Reviewed, UniProtKB/Swiss-Prot Q3T0I2 (CATH_BOVIN)

Names and origin

Protein names

Recommended name:
    Cathepsin H
    EC=3.4.22.16
Cleaved into the following 3 chains:
    1- Recommended name:
            Cathepsin H mini chain
    2- Recommended name:
            Cathepsin H heavy chain
    3- Recommended name:
            Cathepsin H light chain

Gene names

Name:

CTSH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes By similarity.
Catalytic activity	Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.
Subunit structure	Composed of a mini chain and a large chain. The large chain may be split into heavy and light chain. All chains are held together by disulfide bonds By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Propeptide	23 – 97	75	Activation peptide By similarity		PRO_0000238112
	Peptide	98 – 105	8	Cathepsin H mini chain		PRO_0000238113
	Propeptide	106 – 115	10	By similarity		PRO_0000238114
	Chain	116 – 335	220	Cathepsin H		PRO_0000238115
	Chain	116 – 292	177	Cathepsin H heavy chain		PRO_0000238116
	Chain	293 – 335	43	Cathepsin H light chain		PRO_0000238117
Sites
	Active site	141	1	By similarity
	Active site	281	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Disulfide bond	102 ↔ 327		By similarity
	Disulfide bond	138 ↔ 181		By similarity
	Disulfide bond	172 ↔ 214		By similarity
	Disulfide bond	272 ↔ 322		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3T0I2-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 79FDBEBB9984D227	FASTA	335	37,351
	10 20 30 40 50 60 MWAVLPLLCA GAWLLGAPAC GAAELAANSL EKFHFQSWMV QHQKKYSSEE YYHRLQAFAS 70 80 90 100 110 120 NLREINAHNA RNHTFKMGLN QFSDMSFDEL KRKYLWSEPQ NCSATKSNYL RGTGPYPPSM 130 140 150 160 170 180 DWRKKGNFVT PVKNQGSCGS CWTFSTTGAL ESAVAIATGK LPFLAEQQLV DCAQNFNNHG 190 200 210 220 230 240 CQGGLPSQAF EYIRYNKGIM GEDTYPYRGQ DGDCKYQPSK AIAFVKDVAN ITLNDEEAMV 250 260 270 280 290 300 EAVALHNPVS FAFEVTADFM MYRKGIYSST SCHKTPDKVN HAVLAVGYGE EKGIPYWIVK 310 320 330 NSWGPNWGMK GYFLIERGKN MCGLAACASF PIPLV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102386 mRNA. Translation: AAI02387.1.
IPI	IPI00693034.
RefSeq	NP_001029557.1.
UniGene	Bt.52393
3D structure databases
SMR	Q3T0I2. Positions 116-335.
ModBase	Search...
Protein family/group databases
MEROPS	C01.040.
Genome annotation databases
Ensembl	ENSBTAG00000010992. Bos taurus. [Contig view]
GeneID	510524.
KEGG	bta:510524.
Phylogenomic databases
HOVERGEN	Q3T0I2.
OMA	Q3T0I2. HHRLQTF.
Enzyme and pathway databases
BRENDA	3.4.22.16. 251.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATH_BOVIN

Accession

Primary (citable) accession number: Q3T0I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 11, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)