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Protein

Eukaryotic translation initiation factor 5A-1

Gene

Eif5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival (By similarity). May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to thyroid hormone stimulus Source: RGD
  • mRNA transport Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cardiac muscle cell apoptotic process Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of muscle cell differentiation Source: RGD
  • positive regulation of reactive oxygen species metabolic process Source: RGD
  • positive regulation of translational elongation Source: InterPro
  • positive regulation of translational termination Source: InterPro
  • protein transport Source: UniProtKB-KW
  • translational frameshifting Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

mRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
eIF-4D
Gene namesi
Name:Eif5a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1308029. Eif5a.

Subcellular locationi

GO - Cellular componenti

  • dendrite Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 154153Eukaryotic translation initiation factor 5A-1PRO_0000229763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei47 – 471N6-acetyllysineBy similarity
Modified residuei50 – 501HypusineBy similarity
Modified residuei121 – 1211N6-acetyllysineBy similarity

Post-translational modificationi

Acetylated. Deacetylated by SIRT2 (By similarity).By similarity
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

PaxDbiQ3T1J1.
PRIDEiQ3T1J1.

PTM databases

iPTMnetiQ3T1J1.
PhosphoSiteiQ3T1J1.

Expressioni

Gene expression databases

GenevisibleiQ3T1J1. RN.

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi252165. 2 interactions.
IntActiQ3T1J1. 1 interaction.
MINTiMINT-4581054.
STRINGi10116.ENSRNOP00000022343.

Structurei

3D structure databases

ProteinModelPortaliQ3T1J1.
SMRiQ3T1J1. Positions 15-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
HOVERGENiHBG001104.
InParanoidiQ3T1J1.
KOiK03263.
OMAiMNEEAAI.
OrthoDBiEOG7KSXB7.
PhylomeDBiQ3T1J1.
TreeFamiTF101534.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3T1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v3
Checksum:i07EF043C7DEA3091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101891 mRNA. Translation: AAI01892.1.
RefSeqiNP_001028853.1. NM_001033681.1.
XP_006246697.1. XM_006246635.2.
XP_006246699.1. XM_006246637.2.
XP_006246700.1. XM_006246638.1.
UniGeneiRn.128212.

Genome annotation databases

EnsembliENSRNOT00000022343; ENSRNOP00000022343; ENSRNOG00000016478.
GeneIDi287444.
KEGGirno:287444.
UCSCiRGD:1308029. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101891 mRNA. Translation: AAI01892.1.
RefSeqiNP_001028853.1. NM_001033681.1.
XP_006246697.1. XM_006246635.2.
XP_006246699.1. XM_006246637.2.
XP_006246700.1. XM_006246638.1.
UniGeneiRn.128212.

3D structure databases

ProteinModelPortaliQ3T1J1.
SMRiQ3T1J1. Positions 15-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252165. 2 interactions.
IntActiQ3T1J1. 1 interaction.
MINTiMINT-4581054.
STRINGi10116.ENSRNOP00000022343.

PTM databases

iPTMnetiQ3T1J1.
PhosphoSiteiQ3T1J1.

Proteomic databases

PaxDbiQ3T1J1.
PRIDEiQ3T1J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022343; ENSRNOP00000022343; ENSRNOG00000016478.
GeneIDi287444.
KEGGirno:287444.
UCSCiRGD:1308029. rat.

Organism-specific databases

CTDi1984.
RGDi1308029. Eif5a.

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
HOVERGENiHBG001104.
InParanoidiQ3T1J1.
KOiK03263.
OMAiMNEEAAI.
OrthoDBiEOG7KSXB7.
PhylomeDBiQ3T1J1.
TreeFamiTF101534.

Enzyme and pathway databases

ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ3T1J1.

Gene expression databases

GenevisibleiQ3T1J1. RN.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 56-67 AND 87-109, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  3. "Insights on eukaryotic translation initiation factor 5A (eIF5A) in the brain and aging."
    Luchessi A.D., Cambiaghi T.D., Alves A.S., Parreiras-E-Silva L.T., Britto L.R.G., Costa-Neto C.M., Curi R.
    Brain Res. 1228:6-13(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Involvement of eukaryotic translation initiation factor 5A (eIF5A) in skeletal muscle stem cell differentiation."
    Luchessi A.D., Cambiaghi T.D., Hirabara S.M., Lambertucci R.H., Silveira L.R., Baptista I.L., Moriscot A.S., Costa-Neto C.M., Curi R.
    J. Cell. Physiol. 218:480-489(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIF5A1_RAT
AccessioniPrimary (citable) accession number: Q3T1J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.