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Protein

Carboxylesterase 5A

Gene

CES5A

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.By similarity

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei227 – 2271Charge relay systemBy similarity
Active sitei336 – 3361Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

ESTHERisheep-cauxin. Carb_B_Chordata.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxylesterase 5A (EC:3.1.1.1)
Alternative name(s):
Carboxylesterase-like urinary excreted protein homolog
Short name:
Cauxin
Gene namesi
Name:CES5A
Synonyms:CES7
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›381›381Carboxylesterase 5APRO_0000308594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi162 ↔ 173By similarity
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Detected in corpus and cauda epididymal fluid. Present in seminal fluid but not found to be associated with sperm (at protein level). Not expressed in other tissues.1 Publication

Interactioni

Subunit structurei

Component of a epididymal complex at least composed of soluble form of prion protein PRNP, CLU, BPI, CES5A, MANBA and GLB1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ3T930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG008839.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q3T930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
EDCLYLNIYA PAHAETGSKL PVMVWFPGGA FETGSASIFD GSALASYENV
60 70 80 90 100
LVVTIQYRLG IFGFFNTGDE HARGNWAFMD QVAALVWVQE NIEFFGGDPR
110 120 130 140 150
CVTIFGESAG AISVSSLILS PMTKGLFHKA IMASGVAIIP YLKASDYERN
160 170 180 190 200
DDLQTIASIC DCNASDSVAL LQCLRAKSSE ELLSISQKTK SFTRVVDGLF
210 220 230 240 250
FPNELLDLLA QKLFHLVPSI IGVNNHECGF LLPMKEFPEI LGGSNKSLAL
260 270 280 290 300
QLIHSVLHIP VQYSYLVADE YFHNKHSLLD IRNRFLDLLG DVFFVVPGLV
310 320 330 340 350
TAQYHTDAGA PVYFYEFQHR PQCLKDRKPP FVKADHTDEI RFVFGGAFLK
360 370 380
GNIVMFEEAT EEEKALSRKM MRYWANFART G
Length:381
Mass (Da):42,468
Last modified:October 11, 2005 - v1
Checksum:i38F19BDADD346EA4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei381 – 3811

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM075621 mRNA. Translation: CAJ27151.1.
UniGeneiOar.4790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM075621 mRNA. Translation: CAJ27151.1.
UniGeneiOar.4790.

3D structure databases

ProteinModelPortaliQ3T930.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERisheep-cauxin. Carb_B_Chordata.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008839.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The epididymal soluble prion protein forms a high-molecular-mass complex in association with hydrophobic proteins."
    Ecroyd H., Belghazi M., Dacheux J.-L., Gatti J.-L.
    Biochem. J. 392:211-219(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN COMPLEX WITH PRNP; CLU; BPI; MANBA AND GLB1.
    Tissue: Epididymis.
  2. "An epididymal form of cauxin, a carboxylesterase-like enzyme, is present and active in mammalian male reproductive fluids."
    Ecroyd H., Belghazi M., Dacheux J.-L., Miyazaki M., Yamashita T., Gatti J.-L.
    Biol. Reprod. 74:439-447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, GLYCOSYLATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiEST5A_SHEEP
AccessioniPrimary (citable) accession number: Q3T930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 11, 2005
Last modified: June 24, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.