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Q3T112

- PSB8_BOVIN

UniProt

Q3T112 - PSB8_BOVIN

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Protein

Proteasome subunit beta type-8

Gene

PSMB8

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides (By similarity). Required for adipocyte differentiation (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei72 – 732Cleavage; by autocatalysisBy similarity
Active sitei73 – 731NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. antigen processing and presentation Source: Ensembl
  2. fat cell differentiation Source: UniProtKB
  3. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Differentiation, Immunity

Enzyme and pathway databases

ReactomeiREACT_203739. Degradation of beta-catenin by the destruction complex.
REACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
REACT_205897. Activation of NF-kappaB in B cells.
REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
REACT_207857. Asymmetric localization of PCP proteins.
REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_208889. degradation of AXIN.
REACT_211738. ER-Phagosome pathway.
REACT_212887. Separation of Sister Chromatids.
REACT_213030. Orc1 removal from chromatin.
REACT_215163. degradation of DVL.
REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_237530. Regulation of ornithine decarboxylase (ODC).
REACT_252463. Hedgehog ligand biogenesis.
REACT_259250. Hh ligand biogenesis disease.
REACT_269173. Degradation of GLI2 by the proteasome.
REACT_269531. GLI3 is processed to GLI3R by the proteasome.
REACT_271133. Degradation of GLI1 by the proteasome.

Protein family/group databases

MEROPSiT01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta-5i
Gene namesi
Name:PSMB8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 23

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: UniProtKB-KW
  4. proteasome core complex Source: UniProtKB
  5. spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 7272Removed in mature formBy similarityPRO_0000239856Add
BLAST
Chaini73 – 276204Proteasome subunit beta type-8PRO_0000239857Add
BLAST

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity (By similarity).By similarity

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiQ3T112.

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level).

Gene expression databases

ExpressionAtlasiQ3T112. baseline and differential.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

Protein-protein interaction databases

IntActiQ3T112. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ3T112.
SMRiQ3T112. Positions 73-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiQ3T112.
KOiK02740.
OMAiSDLMHQY.
OrthoDBiEOG7FNC86.
TreeFamiTF106223.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3T112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLDVCGAP GGQRGDWAVP LAGSRQRSDP GHYGFSLRSP ELALPRGMQP
60 70 80 90 100
TEFFRSLGGN GESKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGNYIAT
110 120 130 140 150
LKVNKVIEIN PYLLGTMSGC AADCLYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMMC QYRGMGLSMG SMICGWDKKG PGLYYVDENG TRLSGNMFST
210 220 230 240 250
GSGNSHAYGV MDSGYRPDLS IEEAYDLGRR AIVHATHRDS YSGGVVNMYH
260 270
MKEDGWVKVE STDVSDLMHQ YREASQ
Length:276
Mass (Da):30,268
Last modified:June 13, 2006 - v2
Checksum:i5FE3755A1C1BF361
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112PG → TR in AAI02171. 1 PublicationCurated
Sequence conflicti42 – 421L → F in AAI02171. 1 PublicationCurated
Sequence conflicti99 – 991A → D in AAI02171. 1 PublicationCurated
Sequence conflicti125 – 1251L → Q in AAI02171. 1 PublicationCurated
Sequence conflicti187 – 1893DEN → NDS in AAI02171. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957499 Genomic DNA. Translation: AAY34697.1.
BC102170 mRNA. Translation: AAI02171.1.
RefSeqiNP_001035570.1. NM_001040480.2.
XP_005223251.1. XM_005223194.1.
UniGeneiBt.53697.

Genome annotation databases

EnsembliENSBTAT00000003955; ENSBTAP00000003955; ENSBTAG00000003039.
GeneIDi282013.
KEGGibta:282013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957499 Genomic DNA. Translation: AAY34697.1 .
BC102170 mRNA. Translation: AAI02171.1 .
RefSeqi NP_001035570.1. NM_001040480.2.
XP_005223251.1. XM_005223194.1.
UniGenei Bt.53697.

3D structure databases

ProteinModelPortali Q3T112.
SMRi Q3T112. Positions 73-271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3T112. 1 interaction.

Protein family/group databases

MEROPSi T01.015.

Proteomic databases

PRIDEi Q3T112.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000003955 ; ENSBTAP00000003955 ; ENSBTAG00000003039 .
GeneIDi 282013.
KEGGi bta:282013.

Organism-specific databases

CTDi 5696.

Phylogenomic databases

eggNOGi COG0638.
GeneTreei ENSGT00510000046395.
HOGENOMi HOG000091082.
HOVERGENi HBG108297.
InParanoidi Q3T112.
KOi K02740.
OMAi SDLMHQY.
OrthoDBi EOG7FNC86.
TreeFami TF106223.

Enzyme and pathway databases

Reactomei REACT_203739. Degradation of beta-catenin by the destruction complex.
REACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
REACT_205897. Activation of NF-kappaB in B cells.
REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
REACT_207857. Asymmetric localization of PCP proteins.
REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_208889. degradation of AXIN.
REACT_211738. ER-Phagosome pathway.
REACT_212887. Separation of Sister Chromatids.
REACT_213030. Orc1 removal from chromatin.
REACT_215163. degradation of DVL.
REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_237530. Regulation of ornithine decarboxylase (ODC).
REACT_252463. Hedgehog ligand biogenesis.
REACT_259250. Hh ligand biogenesis disease.
REACT_269173. Degradation of GLI2 by the proteasome.
REACT_269531. GLI3 is processed to GLI3R by the proteasome.
REACT_271133. Degradation of GLI1 by the proteasome.

Miscellaneous databases

NextBioi 20805879.

Gene expression databases

ExpressionAtlasi Q3T112. baseline and differential.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of the bovine MHC class IIb sequence identifies inversion breakpoints and three unexpected genes."
    Childers C.P., Newkirk H.L., Honeycutt D.A., Ramlachan N., Muzney D.M., Sodergren E., Gibbs R.A., Weinstock G.M., Womack J.E., Skow L.C.
    Anim. Genet. 37:121-129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiPSB8_BOVIN
AccessioniPrimary (citable) accession number: Q3T112
Secondary accession number(s): Q32S32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 26, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3