252.

PTM databases

iPTMnetiQ3THK7.
PhosphoSitePlusiQ3THK7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027823.
CleanExiMM_GMPS.
ExpressionAtlasiQ3THK7. baseline and differential.
GenevisibleiQ3THK7. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi230837. 2 interactors.
STRINGi10090.ENSMUSP00000029405.

Chemistry databases

BindingDBiQ3THK7.

Structurei

3D structure databases

ProteinModelPortaliQ3THK7.
SMRiQ3THK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 216Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST190
Domaini217 – 435GMPS ATP-PPasePROSITE-ProRule annotationAdd BLAST219

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation
Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1622. Eukaryota.
COG0518. LUCA.
COG0519. LUCA.
GeneTreeiENSGT00390000006591.
HOGENOMiHOG000223965.
HOVERGENiHBG005929.
InParanoidiQ3THK7.
KOiK01951.
OMAiNMTTNPE.
OrthoDBiEOG091G031G.
PhylomeDBiQ3THK7.
TreeFamiTF106132.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3THK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALCNGDSKP ENAGGDLKDG SHHYEGAVVI LDAGAQYGKV IDRRVRELFV
60 70 80 90 100
QSEIFPLETP AFAIKEQGFR AIIISGGPNS VYAEDAPWFD PAIFTIGKPI
110 120 130 140 150
LGICYGMQMM NKVFGGTVHK KSVREDGVFN ISMDNTCSLF RGLQKEEIVL
160 170 180 190 200
LTHGDSVDKV ADGFKVVARS GNIVAGIANE SKKLYGVQFH PEVGLTENGK
210 220 230 240 250
VILKNFLYDI AGCSGNFTVQ NRELECIREI KEKVGTSKVL VLLSGGVDST
260 270 280 290 300
VCTALLNRAL NQDQVIAVHI DNGFMRKRES QSVEEALKKL GIQVKVINAA
310 320 330 340 350
HSFYNGTTTL PISDEDRTPR KRISKTLNMT TSPEEKRKII GDTFVKIANE
360 370 380 390 400
VIGEMSLKPE EVFLAQGTLR PDLIESASLV ASGKAELIKT HHNDTELIRK
410 420 430 440 450
LREEGKVIEP LKDFHKDEVR ILGRELDLPE ELVSRHPFPG PGLAIRVICA
460 470 480 490 500
EEPYICKDFP ETNNILKIVA DFSASVKKPH TLLQRVKACT TEEDQEKLMQ
510 520 530 540 550
ITSLHSLNAF LLPIKTVGVQ GDCRSYSYVC GISSKDEPDW ESLIFLARLI
560 570 580 590 600
PRMCHNINRV VYIFGPPVKE PPTDVTPTFL TTGVLSTLRQ ADFEAHNILR
610 620 630 640 650
ESGFAGKISQ MPVILTPLHF DRDPLQKQPS CQRSVVIRTF ITSDFMTGVP
660 670 680 690
ATPGNEIPVE VVLKMVTEIK KIPGISRIMY DLTSKPPGTT EWE
Length:693
Mass (Da):76,723
Last modified:April 17, 2007 - v2
Checksum:i854DBAD6A9C75A12
GO

Sequence cautioni

The sequence BAE20667 differs from that shown. Reason: Frameshift at position 35.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201V → I in BAE39875 (PubMed:16141072).Curated1
Sequence conflicti205N → Y in BAE39875 (PubMed:16141072).Curated1
Sequence conflicti265V → D in BAE39875 (PubMed:16141072).Curated1
Sequence conflicti356S → G in BAE40189 (PubMed:16141072).Curated1
Sequence conflicti359P → H in BAE40189 (PubMed:16141072).Curated1
Sequence conflicti359P → R in BAE39875 (PubMed:16141072).Curated1
Sequence conflicti363Missing in BAE39875 (PubMed:16141072).Curated1
Sequence conflicti511 – 512LL → CC in BAE27334 (PubMed:16141072).Curated2
Sequence conflicti586S → G in BAE39875 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049755 mRNA. Translation: BAE20667.1. Frameshift.
AK143197 mRNA. Translation: BAE25299.1.
AK146654 mRNA. Translation: BAE27334.1.
AK167857 mRNA. Translation: BAE39875.1.
AK168239 mRNA. Translation: BAE40189.1.
AK169043 mRNA. Translation: BAE40832.1.
AK169701 mRNA. Translation: BAE41314.1.
BC080685 mRNA. Translation: AAH80685.1.
CCDSiCCDS17383.1.
RefSeqiNP_001028472.2. NM_001033300.2.
UniGeneiMm.331051.
Mm.394565.
Mm.490620.

Genome annotation databases

EnsembliENSMUST00000029405; ENSMUSP00000029405; ENSMUSG00000027823.
GeneIDi229363.
KEGGimmu:229363.
UCSCiuc008pke.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049755 mRNA. Translation: BAE20667.1. Frameshift.
AK143197 mRNA. Translation: BAE25299.1.
AK146654 mRNA. Translation: BAE27334.1.
AK167857 mRNA. Translation: BAE39875.1.
AK168239 mRNA. Translation: BAE40189.1.
AK169043 mRNA. Translation: BAE40832.1.
AK169701 mRNA. Translation: BAE41314.1.
BC080685 mRNA. Translation: AAH80685.1.
CCDSiCCDS17383.1.
RefSeqiNP_001028472.2. NM_001033300.2.
UniGeneiMm.331051.
Mm.394565.
Mm.490620.

3D structure databases

ProteinModelPortaliQ3THK7.
SMRiQ3THK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230837. 2 interactors.
STRINGi10090.ENSMUSP00000029405.

Chemistry databases

BindingDBiQ3THK7.
ChEMBLiCHEMBL2765.

Protein family/group databases

MEROPSiC26.950.

PTM databases

iPTMnetiQ3THK7.
PhosphoSitePlusiQ3THK7.

2D gel databases

REPRODUCTION-2DPAGEIPI00351252.

Proteomic databases

EPDiQ3THK7.
MaxQBiQ3THK7.
PaxDbiQ3THK7.
PRIDEiQ3THK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029405; ENSMUSP00000029405; ENSMUSG00000027823.
GeneIDi229363.
KEGGimmu:229363.
UCSCiuc008pke.1. mouse.

Organism-specific databases

CTDi8833.
MGIiMGI:2448526. Gmps.

Phylogenomic databases

eggNOGiKOG1622. Eukaryota.
COG0518. LUCA.
COG0519. LUCA.
GeneTreeiENSGT00390000006591.
HOGENOMiHOG000223965.
HOVERGENiHBG005929.
InParanoidiQ3THK7.
KOiK01951.
OMAiNMTTNPE.
OrthoDBiEOG091G031G.
PhylomeDBiQ3THK7.
TreeFamiTF106132.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ3THK7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027823.
CleanExiMM_GMPS.
ExpressionAtlasiQ3THK7. baseline and differential.
GenevisibleiQ3THK7. MM.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_MOUSE
AccessioniPrimary (citable) accession number: Q3THK7
Secondary accession number(s): Q3TFR6
, Q3TIH1, Q3UJ21, Q3V343, Q66JZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.