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Protein

Eukaryotic translation initiation factor 5A-1

Gene

Eif5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival (By similarity). May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to thyroid hormone stimulus Source: RGD
  • mRNA transport Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cardiac muscle cell apoptotic process Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of muscle cell differentiation Source: RGD
  • positive regulation of reactive oxygen species metabolic process Source: RGD
  • positive regulation of translational elongation Source: InterPro
  • positive regulation of translational termination Source: InterPro
  • protein transport Source: UniProtKB-KW
  • translational frameshifting Source: InterPro

Keywordsi

Molecular functionElongation factor, RNA-binding
Biological processmRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-204626 Hypusine synthesis from eIF5A-lysine

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
eIF-4D
Gene namesi
Name:Eif5a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1308029 Eif5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002297632 – 154Eukaryotic translation initiation factor 5A-1Add BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei47N6-acetyllysineBy similarity1
Modified residuei50HypusineBy similarity1
Modified residuei121N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated. Deacetylated by SIRT2 (By similarity).By similarity
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

PaxDbiQ3T1J1
PRIDEiQ3T1J1

PTM databases

iPTMnetiQ3T1J1
PhosphoSitePlusiQ3T1J1
SwissPalmiQ3T1J1

Expressioni

Gene expression databases

BgeeiENSRNOG00000016478
GenevisibleiQ3T1J1 RN

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi252165, 2 interactors
IntActiQ3T1J1, 1 interactor
STRINGi10116.ENSRNOP00000022343

Structurei

3D structure databases

ProteinModelPortaliQ3T1J1
SMRiQ3T1J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271 Eukaryota
COG0231 LUCA
GeneTreeiENSGT00390000003738
HOGENOMiHOG000106270
HOVERGENiHBG001104
InParanoidiQ3T1J1
KOiK03263
OMAiCVIAIKT
OrthoDBiEOG091G0P2P
PhylomeDBiQ3T1J1
TreeFamiTF101534

Family and domain databases

Gene3Di2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR001884 IF5A-like
IPR012340 NA-bd_OB-fold
IPR014722 Rib_L2_dom2
IPR019769 Trans_elong_IF5A_hypusine_site
IPR020189 Transl_elong_IF5A_C
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11673 PTHR11673, 1 hit
PfamiView protein in Pfam
PF01287 eIF-5a, 1 hit
PIRSFiPIRSF003025 eIF5A, 1 hit
SMARTiView protein in SMART
SM01376 eIF-5a, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00037 eIF_5A, 1 hit
PROSITEiView protein in PROSITE
PS00302 IF5A_HYPUSINE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3T1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v3
Checksum:i07EF043C7DEA3091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101891 mRNA Translation: AAI01892.1
RefSeqiNP_001028853.1, NM_001033681.1
XP_006246699.1, XM_006246637.3
XP_006246700.1, XM_006246638.1
UniGeneiRn.128212

Genome annotation databases

EnsembliENSRNOT00000022343; ENSRNOP00000022343; ENSRNOG00000016478
GeneIDi287444
KEGGirno:287444
UCSCiRGD:1308029 rat

Similar proteinsi

Entry informationi

Entry nameiIF5A1_RAT
AccessioniPrimary (citable) accession number: Q3T1J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 111 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health