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Protein

General transcription factor IIF subunit 1

Gene

Gtf2f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 1
Alternative name(s):
Transcription initiation factor IIF subunit alpha
Short name:
TFIIF-alpha
Gene namesi
Name:Gtf2f1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1923848 Gtf2f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002603222 – 508General transcription factor IIF subunit 1Add BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei156PhosphothreonineBy similarity1
Modified residuei217PhosphoserineCombined sources1
Modified residuei218PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei331PhosphothreonineBy similarity1
Modified residuei377PhosphoserineBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei381PhosphoserineBy similarity1
Modified residuei385PhosphoserineBy similarity1
Modified residuei389PhosphothreonineCombined sources1
Modified residuei391PhosphoserineBy similarity1
Modified residuei407N6-acetyllysineCombined sources1
Modified residuei431PhosphoserineBy similarity1
Modified residuei433PhosphoserineCombined sources1
Modified residuei436PhosphoserineBy similarity1
Modified residuei437PhosphothreonineBy similarity1
Modified residuei440PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3THK3
MaxQBiQ3THK3
PaxDbiQ3THK3
PeptideAtlasiQ3THK3
PRIDEiQ3THK3

PTM databases

iPTMnetiQ3THK3
PhosphoSitePlusiQ3THK3

Expressioni

Gene expression databases

BgeeiENSMUSG00000002658
CleanExiMM_GTF2F1
GenevisibleiQ3THK3 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220965, 1 interactor
CORUMiQ3THK3
IntActiQ3THK3, 1 interactor
MINTiQ3THK3
STRINGi10090.ENSMUSP00000002733

Structurei

3D structure databases

ProteinModelPortaliQ3THK3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi283 – 350Glu-richAdd BLAST68

Sequence similaritiesi

Belongs to the TFIIF alpha subunit family.Curated

Phylogenomic databases

eggNOGiKOG2393 Eukaryota
ENOG410XSRW LUCA
GeneTreeiENSGT00440000038032
HOGENOMiHOG000059644
HOVERGENiHBG000729
InParanoidiQ3THK3
KOiK03138
OMAiGAIEAYP
OrthoDBiEOG091G0N3J
TreeFamiTF313850

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR008851 TFIIF-alpha
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF05793 TFIIF_alpha, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3THK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALGSSSQN VTEYVVRVPK NTAKRYNIMA FNAADKVNFA TWNQARLERD
60 70 80 90 100
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL
110 120 130 140 150
RVNGKSGRKF KGIKKGGVTE NTAYYIFTQC ADGAFEAFPV QNWYNFTPLA
160 170 180 190 200
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRSRK
210 220 230 240 250
KPSELRIHDL EDDLEMSSDA SDASGEEGSR TSKAKKKAPV TKAGRKKKKK
260 270 280 290 300
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SPDETEGKPK VPQQEDGPKG
310 320 330 340 350
VDEQSESSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSDDSDSSEE
360 370 380 390 400
SDIDSETSSA LFMAKKKTPP KRERKPSGGS SKGTSRPGTP SAEAASTSST
410 420 430 440 450
LRAAASKLEQ GKRTSETPAA KRLRMDTGPQ SLSGKSTPSS GDVQVTEDAV
460 470 480 490 500
RRYLTRKPMT TKDLLKKFQT KKTGLSSEQT VNVLAQILKR LNPERKMIGD

KMHFSLKE
Length:508
Mass (Da):57,241
Last modified:July 27, 2011 - v2
Checksum:iEB6DDD074CFBA712
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139P → T in BAE40186 (PubMed:16141072).Curated1
Sequence conflicti139P → T in BAE40193 (PubMed:16141072).Curated1
Sequence conflicti185Q → E in BAE40186 (PubMed:16141072).Curated1
Sequence conflicti185Q → E in BAE40193 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK168236 mRNA Translation: BAE40186.1
AK168243 mRNA Translation: BAE40193.1
CH466537 Genomic DNA Translation: EDL38221.1
BC023081 mRNA Translation: AAH23081.1
BC031123 mRNA Translation: AAH31123.1
CCDSiCCDS37667.1
RefSeqiNP_598562.1, NM_133801.2
UniGeneiMm.24632

Genome annotation databases

EnsembliENSMUST00000002733; ENSMUSP00000002733; ENSMUSG00000002658
GeneIDi98053
KEGGimmu:98053
UCSCiuc008ddp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiT2FA_MOUSE
AccessioniPrimary (citable) accession number: Q3THK3
Secondary accession number(s): Q8R5B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health