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Protein

3-ketoacyl-CoA thiolase, mitochondrial

Gene

ACAA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Abolishes BNIP3-mediated apoptosis and mitochondrial damage.By similarity

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Acyl-thioester intermediateBy similarity
Active sitei352 – 3521Proton acceptorPROSITE-ProRule annotation
Active sitei382 – 3821Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

SABIO-RKQ3T0R7.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase, mitochondrial (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Mitochondrial 3-oxoacyl-CoA thiolase
Gene namesi
Name:ACAA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

  • Mitochondrion By similarity

  • Note: Colocalizes with BNIP3 in the mitochondria.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3973973-ketoacyl-CoA thiolase, mitochondrialPRO_0000270498Add
BLAST
Transit peptidei1 – 1616Mitochondrion; not cleavedBy similarityAdd
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251N6-acetyllysine; alternateBy similarity
Modified residuei25 – 251N6-succinyllysine; alternateBy similarity
Modified residuei119 – 1191PhosphothreonineBy similarity
Modified residuei121 – 1211PhosphoserineBy similarity
Modified residuei127 – 1271PhosphotyrosineBy similarity
Modified residuei136 – 1361PhosphothreonineBy similarity
Modified residuei137 – 1371N6-acetyllysine; alternateBy similarity
Modified residuei137 – 1371N6-succinyllysine; alternateBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei143 – 1431N6-acetyllysine; alternateBy similarity
Modified residuei143 – 1431N6-succinyllysine; alternateBy similarity
Modified residuei171 – 1711N6-acetyllysine; alternateBy similarity
Modified residuei171 – 1711N6-succinyllysine; alternateBy similarity
Modified residuei191 – 1911N6-acetyllysine; alternateBy similarity
Modified residuei191 – 1911N6-succinyllysine; alternateBy similarity
Modified residuei209 – 2091N6-acetyllysine; alternateBy similarity
Modified residuei209 – 2091N6-succinyllysine; alternateBy similarity
Modified residuei212 – 2121N6-succinyllysineBy similarity
Modified residuei214 – 2141N6-succinyllysineBy similarity
Modified residuei234 – 2341N6-acetyllysine; alternateBy similarity
Modified residuei234 – 2341N6-succinyllysine; alternateBy similarity
Modified residuei240 – 2401N6-succinyllysineBy similarity
Modified residuei241 – 2411N6-acetyllysineBy similarity
Modified residuei269 – 2691N6-acetyllysineBy similarity
Modified residuei270 – 2701N6-acetyllysineBy similarity
Modified residuei305 – 3051N6-acetyllysine; alternateBy similarity
Modified residuei305 – 3051N6-succinyllysine; alternateBy similarity
Modified residuei310 – 3101PhosphoserineBy similarity
Modified residuei312 – 3121N6-acetyllysine; alternateBy similarity
Modified residuei312 – 3121N6-succinyllysine; alternateBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei340 – 3401N6-acetyllysineBy similarity
Modified residuei375 – 3751N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3T0R7.
PRIDEiQ3T0R7.

Interactioni

Subunit structurei

Homotetramer. Interacts with BNIP3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3T0R7. 1 interaction.
STRINGi9913.ENSBTAP00000003716.

Structurei

3D structure databases

ProteinModelPortaliQ3T0R7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ3T0R7.
KOiK07508.
OMAiCQEICVK.
OrthoDBiEOG7JQBNG.
TreeFamiTF105696.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3T0R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLRGVFIV AAKRTPFGAY GGLLKDFTPT DMAEFAARAA LSAGRVSPET
60 70 80 90 100
VDSVVVGNVM QSSSDAIYLA RHVGLRVGIP KETPAITINR LCGSGFQSIV
110 120 130 140 150
SGCQEICSRD SEVVLCGGTE SMSQAPYCVR NIRFGTKLGS ELKLEDTLWT
160 170 180 190 200
GLTDTHVQMP MAITAENLAV KHQISREDCD RYALQSQQRW KTANDAGYFD
210 220 230 240 250
NEMAPVEVKT RKGKQTMQVD EHPRPQTTME QLNKLPPVFK KEGTVTAGNA
260 270 280 290 300
SGVSDGAGAV IIASEDAVKK HNFTPLARIV GYFVSGCDPT IMGIGPVPAI
310 320 330 340 350
SGALKKTGLS LKDMDLVEVN EAFAPQYLAV EKSLNLDPSK TNVNGGAIAL
360 370 380 390
GHPLAGSGSR ITAHLVHELR RRGGKYAVGS ACIGGGQGIA VIIENTA
Length:397
Mass (Da):42,131
Last modified:October 11, 2005 - v1
Checksum:iD1AFAAA509594E11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF583004 mRNA. Translation: ABU62844.1.
EF583005 Genomic DNA. Translation: ABU62845.1.
BT025480 mRNA. Translation: ABF57436.1.
BC102287 mRNA. Translation: AAI02288.1.
RefSeqiNP_001030419.1. NM_001035342.2.
UniGeneiBt.48847.

Genome annotation databases

EnsembliENSBTAT00000003716; ENSBTAP00000003716; ENSBTAG00000002863.
GeneIDi522006.
KEGGibta:522006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF583004 mRNA. Translation: ABU62844.1.
EF583005 Genomic DNA. Translation: ABU62845.1.
BT025480 mRNA. Translation: ABF57436.1.
BC102287 mRNA. Translation: AAI02288.1.
RefSeqiNP_001030419.1. NM_001035342.2.
UniGeneiBt.48847.

3D structure databases

ProteinModelPortaliQ3T0R7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3T0R7. 1 interaction.
STRINGi9913.ENSBTAP00000003716.

Proteomic databases

PaxDbiQ3T0R7.
PRIDEiQ3T0R7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000003716; ENSBTAP00000003716; ENSBTAG00000002863.
GeneIDi522006.
KEGGibta:522006.

Organism-specific databases

CTDi10449.

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ3T0R7.
KOiK07508.
OMAiCQEICVK.
OrthoDBiEOG7JQBNG.
TreeFamiTF105696.

Enzyme and pathway databases

UniPathwayiUPA00199.
SABIO-RKQ3T0R7.

Miscellaneous databases

NextBioi20873423.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Association analysis of bovine ACAA2 and economic traits."
    Li H., Xu S., Gao X.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiTHIM_BOVIN
AccessioniPrimary (citable) accession number: Q3T0R7
Secondary accession number(s): B4X9P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 11, 2005
Last modified: November 11, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.