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Protein

BRISC complex subunit Abraxas 2

Gene

Abraxas2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates. May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm (By similarity). Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins (PubMed:21195082). Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). Required for normal induction of p53/TP53 in response to DNA damage. Independent of the BRISC complex, promotes interaction between USP7 and p53/TP53, and thereby promotes deubiquitination of p53/TP53, preventing its degradation and resulting in increased p53/TP53-mediated transcription regulation and p53/TP53-dependent apoptosis in response to DNA damage (By similarity).By similarity1 Publication2 Publications

Miscellaneous

Interaction with THAP5 has been shown in human, but rodents lack a THAP5 homolog.Curated

GO - Molecular functioni

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • cellular response to freezing Source: MGI
  • chromosome segregation Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • mitotic spindle assembly Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • protein K63-linked deubiquitination Source: UniProtKB
  • response to ischemia Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
BRISC complex subunit Abraxas 2Imported
Alternative name(s):
Abraxas brother protein 1
Protein FAM175B
Gene namesi
Name:Abraxas2Imported
Synonyms:Abro11 Publication, Fam175b, Kiaa0157
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1926116. Abraxas2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, and are protected against toxic shock caused by bacterial lipopolysaccharide (LPS). IFNAR1 'Lys-63'-linked ubiquitination and IFNAR1 internalization are increased.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507261 – 415BRISC complex subunit Abraxas 2Add BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei274PhosphoserineBy similarity1
Modified residuei280PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei375PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3TCJ1.
MaxQBiQ3TCJ1.
PaxDbiQ3TCJ1.
PeptideAtlasiQ3TCJ1.
PRIDEiQ3TCJ1.

PTM databases

iPTMnetiQ3TCJ1.
PhosphoSitePlusiQ3TCJ1.

Expressioni

Tissue specificityi

Detected in heart (at protein level).1 Publication

Inductioni

Up-regulated in heart in response to ischemia and reperfusion (at protein level).1 Publication

Interactioni

Subunit structurei

Component of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with BRCC3/BRCC36; the interaction is direct. Interacts with BABAM1. Does not interact with BRCA1. Interacts with SHMT1 and SHMT2; the interaction is direct. Identified in a complex with SHMT2 and the other subunits of the BRISC complex. The BRISC complex binds monoubiquitin and both 'Lys-48'- and 'Lys-63'-linked polyubiquitin. Identified in complexes with IFNAR1, IFNAR2 and SHMT2. Interacts with THAP5 (By similarity). Interacts with ATF4 (PubMed:22974638). Identified in a complex with p53/TP53 and USP7; interacts directly with both proteins. Interacts with NUMA1. Interacts with microtubule minus ends. Binds polyubiquitin (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224665. 5 interactors.
STRINGi10090.ENSMUSP00000081541.

Structurei

3D structure databases

ProteinModelPortaliQ3TCJ1.
SMRiQ3TCJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 149MPNPROSITE-ProRule annotationAdd BLAST147

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 222Important for interaction with SHMT2By similarity8
Regioni220 – 241Important for interaction with BBRC36 and other subunits of the BRISC complexBy similarityAdd BLAST22

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili215 – 266Sequence analysisAdd BLAST52

Sequence similaritiesi

Belongs to the FAM175 family. Abro1 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IPEG. Eukaryota.
ENOG410ZUYJ. LUCA.
HOGENOMiHOG000112450.
HOVERGENiHBG081817.
InParanoidiQ3TCJ1.
KOiK20799.
PhylomeDBiQ3TCJ1.
TreeFamiTF331751.

Family and domain databases

InterProiView protein in InterPro
IPR023238. FAM175.
IPR023240. FAM175_BRISC_cplx_Abro1_su.
PRINTSiPR02053. BRISCABRO1.
PR02051. PROTEINF175.
PROSITEiView protein in PROSITE
PS50249. MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3TCJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASISGYTF SAVCFHSANS NADHEGFLLG EVRQEETFSI SDSQISNTEF
60 70 80 90 100
LQVIEIHNHQ PCSQLFSFYD YASKVNEESL DRILKDRRKK VIGWYRFRRN
110 120 130 140 150
TQQQMSYREQ VIHKQLTRIL GVPDLVFLLF SFISTANNST HALEYVLFRP
160 170 180 190 200
NRRYNQRISL AIPNLGNTSQ QEYKVSSVPN TSQSYAKVIK EHGTDFFDKD
210 220 230 240 250
GVMKDIRAIY QVYNALQEKV QAVCADVEKS ERVVESCQAE VNKLRRQITQ
260 270 280 290 300
KKNEKEQERR LQQALLSRQM PSESLEPAFS PRMSYSGFSA EGRSTLAETE
310 320 330 340 350
PSDPPPPYSD FHPNNQESTL SHSRMERSVF MPRPQAVGSS SYASTSGGLK
360 370 380 390 400
FTGSGADLLP SQSAAGDSGE ESDDSDYENL IDPAESPHSE YSHSKNSRPS
410
THPDEDPRNT QTSQI
Length:415
Mass (Da):46,943
Last modified:October 11, 2005 - v1
Checksum:iC9CB374DA089AFA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK170704 mRNA. Translation: BAE41965.1.
AK171524 mRNA. Translation: BAE42506.1.
BC030838 mRNA. Translation: AAH30838.2.
RefSeqiNP_932134.3. NM_198017.3.
UniGeneiMm.274824.
Mm.465624.

Genome annotation databases

GeneIDi109359.
KEGGimmu:109359.
UCSCiuc009kcr.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiABRX2_MOUSE
AccessioniPrimary (citable) accession number: Q3TCJ1
Secondary accession number(s): Q3TB08, Q8K0R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: October 25, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although strongly related to the ABRAXAS1 protein, lacks the C-terminal pSXXF that constitutes a specific recognition motif for the BRCT domain of BRCA1.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families