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Protein

Putative phospholipase B-like 2

Gene

Plbd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative phospholipase.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Protein family/group databases

MEROPSiC95.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative phospholipase B-like 2 (EC:3.1.1.-)
Alternative name(s):
66.3 kDa protein
76 kDa protein
Short name:
p76
LAMA-like protein 2
Lamina ancestor homolog 2
Phospholipase B domain-containing protein 2
Cleaved into the following 3 chains:
Gene namesi
Name:Plbd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919022. Plbd2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • lysosomal lumen Source: UniProtKB-SubCell
  • lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646Add
BLAST
Chaini47 – 594548Putative phospholipase B-like 2PRO_0000286111Add
BLAST
Chaini47 – 248202Putative phospholipase B-like 2 28 kDa formPRO_0000314076Add
BLAST
Chaini249 – 594346Putative phospholipase B-like 2 40 kDa formPRO_0000314077Add
BLAST
Chaini514 – 59481Putative phospholipase B-like 2 15 kDa formPRO_0000314078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi93 – 931N-linked (GlcNAc...)1 Publication
Glycosylationi115 – 1151N-linked (GlcNAc...)2 Publications
Disulfide bondi147 ↔ 1571 Publication
Glycosylationi236 – 2361N-linked (GlcNAc...)2 Publications
Glycosylationi441 – 4411N-linked (GlcNAc...)2 Publications
Disulfide bondi497 ↔ 5001 Publication
Glycosylationi520 – 5201N-linked (GlcNAc...)2 Publications

Post-translational modificationi

The p76 protein is synthesized as a 76 kDa precursor which is then processed into a N-terminal 28 kDa form and a C-terminal 40 kDa form. The C-terminal peptide is further processed into a 15 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ3TCN2.
MaxQBiQ3TCN2.
PaxDbiQ3TCN2.
PeptideAtlasiQ3TCN2.
PRIDEiQ3TCN2.

PTM databases

iPTMnetiQ3TCN2.
PhosphoSiteiQ3TCN2.

Expressioni

Tissue specificityi

Present at highest levels in spleen, lung and brain (at protein level).1 Publication

Gene expression databases

BgeeiQ3TCN2.
CleanExiMM_1300012G16RIK.
GenevisibleiQ3TCN2. MM.

Interactioni

Subunit structurei

Interacts with IGF2R.By similarity

Protein-protein interaction databases

IntActiQ3TCN2. 2 interactions.
MINTiMINT-4786130.
STRINGi10090.ENSMUSP00000031597.

Structurei

Secondary structure

1
594
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi64 – 729Combined sources
Turni73 – 764Combined sources
Beta strandi77 – 837Combined sources
Beta strandi88 – 969Combined sources
Helixi98 – 1014Combined sources
Beta strandi104 – 1107Combined sources
Helixi116 – 14227Combined sources
Turni143 – 1475Combined sources
Helixi154 – 17724Combined sources
Helixi182 – 20221Combined sources
Turni216 – 2194Combined sources
Helixi220 – 2234Combined sources
Helixi225 – 23410Combined sources
Beta strandi250 – 2556Combined sources
Helixi257 – 2593Combined sources
Beta strandi262 – 2676Combined sources
Beta strandi269 – 2713Combined sources
Helixi272 – 2743Combined sources
Beta strandi278 – 2836Combined sources
Beta strandi287 – 2915Combined sources
Beta strandi300 – 3067Combined sources
Beta strandi317 – 3204Combined sources
Beta strandi323 – 3308Combined sources
Helixi336 – 3416Combined sources
Beta strandi344 – 3463Combined sources
Helixi350 – 36011Combined sources
Helixi364 – 3718Combined sources
Turni372 – 3743Combined sources
Beta strandi381 – 3877Combined sources
Helixi388 – 3903Combined sources
Beta strandi399 – 40911Combined sources
Beta strandi412 – 4176Combined sources
Helixi419 – 4257Combined sources
Beta strandi426 – 4305Combined sources
Helixi437 – 4426Combined sources
Helixi445 – 4528Combined sources
Helixi454 – 4563Combined sources
Turni458 – 4603Combined sources
Helixi462 – 4709Combined sources
Helixi471 – 4733Combined sources
Helixi477 – 4848Combined sources
Turni489 – 4913Combined sources
Helixi493 – 4953Combined sources
Beta strandi500 – 5045Combined sources
Beta strandi508 – 5114Combined sources
Helixi514 – 5163Combined sources
Helixi526 – 5283Combined sources
Beta strandi532 – 5354Combined sources
Beta strandi537 – 5415Combined sources
Helixi543 – 5475Combined sources
Beta strandi551 – 5577Combined sources
Beta strandi560 – 5623Combined sources
Helixi567 – 5693Combined sources
Turni571 – 5744Combined sources
Beta strandi582 – 5843Combined sources
Beta strandi589 – 5913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FBXX-ray2.40A47-594[»]
3FGRX-ray1.80A47-248[»]
B249-594[»]
3FGTX-ray2.40A47-248[»]
B249-594[»]
3FGWX-ray2.80A47-594[»]
ProteinModelPortaliQ3TCN2.
SMRiQ3TCN2. Positions 60-594.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3TCN2.

