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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-1236974. ER-Phagosome pathway.
R-BTA-421837. Clathrin derived vesicle budding.
R-BTA-6798695. Neutrophil degranulation.
R-BTA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-BTA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 8By similarity
Short name:
VAMP-8By similarity
Alternative name(s):
EndobrevinBy similarity
Short name:
EDBBy similarity
Gene namesi
Name:VAMP8By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
Transmembranei75 – 95Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini96 – 100VesicularSequence analysis5

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002737181 – 100Vesicle-associated membrane protein 8Add BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei27PhosphothreonineBy similarity1
Modified residuei47PhosphothreonineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei54PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3T0Y8.
PRIDEiQ3T0Y8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000023997.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Interaction with STX8By similarity1

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000032965.

Structurei

3D structure databases

ProteinModelPortaliQ3T0Y8.
SMRiQ3T0Y8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 71v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ3T0Y8.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG091G0MPE.
TreeFamiTF320419.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3T0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASGGGGDD RVRNLRDEVE GVKNIMTQNV ERILARGENL DHLRNKTEDL
60 70 80 90 100
EATSEHFKTT SQKVARKFWW KNVKMIVLIC VIVFIIILFI VLFATGAIPT
Length:100
Mass (Da):11,357
Last modified:October 11, 2005 - v1
Checksum:iB117EA8A34E10701
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102203 mRNA. Translation: AAI02204.1.
RefSeqiNP_001029462.1. NM_001034290.2.
XP_005212816.1. XM_005212759.3.
UniGeneiBt.45766.
Bt.91162.

Genome annotation databases

EnsembliENSBTAT00000033040; ENSBTAP00000032965; ENSBTAG00000023997.
GeneIDi507309.
KEGGibta:507309.

Similar proteinsi

Entry informationi

Entry nameiVAMP8_BOVIN
AccessioniPrimary (citable) accession number: Q3T0Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: September 27, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families