Reviewed, UniProtKB/Swiss-Prot Q3T0S5 (ALDOB_BOVIN)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase B
    EC=4.1.2.13
Alternative name(s):
    Liver-type aldolase

Gene names

Name:

ALDOB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Miscellaneous	In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 364	363	Fructose-bisphosphate aldolase B		PRO_0000284089
Sites
	Active site	188	1	Proton acceptor By similarity
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	364	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	36	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3T0S5-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 935BA56BC83353EA	FASTA	364	39,543
	10 20 30 40 50 60 MAHQFPALTS EQKKALSETA RRIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR 70 80 90 100 110 120 ELLFTVDSSV SQSIGGVILF HETLYQKDGQ GKLFRDILKE KGIVVGIKLD QGVAPLAGTN 130 140 150 160 170 180 KETTVQGLDG LSERCAQYKK DGADFGKWRA VLKIDNQCPS HLAIQENANT LARYASICQQ 190 200 210 220 230 240 NGLVPIVEPE VIPDGSHDME HCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC 250 260 270 280 290 300 TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF 310 320 330 340 350 360 SYGRALQASA LAAWGGKAEN KKTTQEAFMK RALANSQAAK GQYVHMGSSG SASTQSLFTA SYTY

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102278 mRNA. Translation: AAI02279.1.
IPI	IPI00686881.
RefSeq	NP_001029657.1.
UniGene	Bt.46035
3D structure databases
HSSP	HSSP built from PDB template 1QO5 based on UniProtKB P05062.
SMR	Q3T0S5. Positions 2-364.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3T0S5.
Proteomic databases
PRIDE	Q3T0S5.
Genome annotation databases
Ensembl	ENSBTAT00000020413; ENSBTAP00000020413; ENSBTAG00000015358; Bos taurus. [Genome view]
GeneID	515263.
KEGG	bta:515263.
Organism-specific databases
CTD	515263.
Phylogenomic databases
eggNOG	maNOG09308.
HOVERGEN	Q3T0S5.
InParanoid	Q3T0S5.
Enzyme and pathway databases
BRENDA	4.1.2.13. 251.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDOB_BOVIN

Accession

Primary (citable) accession number: Q3T0S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 11, 2005
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)