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase B-like family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOVERGENiHBG096591.
InParanoidiQ3TCN2.
OMAiTNAIRET.
OrthoDBiEOG7D2FD5.
PhylomeDBiQ3TCN2.
TreeFamiTF315042.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TCN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVDGSSG GWAARALRRA LALTSLTTLA LLASLTGLLL SGPAGALPTL
60 70 80 90 100
GPGWQRQNPD PPVSRTRSLL LDAASGQLRL EDGFHPDAVA WANLTNAIRE
110 120 130 140 150
TGWAYLDLST NGRYNDSLQA YAAGVVEASV SEELIYMHWM NTVVNYCGPF
160 170 180 190 200
EYEVGYCEKL KNFLEANLEW MQREMELNPD SPYWHQVRLT LLQLKGLEDS
210 220 230 240 250
YEGRLTFPTG RFTIKPLGFL LLQISGDLED LEPALNKTNT KPSLGSGSCS
260 270 280 290 300
ALIKLLPGGH DLLVAHNTWN SYQNMLRIIK KYRLQFREGP QEEYPLVAGN
310 320 330 340 350
NLVFSSYPGT IFSGDDFYIL GSGLVTLETT IGNKNPALWK YVQPQGCVLE
360 370 380 390 400
WIRNVVANRL ALDGATWADV FKRFNSGTYN NQWMIVDYKA FLPNGPSPGS
410 420 430 440 450
RVLTILEQIP GMVVVADKTA ELYKTTYWAS YNIPYFETVF NASGLQALVA
460 470 480 490 500
QYGDWFSYTK NPRAKIFQRD QSLVEDMDAM VRLMRYNDFL HDPLSLCEAC
510 520 530 540 550
NPKPNAENAI SARSDLNPAN GSYPFQALHQ RAHGGIDVKV TSFTLAKYMS
560 570 580 590
MLAASGPTWD QCPPFQWSKS PFHSMLHMGQ PDLWMFSPIR VPWD
Length:594
Mass (Da):66,289
Last modified:May 1, 2007 - v2
Checksum:i202556A56C1AD0BA
GO
Isoform 2 (identifier: Q3TCN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-451: FETVFNASGLQALVAQ → VALFFHVAPSPQVCPP
     452-594: Missing.

Note: No experimental confirmation available.
Show »
Length:451
Mass (Da):49,936
Checksum:i8E6774FC602A9495
GO

Sequence cautioni

The sequence AAH26395.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → S in BAC28089 (PubMed:16141072).Curated
Sequence conflicti91 – 911W → L in BAC28089 (PubMed:16141072).Curated
Sequence conflicti107 – 1071D → G in BAE41761 (PubMed:16141072).Curated
Sequence conflicti173 – 1731R → M in BAB23709 (PubMed:16141072).Curated
Sequence conflicti223 – 2231Q → H in BAB23709 (PubMed:16141072).Curated
Sequence conflicti227 – 2271D → Y in BAB23709 (PubMed:16141072).Curated
Sequence conflicti230 – 2301D → N in BAB23709 (PubMed:16141072).Curated
Sequence conflicti232 – 2321E → K in BAB23709 (PubMed:16141072).Curated
Sequence conflicti315 – 3151D → N in BAB23709 (PubMed:16141072).Curated
Sequence conflicti348 – 3481V → E in BAE37687 (PubMed:16141072).Curated
Sequence conflicti381 – 3811N → S in AAG44101 (PubMed:12080145).Curated
Sequence conflicti553 – 5531A → T in BAE41924 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei436 – 45116FETVF…ALVAQ → VALFFHVAPSPQVCPP in isoform 2. 1 PublicationVSP_024998Add
BLAST
Alternative sequencei452 – 594143Missing in isoform 2. 1 PublicationVSP_024999Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217003 mRNA. Translation: AAG44101.1.
AK004973 mRNA. Translation: BAB23709.1.
AK030234 mRNA. Translation: BAC26858.1.
AK032930 mRNA. Translation: BAC28089.1.
AK042089 mRNA. Translation: BAC31160.1.
AK043608 mRNA. Translation: BAC31595.1.
AK046937 mRNA. Translation: BAC32923.1.
AK164220 mRNA. Translation: BAE37687.1.
AK170387 mRNA. Translation: BAE41761.1.
AK170631 mRNA. Translation: BAE41924.1.
BC026395 mRNA. Translation: AAH26395.1. Different initiation.
BC038605 mRNA. Translation: AAH38605.1.
BC048826 mRNA. Translation: AAH48826.1.
CCDSiCCDS19620.1. [Q3TCN2-1]
RefSeqiNP_076114.2. NM_023625.4. [Q3TCN2-1]
UniGeneiMm.100065.

