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Protein

Nucleoside diphosphate kinase B

Gene

NME2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC (By similarity). Exhibits histidine protein kinase activity.By similarity1 Publication

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATPBy similarity1
Binding sitei60ATPBy similarity1
Binding sitei88ATPBy similarity1
Binding sitei94ATPBy similarity1
Binding sitei105ATPBy similarity1
Binding sitei115ATPBy similarity1
Active sitei118Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
Histidine protein kinase NDKB (EC:2.7.13.3)
Gene namesi
Name:NME2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002501991 – 152Nucleoside diphosphate kinase BAdd BLAST152

Proteomic databases

PaxDbiQ3T0Q4.
PeptideAtlasiQ3T0Q4.
PRIDEiQ3T0Q4.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8. Interacts ITGB1BP1 (via C-terminal domain region) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GNB1P628713EBI-8577979,EBI-357141

Protein-protein interaction databases

CORUMiQ3T0Q4.
IntActiQ3T0Q4. 1 interactor.
STRINGi9913.ENSBTAP00000041066.

Structurei

3D structure databases

ProteinModelPortaliQ3T0Q4.
SMRiQ3T0Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 66Interaction with AKAP13By similarityAdd BLAST66

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiQ3T0Q4.
KOiK00940.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiView protein in InterPro
IPR034907. NDK-like_dom.
IPR036850. NDK-like_dom_sf.
IPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
PfamiView protein in Pfam
PF00334. NDK. 1 hit.
PRINTSiPR01243. NUCDPKINASE.
SMARTiView protein in SMART
SM00562. NDK. 1 hit.
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiView protein in PROSITE
PS00469. NDP_KINASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3T0Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHAERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LQASEELLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI NLWFKPEELI EYKPCAFDWI

YE
Length:152
Mass (Da):17,316
Last modified:October 11, 2005 - v1
Checksum:iC3EB0D24AEFA4183
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102300 mRNA. Translation: AAI02301.1.
RefSeqiNP_001069844.1. NM_001076376.1.
UniGeneiBt.64945.

Genome annotation databases

GeneIDi615447.
KEGGibta:615447.

Similar proteinsi

Entry informationi

Entry nameiNDKB_BOVIN
AccessioniPrimary (citable) accession number: Q3T0Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 11, 2005
Last modified: October 25, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families