Nucleoside diphosphate kinase B - Bos taurus (Bovine)

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Q3T0Q4 (NDKB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 53. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase B
Short name=NDK B
Short name=NDP kinase B
EC=2.7.4.6

Alternative name(s):
Histidine protein kinase NDKB
EC=2.7.13.3

Gene names

Name:

NME2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC By similarity. Exhibits histidine protein kinase activity. Ref.2
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Cofactor	Magnesium By similarity.
Subunit structure	Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8 By similarity.
Subcellular location	Cytoplasm. Cell membrane By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC protein histidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 152

151

Nucleoside diphosphate kinase B

PRO_0000250199

Regions

Region

2 – 66

Interaction with AKAP13 By similarity

Sites

Active site

118

Pros-phosphohistidine intermediate By similarity

Binding site

ATP By similarity

Binding site

ATP By similarity

Binding site

ATP By similarity

Binding site

ATP By similarity

Binding site

105

ATP By similarity

Binding site

115

ATP By similarity

Amino acid modifications

Modified residue

N-acetylalanine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

Phosphothreonine By similarity

Modified residue

100

N6-acetyllysine By similarity

Modified residue

124

N6-acetyllysine By similarity

Modified residue

128

N6-acetyllysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q3T0Q4 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: C3EB0D24AEFA4183
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FASTA

152

17,316

        10         20         30         40         50         60 
MAHAERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LQASEELLKQ HYIDLKDRPF 

        70         80         90        100        110        120 
FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 

       130        140        150 
DSVKSAEKEI NLWFKPEELI EYKPCAFDWI YE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[2]	"Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta." Cuello F., Schulze R.A., Heemeyer F., Meyer H.E., Lutz S., Jakobs K.H., Niroomand F., Wieland T. J. Biol. Chem. 278:7220-7226(2003) [PubMed: 12486123] [Abstract] Cited for: FUNCTION AS HISTIDINE PROTEIN KINASE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102300 mRNA. Translation: AAI02301.1.
IPI	IPI00903886.
RefSeq	NP_001069844.1. NM_001076376.1.
UniGene	Bt.64945.
3D structure databases
HSSP	HSSP built from PDB template 1NSK based on UniProtKB P22392.
ProteinModelPortal	Q3T0Q4.
SMR	Q3T0Q4. Positions 2-152.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3T0Q4.
Proteomic databases
PRIDE	Q3T0Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	615447.
KEGG	bta:615447.
Organism-specific databases
CTD	4831.
Phylogenomic databases
eggNOG	maNOG10260.
GeneTree	ENSGT00550000074206.
HOVERGEN	HBG000423.
InParanoid	Q3T0Q4.
OrthoDB	EOG4S1T8G.
PhylomeDB	Q3T0Q4.
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
KO	K00940.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_BOVIN

Accession

Primary (citable) accession number: Q3T0Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	October 11, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents