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Protein

Putative phospholipase B-like 2

Gene

Plbd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative phospholipase.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Protein family/group databases

MEROPSiC95.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative phospholipase B-like 2 (EC:3.1.1.-)
Alternative name(s):
66.3 kDa protein
76 kDa protein
Short name:
p76
LAMA-like protein 2
Lamina ancestor homolog 2
Phospholipase B domain-containing protein 2
Cleaved into the following 3 chains:
Gene namesi
Name:Plbd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919022. Plbd2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • lysosomal lumen Source: UniProtKB-SubCell
  • lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46Add BLAST46
ChainiPRO_000028611147 – 594Putative phospholipase B-like 2Add BLAST548
ChainiPRO_000031407647 – 248Putative phospholipase B-like 2 28 kDa formAdd BLAST202
ChainiPRO_0000314077249 – 594Putative phospholipase B-like 2 40 kDa formAdd BLAST346
ChainiPRO_0000314078514 – 594Putative phospholipase B-like 2 15 kDa formAdd BLAST81

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...)1 Publication1
Glycosylationi115N-linked (GlcNAc...)2 Publications1
Disulfide bondi147 ↔ 1571 Publication
Glycosylationi236N-linked (GlcNAc...)2 Publications1
Glycosylationi441N-linked (GlcNAc...)2 Publications1
Disulfide bondi497 ↔ 5001 Publication
Glycosylationi520N-linked (GlcNAc...)2 Publications1

Post-translational modificationi

The p76 protein is synthesized as a 76 kDa precursor which is then processed into a N-terminal 28 kDa form and a C-terminal 40 kDa form. The C-terminal peptide is further processed into a 15 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ3TCN2.
MaxQBiQ3TCN2.
PaxDbiQ3TCN2.
PeptideAtlasiQ3TCN2.
PRIDEiQ3TCN2.

PTM databases

iPTMnetiQ3TCN2.
PhosphoSitePlusiQ3TCN2.

Expressioni

Tissue specificityi

Present at highest levels in spleen, lung and brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000029598.
CleanExiMM_1300012G16RIK.
GenevisibleiQ3TCN2. MM.

Interactioni

Subunit structurei

Interacts with IGF2R.By similarity

Protein-protein interaction databases

IntActiQ3TCN2. 2 interactors.
MINTiMINT-4786130.
STRINGi10090.ENSMUSP00000031597.

Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi64 – 72Combined sources9
Turni73 – 76Combined sources4
Beta strandi77 – 83Combined sources7
Beta strandi88 – 96Combined sources9
Helixi98 – 101Combined sources4
Beta strandi104 – 110Combined sources7
Helixi116 – 142Combined sources27
Turni143 – 147Combined sources5
Helixi154 – 177Combined sources24
Helixi182 – 202Combined sources21
Turni216 – 219Combined sources4
Helixi220 – 223Combined sources4
Helixi225 – 234Combined sources10
Beta strandi250 – 255Combined sources6
Helixi257 – 259Combined sources3
Beta strandi262 – 267Combined sources6
Beta strandi269 – 271Combined sources3
Helixi272 – 274Combined sources3
Beta strandi278 – 283Combined sources6
Beta strandi287 – 291Combined sources5
Beta strandi300 – 306Combined sources7
Beta strandi317 – 320Combined sources4
Beta strandi323 – 330Combined sources8
Helixi336 – 341Combined sources6
Beta strandi344 – 346Combined sources3
Helixi350 – 360Combined sources11
Helixi364 – 371Combined sources8
Turni372 – 374Combined sources3
Beta strandi381 – 387Combined sources7
Helixi388 – 390Combined sources3
Beta strandi399 – 409Combined sources11
Beta strandi412 – 417Combined sources6
Helixi419 – 425Combined sources7
Beta strandi426 – 430Combined sources5
Helixi437 – 442Combined sources6
Helixi445 – 452Combined sources8
Helixi454 – 456Combined sources3
Turni458 – 460Combined sources3
Helixi462 – 470Combined sources9
Helixi471 – 473Combined sources3
Helixi477 – 484Combined sources8
Turni489 – 491Combined sources3
Helixi493 – 495Combined sources3
Beta strandi500 – 504Combined sources5
Beta strandi508 – 511Combined sources4
Helixi514 – 516Combined sources3
Helixi526 – 528Combined sources3
Beta strandi532 – 535Combined sources4
Beta strandi537 – 541Combined sources5
Helixi543 – 547Combined sources5
Beta strandi551 – 557Combined sources7
Beta strandi560 – 562Combined sources3
Helixi567 – 569Combined sources3
Turni571 – 574Combined sources4
Beta strandi582 – 584Combined sources3
Beta strandi589 – 591Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FBXX-ray2.40A47-594[»]
3FGRX-ray1.80A47-248[»]
B249-594[»]
3FGTX-ray2.40A47-248[»]
B249-594[»]
3FGWX-ray2.80A47-594[»]
ProteinModelPortaliQ3TCN2.
SMRiQ3TCN2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3TCN2.

