Reviewed, UniProtKB/Swiss-Prot Q3T1L5 (ALG6_RAT)

Names and origin

Protein names

Recommended name:
    Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 6

Gene names

Name:

Alg6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000284133
Regions
	Transmembrane	4 – 24	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	144 – 164	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	298 – 318	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	362 – 382	21	Potential
	Transmembrane	388 – 408	21	Potential
	Transmembrane	438 – 458	21	Potential
	Transmembrane	473 – 493	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3T1L5-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: AFA556F410678B72	FASTA	507	57,948
	10 20 30 40 50 60 MESWTWMTVV VLLGLTVRWT VSLNSYSGAG KPPMFGDYEA QRHWQEITLN LPVKQWYFNS 70 80 90 100 110 120 SDNNLQYWGL DYPPLTAYHS LLCAYVAKFI NPDWVALHTS RGYESQAHKL FMRTTVLAAD 130 140 150 160 170 180 LLIYIPAVLL YCYSLKEISP KRKIASALCI LLYPGLILID YGHFQYNSVS LGFALWGVLG 190 200 210 220 230 240 VSCDWDLLGS LAFCLALNYK QMELYHSLPF FCFLLGKCFK KGLRGKGSAL FIRIACTVVA 250 260 270 280 290 300 SFLLCWLPFL TEREHALQVV RRLFPVDRGL FEDKVANIWC SLNVFLKIKD ILPRHIQIAI 310 320 330 340 350 360 SFCFTFLSLL PACIKLTVQP SAKGFRFTLV SCALSFFLFS FQVHEKSILL VSLPVCLVLT 370 380 390 400 410 420 EIPFMSTWFL LVSTFSMLPL LLKDQLLLPS VVTVMAFLIA CSTFFPMFEN TSEEQLQLKS 430 440 450 460 470 480 FAVSVRRHLP GFTFLPRIIQ CLFLSSVITM ILLTILSVTL DPPQKLPDLF SVLICFVSCV 490 500 NFVFFLVYFN IVIMWDSKNG RNRKKID

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC101850 mRNA. Translation: AAI01851.1.
IPI	IPI00358784.
RefSeq	NP_001028881.1.
UniGene	Rn.104225
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3T1L5.
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Genome annotation databases
Ensembl	ENSRNOT00000012052; ENSRNOP00000012052; ENSRNOG00000009045; Rattus norvegicus. [Genome view] ENSRNOT00000057874; ENSRNOP00000054683; ENSRNOG00000009045; Rattus norvegicus. [Genome view]
GeneID	362547.
KEGG	rno:362547.
Organism-specific databases
CTD	362547.
RGD	1308815. Alg6.
Phylogenomic databases
HOVERGEN	Q3T1L5.
Gene expression databases
Genevestigator	Q3T1L5.
Family and domain databases
InterPro	IPR004856. Glycosyltransferase_ALG6/ALG8. [Graphical view]
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	680317.

Entry information

Entry name

ALG6_RAT

Accession

Primary (citable) accession number: Q3T1L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 11, 2005
Last modified:	October 13, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)