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Q3T920 (Q3T920_THECA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL CAJ29881.1
OrganismThermus caldophilus EMBL CAJ29881.1
Taxonomic identifier272 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain120 – 315196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding147 – 19852ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2821Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2821Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2841Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q3T920 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 85E6F0AA367A13AF

FASTA31934,666
        10         20         30         40         50         60 
MRVLLIAGGV SPEHEVSLLS AEGVLRHIPF PTDLAVIAQD GRWLLGEKAL TALEAKAAPE 

        70         80         90        100        110        120 
GEHPFPPPLS WERYDVVFPL LHGRFGEDGT VQGFLELLGK PYVGAGVAAS ALCMDKDLSK 

       130        140        150        160        170        180 
RVLAQAGVPV VPWVAVRKGE PPVVPFDPPF FVKPANTGSS VGISRVERFQ DLEAALALAF 

       190        200        210        220        230        240 
RYDEKAVVEK ALSPVRELEV GVLGNVFGEA SPVGEVRYEA PFYDYETKYT PGRAELLIPA 

       250        260        270        280        290        300 
PLDPGTQETV QELALKAYKV LGVRGMARVD FFLAEGELYL NELNTIPGFT PTSMYPRLFE 

       310 
AGGVAYPELL RRLVELALT 

« Hide

References

[1]"Crystal Structure of Thermus caldophilus D-alanine:D-alanine ligase in the Open Conformation."
Lee J., Eom S., Lee D.S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Crystal structure of the apo form of D-alanine: D-alanine ligase (Ddl) from Thermus caldophilus: a basis for the substrate-induced conformational changes."
Lee J.H., Na Y., Song H.E., Kim D., Park B.H., Rho S.H., Im Y.J., Kim M.K., Kang G.B., Lee D.S., Eom S.H.
Proteins 64:1078-1082(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM084747 Genomic DNA. Translation: CAJ29881.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FB9X-ray1.90A1-319[»]
ProteinModelPortalQ3T920.
SMRQ3T920. Positions 1-319.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ3T920.

Entry information

Entry nameQ3T920_THECA
AccessionPrimary (citable) accession number: Q3T920
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)