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Protein

Gamma-secretase-activating protein

Gene

Gsap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta-amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD.1 Publication

GO - Molecular functioni

GO - Biological processi

  • positive regulation of beta-amyloid formation Source: UniProtKB
  • regulation of proteolysis Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-secretase-activating protein
Short name:
GSAP
Alternative name(s):
Protein pigeon homolog
Cleaved into the following chain:
Gene namesi
Name:Gsap
Synonyms:Pion
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2442259. Gsap.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Gamma-secretase-activating proteinPRO_0000335810Add
BLAST
Chaini738 – 858121Gamma-secretase-activating protein 16 kDa C-terminal formSequence analysisPRO_0000403729Add
BLAST

Post-translational modificationi

The protein is first synthesized as a holoprotein form of 98 kDa and rapidly processed into the gamma-secretase-activating protein 16 kDa C-terminal form, which constitutes the predominant form.By similarity

Proteomic databases

MaxQBiQ3TCV3.
PaxDbiQ3TCV3.
PRIDEiQ3TCV3.

PTM databases

iPTMnetiQ3TCV3.
PhosphoSiteiQ3TCV3.

Expressioni

Gene expression databases

BgeeiQ3TCV3.
ExpressionAtlasiQ3TCV3. baseline and differential.
GenevisibleiQ3TCV3. MM.

Interactioni

Subunit structurei

Interacts with APP; specifically interacts with the CTF-alpha product of APP. Interacts with the gamma-secretase complex (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-59239N.
STRINGi10090.ENSMUSP00000043679.

Family & Domainsi

Sequence similaritiesi

Belongs to the GSAP family.Curated

Phylogenomic databases

eggNOGiENOG410IG5Q. Eukaryota.
ENOG41129DZ. LUCA.
GeneTreeiENSGT00390000012875.
HOVERGENiHBG095500.
InParanoidiQ3TCV3.
OMAiSATRTCW.
OrthoDBiEOG767391.
PhylomeDBiQ3TCV3.
TreeFamiTF323853.

Family and domain databases

InterProiIPR028010. GSAP_C_dom.
IPR026172. GSAP_fam.
[Graphical view]
PANTHERiPTHR13630. PTHR13630. 1 hit.
PfamiPF14959. GSAP-16. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TCV3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRLVTHFD VLEDVLPSLL TQAATTDEGD RAGVLETTYG SLRVLNIERN
60 70 80 90 100
GNIIYTYKDN KGNAVFGLYD CQTRQNEHLY TFEKDMQAVS CSVNSERTVL
110 120 130 140 150
AASFIQYTTE GVKNDLQPGS KCLTLLVEIH PVNNVKVLKA VDSCVWVQFL
160 170 180 190 200
YPQAESHLLP QNHLLLISEE KYIERFHIQI TREDGDRVVI RNSSHLPRDR
210 220 230 240 250
LAEDFVWAQW DLSEQRLYYI ELKESRSILK CIQFRADESF NLMFEMPLDI
260 270 280 290 300
TLTGLRFKLV NFGYDYRQDR EKLCNQPSLC IFTNHTGSLC MCYSPKSDSR
310 320 330 340 350
EEITYSVFYL HKGYRKIFTA APGSADSQVT NGADSQVTDG IAFLNLGYFV
360 370 380 390 400
AVYSPGHFLH LLNIQHPDLV CHSLFLTGNN KIAAVLPPSP LQSLPGSLVL
410 420 430 440 450
DCYSGKVYRV TLDQSYLLRF LWNAHLDCER MAALHCILSC SQDPGFPEEQ
460 470 480 490 500
IIQWISEHVS ACHSFDLIQE FLIASSYWSV YAELDDMGML LQYSSVLTWN
510 520 530 540 550
TEIPGIKFTT EELPLPLMKV YGLKGYWAKL NSNLEYIKYT KPHLHYHNSV
560 570 580 590 600
VRREWHNLIS EERTGKRRST MYVRNILENA MKVIASMETR TLEPRLIPFL
610 620 630 640 650
QEEDRHQRLL MGLMVSELRD HLLRHLQGVE KKKIEQMVLD YISKLLDLIW
660 670 680 690 700
CLLETSWRKH SMHPLVLHLN SHCSAADFEV FHLMTRILDA ASSLCLPLPP
710 720 730 740 750
GFHSLHTILG VHCLPLYSLL HYIDNGVLLL TETAVTRLMK DLDNSEKNEQ
760 770 780 790 800
LKFSIIVRLP PLIGQKVCRL WDHPMSSNII SRNHVARLLK NYRKEPRNSM
810 820 830 840 850
IDKSSFPVEF LPLNYFIEIL MGLESSNQAL YGFEGHDNVD AEFVEEAALK

HTTMLLGL
Length:858
Mass (Da):98,694
Last modified:October 11, 2005 - v1
Checksum:i326E86B9E35A555B
GO
Isoform 2 (identifier: Q3TCV3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     521-526: YGLKGY → SPAPRG
     527-858: Missing.