Genome annotation databases

EnsembliENSMUST00000031597; ENSMUSP00000031597; ENSMUSG00000029598. [Q3TCN2-1]
GeneIDi71772.
KEGGimmu:71772.
UCSCiuc008zhh.2. mouse. [Q3TCN2-1]
uc008zhi.2. mouse. [Q3TCN2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217003 mRNA. Translation: AAG44101.1.
AK004973 mRNA. Translation: BAB23709.1.
AK030234 mRNA. Translation: BAC26858.1.
AK032930 mRNA. Translation: BAC28089.1.
AK042089 mRNA. Translation: BAC31160.1.
AK043608 mRNA. Translation: BAC31595.1.
AK046937 mRNA. Translation: BAC32923.1.
AK164220 mRNA. Translation: BAE37687.1.
AK170387 mRNA. Translation: BAE41761.1.
AK170631 mRNA. Translation: BAE41924.1.
BC026395 mRNA. Translation: AAH26395.1. Different initiation.
BC038605 mRNA. Translation: AAH38605.1.
BC048826 mRNA. Translation: AAH48826.1.
CCDSiCCDS19620.1. [Q3TCN2-1]
RefSeqiNP_076114.2. NM_023625.4. [Q3TCN2-1]
UniGeneiMm.100065.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FBXX-ray2.40A47-594[»]
3FGRX-ray1.80A47-248[»]
B249-594[»]
3FGTX-ray2.40A47-248[»]
B249-594[»]
3FGWX-ray2.80A47-594[»]
ProteinModelPortaliQ3TCN2.
SMRiQ3TCN2. Positions 60-594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TCN2. 2 interactions.
MINTiMINT-4786130.
STRINGi10090.ENSMUSP00000031597.

Protein family/group databases

MEROPSiC95.001.

PTM databases

iPTMnetiQ3TCN2.
PhosphoSiteiQ3TCN2.

Proteomic databases

EPDiQ3TCN2.
MaxQBiQ3TCN2.
PaxDbiQ3TCN2.
PeptideAtlasiQ3TCN2.
PRIDEiQ3TCN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031597; ENSMUSP00000031597; ENSMUSG00000029598. [Q3TCN2-1]
GeneIDi71772.
KEGGimmu:71772.
UCSCiuc008zhh.2. mouse. [Q3TCN2-1]
uc008zhi.2. mouse. [Q3TCN2-2]

Organism-specific databases

CTDi196463.
MGIiMGI:1919022. Plbd2.

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOVERGENiHBG096591.
InParanoidiQ3TCN2.
OMAiTNAIRET.
OrthoDBiEOG7D2FD5.
PhylomeDBiQ3TCN2.
TreeFamiTF315042.

Miscellaneous databases

ChiTaRSiPlbd2. mouse.
EvolutionaryTraceiQ3TCN2.
PROiQ3TCN2.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TCN2.
CleanExiMM_1300012G16RIK.
GenevisibleiQ3TCN2. MM.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C3H/RV.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Brain cortex, Cerebellum, Liver, Testis, Thymus and Wolffian duct.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver and Salivary gland.
  4. "Molecular characterization of the hypothetical 66.3-kDa protein in mouse: lysosomal targeting, glycosylation, processing and tissue distribution."
    Deuschl F., Kollmann K., von Figura K., Luebke T.
    FEBS Lett. 580:5747-5752(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-93; ASN-115; ASN-236; ASN-441 AND ASN-520, SUBCELLULAR LOCATION, PROCESSING, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Biochemical characterization and lysosomal localization of the mannose-6-phosphate protein p76 (hypothetical protein LOC196463)."
    Jensen A.G., Chemali M., Chapel A., Kieffer-Jaquinod S., Jadot M., Garin J., Journet A.
    Biochem. J. 402:449-458(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PROCESSING.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse."
    Lakomek K., Dickmanns A., Mueller U., Kollmann K., Deuschl F., Berndt A., Lubke T., Ficner R.
    Acta Crystallogr. D 65:220-228(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 47-594, GLYCOSYLATION AT ASN-115; ASN-236; ASN-441 AND ASN-520, DISULFIDE BONDS.

Entry informationi

Entry nameiPLBL2_MOUSE
AccessioniPrimary (citable) accession number: Q3TCN2
Secondary accession number(s): Q3TD43
, Q3TPP8, Q8BHG8, Q8BMB6, Q8BXI3, Q8C0M4, Q8R0V3, Q9DBG4, Q9EQI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: July 6, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.