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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-1236974 ER-Phagosome pathway
R-BTA-421837 Clathrin derived vesicle budding
R-BTA-432720 Lysosome Vesicle Biogenesis
R-BTA-432722 Golgi Associated Vesicle Biogenesis
R-BTA-6798695 Neutrophil degranulation
R-BTA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-BTA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 8By similarity
Short name:
VAMP-8By similarity
Alternative name(s):
EndobrevinBy similarity
Short name:
EDBBy similarity
Gene namesi
Name:VAMP8By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Organism-specific databases

VGNCiVGNC:36760 VAMP8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
Transmembranei75 – 95Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini96 – 100VesicularSequence analysis5

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002737181 – 100Vesicle-associated membrane protein 8Add BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei27PhosphothreonineBy similarity1
Modified residuei47PhosphothreonineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei54PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3T0Y8
PRIDEiQ3T0Y8

Expressioni

Gene expression databases

BgeeiENSBTAG00000023997

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Interaction with STX8By similarity1

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000032965

Structurei

3D structure databases

ProteinModelPortaliQ3T0Y8
SMRiQ3T0Y8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 71v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiQ3T0Y8
KOiK08512
OMAiKMIVIIC
OrthoDBiEOG091G0MPE
TreeFamiTF320419

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Q3T0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASGGGGDD RVRNLRDEVE GVKNIMTQNV ERILARGENL DHLRNKTEDL
60 70 80 90 100
EATSEHFKTT SQKVARKFWW KNVKMIVLIC VIVFIIILFI VLFATGAIPT
Length:100
Mass (Da):11,357
Last modified:October 11, 2005 - v1
Checksum:iB117EA8A34E10701
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102203 mRNA Translation: AAI02204.1
RefSeqiNP_001029462.1, NM_001034290.2
XP_005212816.1, XM_005212759.3
UniGeneiBt.45766
Bt.91162

Genome annotation databases

EnsembliENSBTAT00000033040; ENSBTAP00000032965; ENSBTAG00000023997
GeneIDi507309
KEGGibta:507309

Similar proteinsi

Entry informationi

Entry nameiVAMP8_BOVIN
AccessioniPrimary (citable) accession number: Q3T0Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome