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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-421837. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 8By similarity
Short name:
VAMP-8By similarity
Alternative name(s):
EndobrevinBy similarity
Short name:
EDBBy similarity
Gene namesi
Name:VAMP8By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

  • Lysosome membrane By similarity; Single-pass type IV membrane protein Curated
  • Late endosome membrane By similarity; Single-pass type IV membrane protein Curated
  • Early endosome membrane By similarity; Single-pass type IV membrane protein Curated
  • Cell membrane By similarity; Single-pass type IV membrane protein Curated

  • Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity). Sub-tight junctional domain in retinal pigment epithelium cells (By similarity). Midbody region during cytokinesis (By similarity). Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicSequence analysisAdd
BLAST
Transmembranei75 – 9521Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini96 – 1005VesicularSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100Vesicle-associated membrane protein 8PRO_0000273718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphothreonineBy similarity
Modified residuei54 – 541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3T0Y8.
PRIDEiQ3T0Y8.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Interaction with STX8By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000032965.

Structurei

3D structure databases

ProteinModelPortaliQ3T0Y8.
SMRiQ3T0Y8. Positions 11-63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ3T0Y8.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG75B87Z.
TreeFamiTF320419.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3T0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASGGGGDD RVRNLRDEVE GVKNIMTQNV ERILARGENL DHLRNKTEDL
60 70 80 90 100
EATSEHFKTT SQKVARKFWW KNVKMIVLIC VIVFIIILFI VLFATGAIPT
Length:100
Mass (Da):11,357
Last modified:October 11, 2005 - v1
Checksum:iB117EA8A34E10701
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102203 mRNA. Translation: AAI02204.1.
RefSeqiNP_001029462.1. NM_001034290.2.
XP_005212816.1. XM_005212759.3.
UniGeneiBt.45766.
Bt.91162.

Genome annotation databases

EnsembliENSBTAT00000033040; ENSBTAP00000032965; ENSBTAG00000023997.
GeneIDi507309.
KEGGibta:507309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102203 mRNA. Translation: AAI02204.1.
RefSeqiNP_001029462.1. NM_001034290.2.
XP_005212816.1. XM_005212759.3.
UniGeneiBt.45766.
Bt.91162.

3D structure databases

ProteinModelPortaliQ3T0Y8.
SMRiQ3T0Y8. Positions 11-63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000032965.

Proteomic databases

PaxDbiQ3T0Y8.
PRIDEiQ3T0Y8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000033040; ENSBTAP00000032965; ENSBTAG00000023997.
GeneIDi507309.
KEGGibta:507309.

Organism-specific databases

CTDi8673.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ3T0Y8.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG75B87Z.
TreeFamiTF320419.

Enzyme and pathway databases

ReactomeiR-BTA-421837. Clathrin derived vesicle budding.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiVAMP8_BOVIN
AccessioniPrimary (citable) accession number: Q3T0Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.