Q3T0X5 (PSA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-1
EC=3.4.25.1

Gene names

Name:

PSMA1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Biological process	Immunity
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from sequence or structural similarity. Source: UniProtKB proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular_function	lipopolysaccharide binding Inferred from electronic annotation. Source: Compara threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 263

263

Proteasome subunit alpha type-1

PRO_0000274031

Amino acid modifications

Modified residue

1

1

N-acetylmethionine By similarity

Modified residue

14

1

Phosphoserine By similarity

Glycosylation

110

1

O-linked (GlcNAc...) By similarity

Cross-link

115

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Cross-link

208

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Secondary structure

1

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263

Helix Strand Turn

Details...

Turn

5 – 7

3

Helix

20 – 31

12

Beta strand

35 – 39

5

Beta strand

41 – 49

9

Beta strand

63 – 67

5

Beta strand

70 – 76

7

Helix

78 – 99

22

Helix

105 – 121

17

Beta strand

130 – 138

9

Beta strand

141 – 147

7

Beta strand

149 – 151

3

Beta strand

153 – 162

10

Helix

165 – 179

15

Helix

184 – 196

13

Turn

207 – 209

3

Beta strand

210 – 216

7

Beta strand

219 – 224

6

Helix

227 – 229

3

Helix

230 – 233

4

Sequences

	Sequence		Length	Mass (Da)
	Q3T0X5 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 24142C5BCEFE8DED	FASTA	263	29,586
	10 20 30 40 50 60 MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK RAQSELAAHQ 70 80 90 100 110 120 KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD RPLPVSRLVS LIGSKTQIPT 130 140 150 160 170 180 QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS ANYFDCRAMS IGARSQSART YLERHMSEFM 190 200 210 220 230 240 ECNLNELVKH GLRALRETLP AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLEGLEERP 250 260 QRKAQPTQPA DEPAEKADEP MEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[2]	"The structure of the mammalian 20S proteasome at 2.75 A resolution." Unno M., Mizushima T., Morimoto Y., Tomisugi Y., Tanaka K., Yasuoka N., Tsukihara T. Structure 10:609-618(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF COMPLEX WITH THE 20S PROTEASOME.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC102216 mRNA. Translation: AAI02217.1.

IPI

IPI00687075.

RefSeq

NP_001030387.1. NM_001035310.2.

UniGene

Bt.23202.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IRU	X-ray	2.75	F/T	1-263	[»]

ProteinModelPortal

Q3T0X5.

SMR

Q3T0X5. Positions 4-241.

ModBase

Search...

Protein-protein interaction databases

STRING

9913.ENSBTAP00000008621.

Protein family/group databases

MEROPS

T01.976.

Proteomic databases

PaxDb

Q3T0X5.

PRIDE

Q3T0X5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000008621; ENSBTAP00000008621; ENSBTAG00000006564.

GeneID

515503.

KEGG

bta:515503.

Organism-specific databases

CTD

5682.

Phylogenomic databases

eggNOG

COG0638.

GeneTree

ENSGT00550000074855.

HOGENOM

HOG000091080.

HOVERGEN

HBG105373.

InParanoid

Q3T0X5.

KO

K02725.

OMA

RTYLERC.

OrthoDB

EOG4S1T7S.

Family and domain databases

InterPro

IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]

Pfam

PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]

SMART

SM00948. Proteasome_A_N. 1 hit.
[Graphical view]

PROSITE

PS00388. PROTEASOME_A_1. 1 hit.
PS51475. PROTEASOME_A_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q3T0X5.

NextBio

20871856.

Entry information

Entry name

PSA1_BOVIN

Accession

Primary (citable) accession number: Q3T0X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2005
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program