Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FAS-associated factor 2

Gene

Faf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
FAS-associated factor 2
Alternative name(s):
UBX domain-containing protein 8
Gene namesi
Name:Faf2
Synonyms:Kiaa0887, Ubxd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1923827. Faf2.

Subcellular locationi

  • Cytoplasm By similarity
  • Lipid droplet By similarity
  • Endoplasmic reticulum By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Lipid droplet

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 445444FAS-associated factor 2PRO_0000244065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei167 – 1671N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ3TDN2.
MaxQBiQ3TDN2.
PaxDbiQ3TDN2.
PeptideAtlasiQ3TDN2.
PRIDEiQ3TDN2.

PTM databases

iPTMnetiQ3TDN2.
PhosphoSiteiQ3TDN2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025873.
ExpressionAtlasiQ3TDN2. baseline and differential.
GenevisibleiQ3TDN2. MM.

Interactioni

Subunit structurei

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with YOD1. Interacts (via N-terminus) with UBQLN2 (via C-terminus). Interacts with PNPLA2 and UBAC2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000121182.

Structurei

3D structure databases

ProteinModelPortaliQ3TDN2.
SMRiQ3TDN2. Positions 2-56, 136-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 4837UBAAdd
BLAST
Domaini357 – 43983UBXPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili275 – 35076Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 UBA domain.Curated
Contains 1 UBX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1363. Eukaryota.
ENOG410XSFS. LUCA.
GeneTreeiENSGT00530000063422.
HOGENOMiHOG000007539.
HOVERGENiHBG101311.
InParanoidiQ3TDN2.
KOiK18726.
OMAiPCLPTEE.
OrthoDBiEOG091G07IN.
PhylomeDBiQ3TDN2.
TreeFamiTF314172.

Family and domain databases

InterProiIPR012336. Thioredoxin-like_fold.
IPR006577. UAS.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00594. UAS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TDN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPEEQDLT QEQTEKLLQF QDLTGIESME QCRLALEQHN WNMEAAVQDR
60 70 80 90 100
LNEQEGVPSV FNPPPARPLQ VNTADHRIYS YVVSRPQPRG LLGWGYYLIM
110 120 130 140 150
LPFRFTYYTI LDIFRFALRF IRPDPRSRVT DPVGDIVSFM HSFEEKYGRA
160 170 180 190 200
HPVFYQGTYS QALNDAKREL RFLLVYLHGD DHQDSDEFCR NALCAPEVIS
210 220 230 240 250
LINSRMLFWA CSTNKPEGYR VSQALRENTY PFLAMIMLKD RRMTVVGRLE
260 270 280 290 300
GLIQPDDLIN QLTFIMDANQ TYLVSERLER EERNQTQVLR QQQDEAYLAS
310 320 330 340 350
LRADQEKERK KREEKERKRR KEEEVQQQKL AEERRRQNLQ EEKERKLECL
360 370 380 390 400
PPEPSPDDPE SVKIIFKLPN DSRVERRFHF SQSLTVIHDF LFSLKESPEK
410 420 430 440
FQIEANFPRR VLPCVPSEEW PNPPTLQEAG LSHTEVLFVQ DLTDE
Length:445
Mass (Da):52,471
Last modified:June 27, 2006 - v2
Checksum:iF0FD0FC631148AE7
GO
Isoform 2 (identifier: Q3TDN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-89: Missing.

Note: No experimental confirmation available.
Show »
Length:426
Mass (Da):50,231
Checksum:iEFA71B09CB52A7D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721Missing in AAH46817 (PubMed:15489334).Curated
Sequence conflicti430 – 4345GLSHT → DSATQ in BAE41569 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei71 – 8919Missing in isoform 2. 1 PublicationVSP_019504Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151617 mRNA. Translation: BAE30554.1.
AK170111 mRNA. Translation: BAE41569.1.
BC046817 mRNA. Translation: AAH46817.1.
AK122394 mRNA. Translation: BAC65676.1.
CCDSiCCDS36668.1. [Q3TDN2-1]
RefSeqiNP_848484.2. NM_178397.3. [Q3TDN2-1]
XP_011242883.1. XM_011244581.1. [Q3TDN2-2]
UniGeneiMm.194459.

Genome annotation databases

EnsembliENSMUST00000026991; ENSMUSP00000026991; ENSMUSG00000025873. [Q3TDN2-2]
ENSMUST00000126071; ENSMUSP00000121182; ENSMUSG00000025873. [Q3TDN2-1]
GeneIDi76577.
KEGGimmu:76577.
UCSCiuc007qoq.2. mouse. [Q3TDN2-1]
uc007qor.2. mouse. [Q3TDN2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151617 mRNA. Translation: BAE30554.1.
AK170111 mRNA. Translation: BAE41569.1.
BC046817 mRNA. Translation: AAH46817.1.
AK122394 mRNA. Translation: BAC65676.1.
CCDSiCCDS36668.1. [Q3TDN2-1]
RefSeqiNP_848484.2. NM_178397.3. [Q3TDN2-1]
XP_011242883.1. XM_011244581.1. [Q3TDN2-2]
UniGeneiMm.194459.

3D structure databases

ProteinModelPortaliQ3TDN2.
SMRiQ3TDN2. Positions 2-56, 136-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000121182.

PTM databases

iPTMnetiQ3TDN2.
PhosphoSiteiQ3TDN2.

Proteomic databases

EPDiQ3TDN2.
MaxQBiQ3TDN2.
PaxDbiQ3TDN2.
PeptideAtlasiQ3TDN2.
PRIDEiQ3TDN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026991; ENSMUSP00000026991; ENSMUSG00000025873. [Q3TDN2-2]
ENSMUST00000126071; ENSMUSP00000121182; ENSMUSG00000025873. [Q3TDN2-1]
GeneIDi76577.
KEGGimmu:76577.
UCSCiuc007qoq.2. mouse. [Q3TDN2-1]
uc007qor.2. mouse. [Q3TDN2-2]

Organism-specific databases

CTDi23197.
MGIiMGI:1923827. Faf2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1363. Eukaryota.
ENOG410XSFS. LUCA.
GeneTreeiENSGT00530000063422.
HOGENOMiHOG000007539.
HOVERGENiHBG101311.
InParanoidiQ3TDN2.
KOiK18726.
OMAiPCLPTEE.
OrthoDBiEOG091G07IN.
PhylomeDBiQ3TDN2.
TreeFamiTF314172.

Miscellaneous databases

PROiQ3TDN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025873.
ExpressionAtlasiQ3TDN2. baseline and differential.
GenevisibleiQ3TDN2. MM.

Family and domain databases

InterProiIPR012336. Thioredoxin-like_fold.
IPR006577. UAS.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00594. UAS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFAF2_MOUSE
AccessioniPrimary (citable) accession number: Q3TDN2
Secondary accession number(s): Q3U9W2, Q80TP7, Q80W42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.