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Q3T145

- MDHC_BOVIN

UniProt

Q3T145 - MDHC_BOVIN

Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei98 – 981SubstrateBy similarity
    Binding sitei105 – 1051NADBy similarity
    Binding sitei112 – 1121NADBy similarity
    Binding sitei131 – 1311SubstrateBy similarity
    Binding sitei162 – 1621SubstrateBy similarity
    Active sitei187 – 1871Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 177NADBy similarity
    Nucleotide bindingi129 – 1313NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_227222. Gluconeogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
    Alternative name(s):
    Cytosolic malate dehydrogenase
    Gene namesi
    Name:MDH1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 334333Malate dehydrogenase, cytoplasmicPRO_0000226735Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei110 – 1101N6-succinyllysineBy similarity
    Modified residuei118 – 1181N6-acetyllysineBy similarity
    Modified residuei121 – 1211N6-acetyllysineBy similarity
    Modified residuei214 – 2141N6-succinyllysineBy similarity
    Modified residuei298 – 2981N6-acetyllysine; alternateBy similarity
    Modified residuei298 – 2981N6-succinyllysine; alternateBy similarity
    Modified residuei318 – 3181N6-succinyllysineBy similarity
    Modified residuei333 – 3331PhosphoserineBy similarity

    Post-translational modificationi

    ISGylated.By similarity
    Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ3T145.
    PRIDEiQ3T145.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025691.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3T145.
    SMRiQ3T145. Positions 2-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    GeneTreeiENSGT00530000063410.
    HOGENOMiHOG000220953.
    HOVERGENiHBG006340.
    InParanoidiQ3T145.
    KOiK00025.
    OMAiNCLIASK.
    OrthoDBiEOG78H3TM.
    TreeFamiTF105826.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3T145-1 [UniParc]FASTAAdd to Basket

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    MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL    50
    DGVLMELQDC ALPLLKDVIA TDKEEIAFKD LDVAILVGSM PRRDGMERKD 100
    LLKANVKIFK CQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE 150
    NFSCLTRLDH NRAKAQIALK LGVTSDDVKN VIIWGNHSST QYPDVNHAKV 200
    KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI 250
    CDHVRDIWFG TPEGEFVSMG IISDGNSYGI PDDLLYSFPV TIKDKTWKVV 300
    EGLPINDFSR EKMDLTAKEL AEEKETAFEF LASA 334
    Length:334
    Mass (Da):36,438
    Last modified:January 23, 2007 - v3
    Checksum:i72FA4DA47F46AB95
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC102133 mRNA. Translation: AAI02134.1.
    RefSeqiNP_001029800.1. NM_001034628.2.
    UniGeneiBt.5345.

    Genome annotation databases

    EnsembliENSBTAT00000025691; ENSBTAP00000025691; ENSBTAG00000019295.
    GeneIDi535182.
    KEGGibta:535182.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC102133 mRNA. Translation: AAI02134.1 .
    RefSeqi NP_001029800.1. NM_001034628.2.
    UniGenei Bt.5345.

    3D structure databases

    ProteinModelPortali Q3T145.
    SMRi Q3T145. Positions 2-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000025691.

    Proteomic databases

    PaxDbi Q3T145.
    PRIDEi Q3T145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000025691 ; ENSBTAP00000025691 ; ENSBTAG00000019295 .
    GeneIDi 535182.
    KEGGi bta:535182.

    Organism-specific databases

    CTDi 4190.

    Phylogenomic databases

    eggNOGi COG0039.
    GeneTreei ENSGT00530000063410.
    HOGENOMi HOG000220953.
    HOVERGENi HBG006340.
    InParanoidi Q3T145.
    KOi K00025.
    OMAi NCLIASK.
    OrthoDBi EOG78H3TM.
    TreeFami TF105826.

    Enzyme and pathway databases

    Reactomei REACT_227222. Gluconeogenesis.

    Miscellaneous databases

    NextBioi 20876650.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.

    Entry informationi

    Entry nameiMDHC_BOVIN
    AccessioniPrimary (citable) accession number: Q3T145
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 75 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3