Show »
Length:526
Mass (Da):59,870
Checksum:iCE70C4CA7B6AD352
GO
Isoform 3 (identifier: Q3TCV3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     172-172: Y → F
     173-858: Missing.

Show »
Length:172
Mass (Da):19,351
Checksum:i3784B21964E0761D
GO

Sequence cautioni

The sequence AAH22737.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC28433.1 differs from that shown. Reason: Frameshift at positions 413 and 513. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti392 – 3921Q → R in BAC37858 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei172 – 1721Y → F in isoform 3. 1 PublicationVSP_033775
Alternative sequencei173 – 858686Missing in isoform 3. 1 PublicationVSP_033776Add
BLAST
Alternative sequencei521 – 5266YGLKGY → SPAPRG in isoform 2. 1 PublicationVSP_033777
Alternative sequencei527 – 858332Missing in isoform 2. 1 PublicationVSP_033778Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033694 mRNA. Translation: BAC28433.1. Frameshift.
AK080244 mRNA. Translation: BAC37858.1.
AK137494 mRNA. Translation: BAE23378.1.
AK170515 mRNA. Translation: BAE41852.1.
BC022737 mRNA. Translation: AAH22737.1. Different initiation.
CCDSiCCDS39021.1. [Q3TCV3-1]
RefSeqiNP_780646.2. NM_175437.3. [Q3TCV3-1]
UniGeneiMm.121705.

Genome annotation databases

EnsembliENSMUST00000036031; ENSMUSP00000043679; ENSMUSG00000039934. [Q3TCV3-1]
ENSMUST00000198071; ENSMUSP00000142407; ENSMUSG00000039934. [Q3TCV3-3]
GeneIDi212167.
KEGGimmu:212167.
UCSCiuc008woi.1. mouse. [Q3TCV3-2]
uc008woj.1. mouse. [Q3TCV3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033694 mRNA. Translation: BAC28433.1. Frameshift.
AK080244 mRNA. Translation: BAC37858.1.
AK137494 mRNA. Translation: BAE23378.1.
AK170515 mRNA. Translation: BAE41852.1.
BC022737 mRNA. Translation: AAH22737.1. Different initiation.
CCDSiCCDS39021.1. [Q3TCV3-1]
RefSeqiNP_780646.2. NM_175437.3. [Q3TCV3-1]
UniGeneiMm.121705.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59239N.
STRINGi10090.ENSMUSP00000043679.

PTM databases

iPTMnetiQ3TCV3.
PhosphoSiteiQ3TCV3.

Proteomic databases

MaxQBiQ3TCV3.
PaxDbiQ3TCV3.
PRIDEiQ3TCV3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036031; ENSMUSP00000043679; ENSMUSG00000039934. [Q3TCV3-1]
ENSMUST00000198071; ENSMUSP00000142407; ENSMUSG00000039934. [Q3TCV3-3]
GeneIDi212167.
KEGGimmu:212167.
UCSCiuc008woi.1. mouse. [Q3TCV3-2]
uc008woj.1. mouse. [Q3TCV3-1]

Organism-specific databases

CTDi54103.
MGIiMGI:2442259. Gsap.

Phylogenomic databases

eggNOGiENOG410IG5Q. Eukaryota.
ENOG41129DZ. LUCA.
GeneTreeiENSGT00390000012875.
HOVERGENiHBG095500.
InParanoidiQ3TCV3.
OMAiSATRTCW.
OrthoDBiEOG767391.
PhylomeDBiQ3TCV3.
TreeFamiTF323853.

Miscellaneous databases

NextBioi373490.
PROiQ3TCV3.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TCV3.
ExpressionAtlasiQ3TCV3. baseline and differential.
GenevisibleiQ3TCV3. MM.

Family and domain databases

InterProiIPR028010. GSAP_C_dom.
IPR026172. GSAP_fam.
[Graphical view]
PANTHERiPTHR13630. PTHR13630. 1 hit.
PfamiPF14959. GSAP-16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Aorta, Bone, Cecum and Vein.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Eye.
  3. "Gamma-secretase activating protein is a therapeutic target for Alzheimer's disease."
    He G., Luo W., Li P., Remmers C., Netzer W.J., Hendrick J., Bettayeb K., Flajolet M., Gorelick F., Wennogle L.P., Greengard P.
    Nature 467:95-98(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGSAP_MOUSE
AccessioniPrimary (citable) accession number: Q3TCV3
Secondary accession number(s): Q3UV93
, Q8BJR8, Q8BZR8, Q8R1Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 11, 2005
Last modified: January 20, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.