Reviewed, UniProtKB/Swiss-Prot Q3T0Q0 (SAT1_BOVIN)

Names and origin

Protein names

Recommended name:
    Diamine acetyltransferase 1
    EC=2.3.1.57
Alternative name(s):
    Spermidine/spermine N(1)-acetyltransferase 1
      Short name=SSAT-1
      Short name=SSAT
    Putrescine acetyltransferase
    Polyamine N-acetyltransferase 1

Gene names

Name:	SAT1
Synonyms:	SAT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine By similarity.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Diamine acetyltransferase 1		PRO_0000244026
Regions
	Domain	4 – 170	167	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	126 – 128	3	Substrate binding By similarity
	Region	133 – 136	4	Acetyl-CoA binding By similarity
	Region	140 – 143	4	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	26	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3T0Q0-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 516CA1D5EB91F466	FASTA	171	19,925
	10 20 30 40 50 60 MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVMLTEKD LLEDGFGEHP FYHCLVAEVP 70 80 90 100 110 120 KEHLTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KKLSQVAMKC 130 140 150 160 170 RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMAAE E

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102304 mRNA. Translation: AAI02305.1.
IPI	IPI00695374.
RefSeq	NP_001029505.1.
UniGene	Bt.7594
3D structure databases
SMR	Q3T0Q0. Positions 2-171.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023087; ENSBTAP00000023087; ENSBTAG00000017363; Bos taurus. [Genome view]
GeneID	508861.
KEGG	bta:508861.
Organism-specific databases
CTD	508861.
Phylogenomic databases
HOVERGEN	Q3T0Q0.
Enzyme and pathway databases
BRENDA	2.3.1.57. 251.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT1_BOVIN

Accession

Primary (citable) accession number: Q3T0Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 11, 2005
Last modified:	October 13, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)