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase B-like family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOVERGENiHBG096591.
InParanoidiQ3TCN2.
OMAiKKYWFQF.
OrthoDBiEOG091G0G9H.
PhylomeDBiQ3TCN2.
TreeFamiTF315042.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TCN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVDGSSG GWAARALRRA LALTSLTTLA LLASLTGLLL SGPAGALPTL
60 70 80 90 100
GPGWQRQNPD PPVSRTRSLL LDAASGQLRL EDGFHPDAVA WANLTNAIRE
110 120 130 140 150
TGWAYLDLST NGRYNDSLQA YAAGVVEASV SEELIYMHWM NTVVNYCGPF
160 170 180 190 200
EYEVGYCEKL KNFLEANLEW MQREMELNPD SPYWHQVRLT LLQLKGLEDS
210 220 230 240 250
YEGRLTFPTG RFTIKPLGFL LLQISGDLED LEPALNKTNT KPSLGSGSCS
260 270 280 290 300
ALIKLLPGGH DLLVAHNTWN SYQNMLRIIK KYRLQFREGP QEEYPLVAGN
310 320 330 340 350
NLVFSSYPGT IFSGDDFYIL GSGLVTLETT IGNKNPALWK YVQPQGCVLE
360 370 380 390 400
WIRNVVANRL ALDGATWADV FKRFNSGTYN NQWMIVDYKA FLPNGPSPGS
410 420 430 440 450
RVLTILEQIP GMVVVADKTA ELYKTTYWAS YNIPYFETVF NASGLQALVA
460 470 480 490 500
QYGDWFSYTK NPRAKIFQRD QSLVEDMDAM VRLMRYNDFL HDPLSLCEAC
510 520 530 540 550
NPKPNAENAI SARSDLNPAN GSYPFQALHQ RAHGGIDVKV TSFTLAKYMS
560 570 580 590
MLAASGPTWD QCPPFQWSKS PFHSMLHMGQ PDLWMFSPIR VPWD
Length:594
Mass (Da):66,289
Last modified:May 1, 2007 - v2
Checksum:i202556A56C1AD0BA
GO
Isoform 2 (identifier: Q3TCN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-451: FETVFNASGLQALVAQ → VALFFHVAPSPQVCPP
     452-594: Missing.

Note: No experimental confirmation available.
Show »
Length:451
Mass (Da):49,936
Checksum:i8E6774FC602A9495
GO

