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Protein

Peptidyl-tRNA hydrolase ICT1, mitochondrial

Gene

ICT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes (By similarity).By similarity

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-5389840. Mitochondrial translation elongation.
R-BTA-5419276. Mitochondrial translation termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase ICT1, mitochondrial (EC:3.1.1.29)
Alternative name(s):
39S ribosomal protein L58, mitochondrial
Short name:
MRP-L58
Immature colon carcinoma transcript 1 protein homolog
Gene namesi
Name:ICT1
Synonyms:MRPL58
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionSequence analysisAdd
BLAST
Chaini30 – 206177Peptidyl-tRNA hydrolase ICT1, mitochondrialPRO_0000045759Add
BLAST

Proteomic databases

PaxDbiQ3T116.
PRIDEiQ3T116.

Interactioni

Subunit structurei

Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004276.

Structurei

3D structure databases

ProteinModelPortaliQ3T116.
SMRiQ3T116. Positions 65-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3429. Eukaryota.
COG1186. LUCA.
GeneTreeiENSGT00390000013268.
HOGENOMiHOG000231063.
HOVERGENiHBG006115.
InParanoidiQ3T116.
KOiK15033.
OMAiTRSQQMN.
OrthoDBiEOG72G18Z.
TreeFamiTF315161.

Family and domain databases

InterProiIPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF00472. RF-1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3T116-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAARCLRWG LSRAEAWLLP PPTSCCHRAL HRQVEGTEFR SAYSLDKLYP
60 70 80 90 100
ESRGADTAWR VPGDAKQGND DIPVDRLTIS YCRSSGPGGQ NVNKVNSKAE
110 120 130 140 150
VRFHLASADW IAEPVRQKMA LTHKNKINRA GELILTSEYS RYQFRNLADC
160 170 180 190 200
LQKIRDMIAE ASQPATEPSK EDAALQKLRI ENMNRERLRK KRINSAIKTS

RRVGTD
Length:206
Mass (Da):23,307
Last modified:October 11, 2005 - v1
Checksum:i1369EF2674665516
GO
Isoform 2 (identifier: Q3T116-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-62: Missing.

Show »
Length:177
Mass (Da):20,023
Checksum:i8EC1619D1AEBE398
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei34 – 6229Missing in isoform 2. 1 PublicationVSP_039217Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102162 mRNA. Translation: AAI02163.1.
BC134736 mRNA. Translation: AAI34737.1.
RefSeqiNP_001029665.1. NM_001034493.1. [Q3T116-1]
XP_005221275.1. XM_005221218.3. [Q3T116-2]
UniGeneiBt.49175.

Genome annotation databases

EnsembliENSBTAT00000004276; ENSBTAP00000004276; ENSBTAG00000003303. [Q3T116-1]
GeneIDi515465.
KEGGibta:515465.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102162 mRNA. Translation: AAI02163.1.
BC134736 mRNA. Translation: AAI34737.1.
RefSeqiNP_001029665.1. NM_001034493.1. [Q3T116-1]
XP_005221275.1. XM_005221218.3. [Q3T116-2]
UniGeneiBt.49175.

3D structure databases

ProteinModelPortaliQ3T116.
SMRiQ3T116. Positions 65-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004276.

Proteomic databases

PaxDbiQ3T116.
PRIDEiQ3T116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000004276; ENSBTAP00000004276; ENSBTAG00000003303. [Q3T116-1]
GeneIDi515465.
KEGGibta:515465.

Organism-specific databases

CTDi3396.

Phylogenomic databases

eggNOGiKOG3429. Eukaryota.
COG1186. LUCA.
GeneTreeiENSGT00390000013268.
HOGENOMiHOG000231063.
HOVERGENiHBG006115.
InParanoidiQ3T116.
KOiK15033.
OMAiTRSQQMN.
OrthoDBiEOG72G18Z.
TreeFamiTF315161.

Enzyme and pathway databases

ReactomeiR-BTA-5389840. Mitochondrial translation elongation.
R-BTA-5419276. Mitochondrial translation termination.

Miscellaneous databases

NextBioi20871835.

Family and domain databases

InterProiIPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF00472. RF-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: Crossbred X Angus and Hereford.
    Tissue: Fetal brain and Ileum.

Entry informationi

Entry nameiICT1_BOVIN
AccessioniPrimary (citable) accession number: Q3T116
Secondary accession number(s): A7YWR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: October 11, 2005
Last modified: April 13, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site and determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.