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Q3T145

- MDHC_BOVIN

UniProt

Q3T145 - MDHC_BOVIN

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Protein
Malate dehydrogenase, cytoplasmic
Gene
MDH1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921Substrate By similarity
Binding sitei98 – 981Substrate By similarity
Binding sitei105 – 1051NAD By similarity
Binding sitei112 – 1121NAD By similarity
Binding sitei131 – 1311Substrate By similarity
Binding sitei162 – 1621Substrate By similarity
Active sitei187 – 1871Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NAD By similarity
Nucleotide bindingi129 – 1313NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_227222. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:MDH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 334333Malate dehydrogenase, cytoplasmicUniRule annotation
PRO_0000226735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei110 – 1101N6-succinyllysine By similarity
Modified residuei118 – 1181N6-acetyllysine By similarity
Modified residuei121 – 1211N6-acetyllysine By similarity
Modified residuei214 – 2141N6-succinyllysine By similarity
Modified residuei298 – 2981N6-acetyllysine; alternate By similarity
Modified residuei298 – 2981N6-succinyllysine; alternate By similarity
Modified residuei318 – 3181N6-succinyllysine By similarity
Modified residuei333 – 3331Phosphoserine By similarity

Post-translational modificationi

ISGylated By similarity.UniRule annotation
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3T145.
PRIDEiQ3T145.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025691.

Structurei

3D structure databases

ProteinModelPortaliQ3T145.
SMRiQ3T145. Positions 2-334.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiQ3T145.
KOiK00025.
OMAiNCLIASK.
OrthoDBiEOG78H3TM.
TreeFamiTF105826.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3T145-1 [UniParc]FASTAAdd to Basket

« Hide

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL    50
DGVLMELQDC ALPLLKDVIA TDKEEIAFKD LDVAILVGSM PRRDGMERKD 100
LLKANVKIFK CQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE 150
NFSCLTRLDH NRAKAQIALK LGVTSDDVKN VIIWGNHSST QYPDVNHAKV 200
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI 250
CDHVRDIWFG TPEGEFVSMG IISDGNSYGI PDDLLYSFPV TIKDKTWKVV 300
EGLPINDFSR EKMDLTAKEL AEEKETAFEF LASA 334
Length:334
Mass (Da):36,438
Last modified:January 23, 2007 - v3
Checksum:i72FA4DA47F46AB95
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102133 mRNA. Translation: AAI02134.1.
RefSeqiNP_001029800.1. NM_001034628.2.
UniGeneiBt.5345.

Genome annotation databases

EnsembliENSBTAT00000025691; ENSBTAP00000025691; ENSBTAG00000019295.
GeneIDi535182.
KEGGibta:535182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102133 mRNA. Translation: AAI02134.1 .
RefSeqi NP_001029800.1. NM_001034628.2.
UniGenei Bt.5345.

3D structure databases

ProteinModelPortali Q3T145.
SMRi Q3T145. Positions 2-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000025691.

Proteomic databases

PaxDbi Q3T145.
PRIDEi Q3T145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000025691 ; ENSBTAP00000025691 ; ENSBTAG00000019295 .
GeneIDi 535182.
KEGGi bta:535182.

Organism-specific databases

CTDi 4190.

Phylogenomic databases

eggNOGi COG0039.
GeneTreei ENSGT00530000063410.
HOGENOMi HOG000220953.
HOVERGENi HBG006340.
InParanoidi Q3T145.
KOi K00025.
OMAi NCLIASK.
OrthoDBi EOG78H3TM.
TreeFami TF105826.

Enzyme and pathway databases

Reactomei REACT_227222. Gluconeogenesis.

Miscellaneous databases

NextBioi 20876650.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiMDHC_BOVIN
AccessioniPrimary (citable) accession number: Q3T145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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