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Protein

Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

Alg6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT57 Glycosyltransferase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6 homolog
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:Alg6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444031 Alg6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Transmembranei227 – 247HelicalSequence analysisAdd BLAST21
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Transmembranei362 – 382HelicalSequence analysisAdd BLAST21
Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Transmembranei473 – 493HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002841321 – 507Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST507

Proteomic databases

MaxQBiQ3TAE8
PaxDbiQ3TAE8
PeptideAtlasiQ3TAE8
PRIDEiQ3TAE8

PTM databases

PhosphoSitePlusiQ3TAE8

Expressioni

Gene expression databases

BgeeiENSMUSG00000073792
ExpressionAtlasiQ3TAE8 baseline and differential
GenevisibleiQ3TAE8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095574

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2575 Eukaryota
ENOG410XQK1 LUCA
GeneTreeiENSGT00550000075073
HOGENOMiHOG000195048
HOVERGENiHBG024331
InParanoidiQ3TAE8
KOiK03848
OMAiFINPDWI
OrthoDBiEOG091G0AVE
PhylomeDBiQ3TAE8
TreeFamiTF314522

Family and domain databases

InterProiView protein in InterPro
IPR004856 Glyco_trans_ALG6/ALG8
PANTHERiPTHR12413 PTHR12413, 1 hit
PfamiView protein in Pfam
PF03155 Alg6_Alg8, 1 hit

Sequencei

Sequence statusi: Complete.

Q3TAE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESWPWMAVV VLLGLTVRWT VSLSSYSGAG KPPMFGDYEA QRHWQEITLN
60 70 80 90 100
LPVKQWYFNS SDNNLLYWGL DYPPLTAYHS LLCAYVAKFI NPDWVALHTS
110 120 130 140 150
RGYESQAHKL FMRATVLAAD LLIYVPAVLL YCYSLKEISP KRKIASALCI
160 170 180 190 200
LLYPGLILID YGHFQYNSVS LGFALWGVLG VSWDWDLLGS LAFCLALNYK
210 220 230 240 250
QMELYHSLPF FCFLLGKCFK KGLKGKGLAL FIRIACTVLA SFLLCWLPFL
260 270 280 290 300
TEREHALQVV RRLFPVDRGL FEDKVANIWC SVNVFLKIKD TLPRHIQIAI
310 320 330 340 350
SFCFTLLSLL PACIKLTVRP SCKGFRFTLV SCALSFFLFS FQVHEKSILL
360 370 380 390 400
VSLPVCLVLT EIPFMSTWFL LVSTFSMLPL LLKDELLLPS VVTVMAFVIA
410 420 430 440 450
CGTFFPMLEN TSEEQLQLKS FAVSVRRHLP GFTFLPRIMQ CLFLSSVITM
460 470 480 490 500
VLLTILSVTL DPPQKLPDLF PVLICFVSCV NFVFFLVYFN IVIMWDSKNG

RNRKKIE
Length:507
Mass (Da):57,874
Last modified:October 11, 2005 - v1
Checksum:i55D661178739A2CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442L → F in BAE34254 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK157903 mRNA Translation: BAE34254.1
AK171887 mRNA Translation: BAE42720.1
BX005053 Genomic DNA No translation available.
BC050854 mRNA Translation: AAH50854.1
CCDSiCCDS38818.1
RefSeqiNP_001074733.1, NM_001081264.1
UniGeneiMm.32408

Genome annotation databases

EnsembliENSMUST00000097961; ENSMUSP00000095574; ENSMUSG00000073792
ENSMUST00000228995; ENSMUSP00000155066; ENSMUSG00000116382
GeneIDi320438
KEGGimmu:320438
UCSCiuc008tuw.1 mouse

Similar proteinsi

Entry informationi

Entry nameiALG6_MOUSE
AccessioniPrimary (citable) accession number: Q3TAE8
Secondary accession number(s): Q3TZF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 11, 2005
Last modified: June 20, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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