Q3T046 (BDH2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxybutyrate dehydrogenase type 2
EC=1.1.1.-
EC=1.1.1.30

Alternative name(s):
Dehydrogenase/reductase SDR family member 6
R-beta-hydroxybutyrate dehydrogenase

Gene names

Name:	BDH2
Synonyms:	DHRS6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase By similarity.
Catalytic activity	(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.
Pathway	Siderophore biosynthesis.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme metabolic process Inferred from sequence or structural similarity. Source: UniProtKB iron ion homeostasis Inferred from sequence or structural similarity. Source: UniProtKB siderophore biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	3-hydroxybutyrate dehydrogenase type 2		PRO_0000247551
Regions
	Nucleotide binding	10 – 37	28	NAD By similarity
	Nucleotide binding	180 – 184	5	NAD By similarity
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	58	1	NAD By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	151	1	NAD By similarity
	Binding site	188	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
Amino acid modifications
	Modified residue	48	1	N6-acetyllysine By similarity
	Modified residue	132	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3T046 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: A846933ACBA6F83C	FASTA	245	26,662
	10 20 30 40 50 60 MGRLDGKVIV LTAAAQGIGR AAALAFAKEG AKVIATDIND SKLQELDKYP GIHTRVLDVT 70 80 90 100 110 120 KKKQIDQFAN DIERLDVLFN VAGFVHHGTI LDCEETDWDF SMNLNVRSMY LMIKAFLPKM 130 140 150 160 170 180 MAQKSGNIIN MSSVASSIKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV 190 200 210 220 230 240 DTPSLQERIQ ARPNPEEALS DFLKRQKTGR FATAEEVALL CVYLASDESA YITGNPVIID GGWSL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102567 mRNA. Translation: AAI02568.1.
IPI	IPI00694312.
RefSeq	NP_001029660.1. NM_001034488.1.
UniGene	Bt.1792.
3D structure databases
ProteinModelPortal	Q3T046.
SMR	Q3T046. Positions 1-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3T046.
Proteomic databases
PRIDE	Q3T046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	515321.
KEGG	bta:515321.
Organism-specific databases
CTD	56898.
Phylogenomic databases
eggNOG	maNOG04096.
GeneTree	ENSGT00600000084182.
HOVERGEN	HBG002145.
PhylomeDB	Q3T046.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00019.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BDH2_BOVIN

Accession

Primary (citable) accession number: Q3T046

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 11, 2005
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program