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Q3T145

- MDHC_BOVIN

UniProt

Q3T145 - MDHC_BOVIN

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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei105 – 1051NADBy similarity
Binding sitei112 – 1121NADBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei162 – 1621SubstrateBy similarity
Active sitei187 – 1871Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NADBy similarity
Nucleotide bindingi129 – 1313NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_227222. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:MDH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. extracellular space Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 334333Malate dehydrogenase, cytoplasmicPRO_0000226735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei110 – 1101N6-succinyllysineBy similarity
Modified residuei118 – 1181N6-acetyllysineBy similarity
Modified residuei121 – 1211N6-acetyllysineBy similarity
Modified residuei214 – 2141N6-succinyllysineBy similarity
Modified residuei298 – 2981N6-acetyllysine; alternateBy similarity
Modified residuei298 – 2981N6-succinyllysine; alternateBy similarity
Modified residuei318 – 3181N6-succinyllysineBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity

Post-translational modificationi

ISGylated.By similarity
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3T145.
PRIDEiQ3T145.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025691.

Structurei

3D structure databases

ProteinModelPortaliQ3T145.
SMRiQ3T145. Positions 2-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiQ3T145.
KOiK00025.
OMAiNCLIASK.
OrthoDBiEOG78H3TM.
TreeFamiTF105826.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3T145-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLKDVIA TDKEEIAFKD LDVAILVGSM PRRDGMERKD
110 120 130 140 150
LLKANVKIFK CQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKAQIALK LGVTSDDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
CDHVRDIWFG TPEGEFVSMG IISDGNSYGI PDDLLYSFPV TIKDKTWKVV
310 320 330
EGLPINDFSR EKMDLTAKEL AEEKETAFEF LASA
Length:334
Mass (Da):36,438
Last modified:January 23, 2007 - v3
Checksum:i72FA4DA47F46AB95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102133 mRNA. Translation: AAI02134.1.
RefSeqiNP_001029800.1. NM_001034628.2.
UniGeneiBt.5345.

Genome annotation databases

EnsembliENSBTAT00000025691; ENSBTAP00000025691; ENSBTAG00000019295.
GeneIDi535182.
KEGGibta:535182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102133 mRNA. Translation: AAI02134.1 .
RefSeqi NP_001029800.1. NM_001034628.2.
UniGenei Bt.5345.

3D structure databases

ProteinModelPortali Q3T145.
SMRi Q3T145. Positions 2-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000025691.

Proteomic databases

PaxDbi Q3T145.
PRIDEi Q3T145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000025691 ; ENSBTAP00000025691 ; ENSBTAG00000019295 .
GeneIDi 535182.
KEGGi bta:535182.

Organism-specific databases

CTDi 4190.

Phylogenomic databases

eggNOGi COG0039.
GeneTreei ENSGT00530000063410.
HOGENOMi HOG000220953.
HOVERGENi HBG006340.
InParanoidi Q3T145.
KOi K00025.
OMAi NCLIASK.
OrthoDBi EOG78H3TM.
TreeFami TF105826.

Enzyme and pathway databases

Reactomei REACT_227222. Gluconeogenesis.

Miscellaneous databases

NextBioi 20876650.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiMDHC_BOVIN
AccessioniPrimary (citable) accession number: Q3T145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3