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Q3T112

- PSB8_BOVIN

UniProt

Q3T112 - PSB8_BOVIN

Protein

Proteasome subunit beta type-8

Gene

PSMB8

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity. Required for adipocyte differentiation By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei72 – 732Cleavage; by autocatalysisBy similarity
    Active sitei73 – 731NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. antigen processing and presentation Source: Ensembl
    2. fat cell differentiation Source: UniProtKB
    3. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Keywords - Biological processi

    Differentiation, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_205897. Activation of NF-kappaB in B cells.
    REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
    REACT_207857. Asymmetric localization of PCP proteins.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_208889. degradation of AXIN.
    REACT_211738. ER-Phagosome pathway.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213030. Orc1 removal from chromatin.
    REACT_215163. degradation of DVL.
    REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.

    Protein family/group databases

    MEROPSiT01.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-8 (EC:3.4.25.1)
    Alternative name(s):
    Proteasome subunit beta-5i
    Gene namesi
    Name:PSMB8
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 23

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB
    4. spermatoproteasome complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 7272Removed in mature formBy similarityPRO_0000239856Add
    BLAST
    Chaini73 – 276204Proteasome subunit beta type-8PRO_0000239857Add
    BLAST

    Post-translational modificationi

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PRIDEiQ3T112.

    Expressioni

    Inductioni

    Up-regulated by interferon gamma (at protein level).

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP.

    Protein-protein interaction databases

    IntActiQ3T112. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3T112.
    SMRiQ3T112. Positions 73-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00510000046395.
    HOGENOMiHOG000091082.
    HOVERGENiHBG108297.
    InParanoidiQ3T112.
    KOiK02740.
    OMAiSDLMHQY.
    OrthoDBiEOG7FNC86.
    TreeFamiTF106223.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3T112-1 [UniParc]FASTAAdd to Basket

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    MALLDVCGAP GGQRGDWAVP LAGSRQRSDP GHYGFSLRSP ELALPRGMQP    50
    TEFFRSLGGN GESKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGNYIAT 100
    LKVNKVIEIN PYLLGTMSGC AADCLYWERL LAKECRLYYL RNGERISVSA 150
    ASKLLSNMMC QYRGMGLSMG SMICGWDKKG PGLYYVDENG TRLSGNMFST 200
    GSGNSHAYGV MDSGYRPDLS IEEAYDLGRR AIVHATHRDS YSGGVVNMYH 250
    MKEDGWVKVE STDVSDLMHQ YREASQ 276
    Length:276
    Mass (Da):30,268
    Last modified:June 13, 2006 - v2
    Checksum:i5FE3755A1C1BF361
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 112PG → TR in AAI02171. 1 PublicationCurated
    Sequence conflicti42 – 421L → F in AAI02171. 1 PublicationCurated
    Sequence conflicti99 – 991A → D in AAI02171. 1 PublicationCurated
    Sequence conflicti125 – 1251L → Q in AAI02171. 1 PublicationCurated
    Sequence conflicti187 – 1893DEN → NDS in AAI02171. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY957499 Genomic DNA. Translation: AAY34697.1.
    BC102170 mRNA. Translation: AAI02171.1.
    RefSeqiNP_001035570.1. NM_001040480.2.
    XP_005223251.1. XM_005223194.1.
    UniGeneiBt.53697.

    Genome annotation databases

    EnsembliENSBTAT00000003955; ENSBTAP00000003955; ENSBTAG00000003039.
    GeneIDi282013.
    KEGGibta:282013.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY957499 Genomic DNA. Translation: AAY34697.1 .
    BC102170 mRNA. Translation: AAI02171.1 .
    RefSeqi NP_001035570.1. NM_001040480.2.
    XP_005223251.1. XM_005223194.1.
    UniGenei Bt.53697.

    3D structure databases

    ProteinModelPortali Q3T112.
    SMRi Q3T112. Positions 73-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3T112. 1 interaction.

    Protein family/group databases

    MEROPSi T01.015.

    Proteomic databases

    PRIDEi Q3T112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000003955 ; ENSBTAP00000003955 ; ENSBTAG00000003039 .
    GeneIDi 282013.
    KEGGi bta:282013.

    Organism-specific databases

    CTDi 5696.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00510000046395.
    HOGENOMi HOG000091082.
    HOVERGENi HBG108297.
    InParanoidi Q3T112.
    KOi K02740.
    OMAi SDLMHQY.
    OrthoDBi EOG7FNC86.
    TreeFami TF106223.

    Enzyme and pathway databases

    Reactomei REACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_205339. APC/C:Cdc20 mediated degradation of Securin.
    REACT_205897. Activation of NF-kappaB in B cells.
    REACT_206368. CDT1 association with the CDC6:ORC:origin complex.
    REACT_207857. Asymmetric localization of PCP proteins.
    REACT_208116. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_208851. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_208889. degradation of AXIN.
    REACT_211738. ER-Phagosome pathway.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213030. Orc1 removal from chromatin.
    REACT_215163. degradation of DVL.
    REACT_217860. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_218058. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_222557. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222622. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_223494. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_224434. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_225648. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_226120. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_227515. AUF1 (hnRNP D0) destabilizes mRNA.

    Miscellaneous databases

    NextBioi 20805879.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the bovine MHC class IIb sequence identifies inversion breakpoints and three unexpected genes."
      Childers C.P., Newkirk H.L., Honeycutt D.A., Ramlachan N., Muzney D.M., Sodergren E., Gibbs R.A., Weinstock G.M., Womack J.E., Skow L.C.
      Anim. Genet. 37:121-129(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.

    Entry informationi

    Entry nameiPSB8_BOVIN
    AccessioniPrimary (citable) accession number: Q3T112
    Secondary accession number(s): Q32S32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3