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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Gene

Stt3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of oligosaccharyltransferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation (By similarity).By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit STT3B
Short name:
STT3-B
Alternative name(s):
B6dom1 antigen
Source of immunodominant MHC-associated peptides
Gene namesi
Name:Stt3b
Synonyms:Simp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915542. Stt3b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Transmembranei166 – 186HelicalSequence analysisAdd BLAST21
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Transmembranei240 – 256HelicalSequence analysisAdd BLAST17
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Transmembranei461 – 481HelicalSequence analysisAdd BLAST21
Transmembranei535 – 555HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002460022 – 823Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3BAdd BLAST822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei13PhosphoserineCombined sources1
Modified residuei18PhosphoserineBy similarity1
Modified residuei29PhosphoserineCombined sources1
Modified residuei495PhosphoserineCombined sources1
Modified residuei496PhosphoserineCombined sources1
Glycosylationi624N-linked (GlcNAc...)1 Publication1
Glycosylationi638N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ3TDQ1.
MaxQBiQ3TDQ1.
PaxDbiQ3TDQ1.
PeptideAtlasiQ3TDQ1.
PRIDEiQ3TDQ1.

PTM databases

iPTMnetiQ3TDQ1.
PhosphoSitePlusiQ3TDQ1.
SwissPalmiQ3TDQ1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032437.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi212788. 2 interactors.
IntActiQ3TDQ1. 3 interactors.
MINTiMINT-3251506.
STRINGi10090.ENSMUSP00000035010.

Structurei

3D structure databases

ProteinModelPortaliQ3TDQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
HOVERGENiHBG010606.
InParanoidiQ3TDQ1.
KOiK07151.
PhylomeDBiQ3TDQ1.
TreeFamiTF300822.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3TDQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPSAPESK HKSSLNSSPW SGLMALGNSR HGHHGPGTQS ASSAAAPKPG
60 70 80 90 100
PPAGLSGGLS QPAGWQSLLS FTILFLAWLA GFSSRLFAVI RFESIIHEFD
110 120 130 140 150
PWFNYRSTHH LASHGFYEFL NWFDERAWYP LGRIVGGTVY PGLMITAGLI
160 170 180 190 200
HWILNTLNIT VHIRDVCVFL APTFSGLTSI STFLLTRELW NQGAGLLAAC
210 220 230 240 250
FIAIVPGYIS RSVAGSFDNE GIAIFALQFT YYLWVKSVKT GSVFWTMCCC
260 270 280 290 300
LSYFYMVSAW GGYVFIINLI PLHVFVLLLM QRYSKRVYIA YSTFYIVGLI
310 320 330 340 350
LSMQIPFVGF QPIRTSEHMA AAGVFALLQA YAFLQYLRDR LTKQEFQTLF
360 370 380 390 400
FLGVSLAAGA VFLSVIYLTY TGYIAPWSGR FYSLWDTGYA KIHIPIIASV
410 420 430 440 450
SEHQPTTWVS FFFDLHILVC TFPAGLWFCI KNINDERVFV ALYAISAVYF
460 470 480 490 500
AGVMVRLMLT LTPVVCMLSA IAFSNVFEHY LGDDMKRENP PVEDSSDEDD
510 520 530 540 550
KRNPGNLYDK AGKVRKHVTE QEKPEEGLGP NIKSIVTMLM LMLLMMFAVH
560 570 580 590 600
CTWVTSNAYS SPSVVLASYN HDGTRNILDD FREAYFWLRQ NTDEHARVMS
610 620 630 640 650
WWDYGYQIAG MANRTTLVDN NTWNNSHIAL VGKAMSSNET AAYKIMRSLD
660 670 680 690 700
VDYVLVIFGG VIGYSGDDIN KFLWMVRIAE GEHPKDIREG DYFTQQGEFR
710 720 730 740 750
VDKAGSPTLL NCLMYKMSYY RFGEMQLDFR TPPGFDRTRN AEIGNKDIKF
760 770 780 790 800
KHLEEAFTSE HWLVRIYKVK APDNRETLGH KPRVTNIVPK QKYLSKKTTK
810 820
RKRGYVKNKL VFKKGKKTSK KTV
Length:823
Mass (Da):93,246
Last modified:July 11, 2006 - v2
Checksum:iBBC27DB07EE609D1
GO

Sequence cautioni

The sequence AAH13054 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43S → R in BAE41550 (PubMed:16141072).Curated1
Sequence conflicti43S → R in AAH03206 (PubMed:15489334).Curated1
Sequence conflicti240T → I in BAE41550 (PubMed:16141072).Curated1
Sequence conflicti294F → L in BAB31390 (PubMed:16141072).Curated1

Polymorphismi

In strains 129, C57BL/10, C57BL/6 and LP Glu-776 correlates with a B6dom1-positive phenotype, Asp-776 is found in resistant strains. The B6dom1 minor histocompatibilty antigen (MiHA) is used as a model antigen in studying immunodominance.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti776E → D in strain: A.BY, B10.H7 and C3H.SW; correlated with B6dom1-negative phenotype. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018758 mRNA. Translation: BAB31390.1.
AK145674 mRNA. Translation: BAE26582.1.
AK154979 mRNA. Translation: BAE32968.1.
AK152899 mRNA. Translation: BAE31580.1.
AK170079 mRNA. Translation: BAE41550.1.
BC003206 mRNA. Translation: AAH03206.1.
BC013054 mRNA. Translation: AAH13054.2. Different initiation.
BC052433 mRNA. Translation: AAH52433.1.
CCDSiCCDS23599.1.
RefSeqiNP_077184.2. NM_024222.2.
UniGeneiMm.296158.

Genome annotation databases

GeneIDi68292.
KEGGimmu:68292.
UCSCiuc009ryp.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018758 mRNA. Translation: BAB31390.1.
AK145674 mRNA. Translation: BAE26582.1.
AK154979 mRNA. Translation: BAE32968.1.
AK152899 mRNA. Translation: BAE31580.1.
AK170079 mRNA. Translation: BAE41550.1.
BC003206 mRNA. Translation: AAH03206.1.
BC013054 mRNA. Translation: AAH13054.2. Different initiation.
BC052433 mRNA. Translation: AAH52433.1.
CCDSiCCDS23599.1.
RefSeqiNP_077184.2. NM_024222.2.
UniGeneiMm.296158.

3D structure databases

ProteinModelPortaliQ3TDQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212788. 2 interactors.
IntActiQ3TDQ1. 3 interactors.
MINTiMINT-3251506.
STRINGi10090.ENSMUSP00000035010.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

PTM databases

iPTMnetiQ3TDQ1.
PhosphoSitePlusiQ3TDQ1.
SwissPalmiQ3TDQ1.

Proteomic databases

EPDiQ3TDQ1.
MaxQBiQ3TDQ1.
PaxDbiQ3TDQ1.
PeptideAtlasiQ3TDQ1.
PRIDEiQ3TDQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi68292.
KEGGimmu:68292.
UCSCiuc009ryp.1. mouse.

Organism-specific databases

CTDi201595.
MGIiMGI:1915542. Stt3b.

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
HOVERGENiHBG010606.
InParanoidiQ3TDQ1.
KOiK07151.
PhylomeDBiQ3TDQ1.
TreeFamiTF300822.

Miscellaneous databases

ChiTaRSiStt3b. mouse.
PROiQ3TDQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032437.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTT3B_MOUSE
AccessioniPrimary (citable) accession number: Q3TDQ1
Secondary accession number(s): Q7TT24
, Q921E3, Q99LL0, Q9D2V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.