Sequence cautioni

The sequence AAH26395 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → S in BAC28089 (PubMed:16141072).Curated1
Sequence conflicti91W → L in BAC28089 (PubMed:16141072).Curated1
Sequence conflicti107D → G in BAE41761 (PubMed:16141072).Curated1
Sequence conflicti173R → M in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti223Q → H in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti227D → Y in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti230D → N in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti232E → K in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti315D → N in BAB23709 (PubMed:16141072).Curated1
Sequence conflicti348V → E in BAE37687 (PubMed:16141072).Curated1
Sequence conflicti381N → S in AAG44101 (PubMed:12080145).Curated1
Sequence conflicti553A → T in BAE41924 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024998436 – 451FETVF…ALVAQ → VALFFHVAPSPQVCPP in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_024999452 – 594Missing in isoform 2. 1 PublicationAdd BLAST143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217003 mRNA. Translation: AAG44101.1.
AK004973 mRNA. Translation: BAB23709.1.
AK030234 mRNA. Translation: BAC26858.1.
AK032930 mRNA. Translation: BAC28089.1.
AK042089 mRNA. Translation: BAC31160.1.
AK043608 mRNA. Translation: BAC31595.1.
AK046937 mRNA. Translation: BAC32923.1.
AK164220 mRNA. Translation: BAE37687.1.
AK170387 mRNA. Translation: BAE41761.1.
AK170631 mRNA. Translation: BAE41924.1.
BC026395 mRNA. Translation: AAH26395.1. Different initiation.
BC038605 mRNA. Translation: AAH38605.1.
BC048826 mRNA. Translation: AAH48826.1.
CCDSiCCDS19620.1. [Q3TCN2-1]
RefSeqiNP_076114.2. NM_023625.4. [Q3TCN2-1]
UniGeneiMm.100065.

Genome annotation databases

EnsembliENSMUST00000031597; ENSMUSP00000031597; ENSMUSG00000029598. [Q3TCN2-1]
GeneIDi71772.
KEGGimmu:71772.
UCSCiuc008zhh.2. mouse. [Q3TCN2-1]
uc008zhi.2. mouse. [Q3TCN2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217003 mRNA. Translation: AAG44101.1.
AK004973 mRNA. Translation: BAB23709.1.
AK030234 mRNA. Translation: BAC26858.1.
AK032930 mRNA. Translation: BAC28089.1.
AK042089 mRNA. Translation: BAC31160.1.
AK043608 mRNA. Translation: BAC31595.1.
AK046937 mRNA. Translation: BAC32923.1.
AK164220 mRNA. Translation: BAE37687.1.
AK170387 mRNA. Translation: BAE41761.1.
AK170631 mRNA. Translation: BAE41924.1.
BC026395 mRNA. Translation: AAH26395.1. Different initiation.
BC038605 mRNA. Translation: AAH38605.1.
BC048826 mRNA. Translation: AAH48826.1.
CCDSiCCDS19620.1. [Q3TCN2-1]
RefSeqiNP_076114.2. NM_023625.4. [Q3TCN2-1]
UniGeneiMm.100065.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FBXX-ray2.40A47-594[»]
3FGRX-ray1.80A47-248[»]
B249-594[»]
3FGTX-ray2.40A47-248[»]
B249-594[»]
3FGWX-ray2.80A47-594[»]
ProteinModelPortaliQ3TCN2.
SMRiQ3TCN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TCN2. 2 interactors.
MINTiMINT-4786130.
STRINGi10090.ENSMUSP00000031597.

Protein family/group databases

MEROPSiC95.001.

PTM databases

iPTMnetiQ3TCN2.
PhosphoSitePlusiQ3TCN2.

Proteomic databases

EPDiQ3TCN2.
MaxQBiQ3TCN2.
PaxDbiQ3TCN2.
PeptideAtlasiQ3TCN2.
PRIDEiQ3TCN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031597; ENSMUSP00000031597; ENSMUSG00000029598. [Q3TCN2-1]
GeneIDi71772.
KEGGimmu:71772.
UCSCiuc008zhh.2. mouse. [Q3TCN2-1]
uc008zhi.2. mouse. [Q3TCN2-2]

Organism-specific databases

CTDi196463.
MGIiMGI:1919022. Plbd2.

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOVERGENiHBG096591.
InParanoidiQ3TCN2.
OMAiKKYWFQF.
OrthoDBiEOG091G0G9H.
PhylomeDBiQ3TCN2.
TreeFamiTF315042.

Miscellaneous databases

ChiTaRSiPlbd2. mouse.
EvolutionaryTraceiQ3TCN2.
PROiQ3TCN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029598.
CleanExiMM_1300012G16RIK.
GenevisibleiQ3TCN2. MM.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLBL2_MOUSE
AccessioniPrimary (citable) accession number: Q3TCN2
Secondary accession number(s): Q3TD43
, Q3TPP8, Q8BHG8, Q8BMB6, Q8BXI3, Q8C0M4, Q8R0V3, Q9DBG4, Q9EQI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.