Reviewed, UniProtKB/Swiss-Prot Q3T001 (H17B6_BOVIN)

Names and origin

Protein names

Recommended name:
    Hydroxysteroid 17-beta dehydrogenase 6
    EC=1.1.1.62
    EC=1.1.1.63
    EC=1.1.1.105
Alternative name(s):
    17-beta-HSD6
    Oxidative 3-alpha hydroxysteroid dehydrogenase
    3-alpha->beta-hydroxysteroid epimerase
      Short name=3-alpha->beta-HSE

Gene names

Name:

HSD17B6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH. Retinol + NAD(+) = retinal + NADH.
Subcellular location	Microsome membrane; Peripheral membrane protein; Lumenal sideBy similarity. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal sideBy similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC retinol dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 317	300	Hydroxysteroid 17-beta dehydrogenase 6		PRO_0000303210
Regions
	Nucleotide binding	33 – 57	25	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	164	1	Substrate Potential
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3T001-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 4B1A2ED7906FBB12	FASTA	317	36,198
	10 20 30 40 50 60 MWLYLAVLLG LYYLLRWFRE RQVVSHLQDK FVFITGCDSG FGNQLARQLD LRGLRVLAGC 70 80 90 100 110 120 LTEQGAEQLR NQTSDRLQTV ILDVTKTESI AAATEWVKEC VGDRGLWGLV NNAGIFHSHG 130 140 150 160 170 180 YAEWIKIETY RDTLRVNLIG VIEVTLSMLP LVRKAQGRIV NVSSILGRIA FFGAVYSCSK 190 200 210 220 230 240 YGVEAFSDIL RRELQHFGVK VSMVEPGYFR TAMTDWQKFS EIMKQIWKET PAHIKETYGQ 250 260 270 280 290 300 KFFDAYHDLM KQGLLSCSTN LNLVTDCMEH ALTSVHPRTR YSAGWDAQFF FVPLSYLPTS 310 LADYILTRSW PKPAQAV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102626 mRNA. Translation: AAI02627.1.
RefSeq	NP_001030472.1.
UniGene	Bt.16385
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011720. Bos taurus. [Contig view]
GeneID	533086.
KEGG	bta:533086.
Phylogenomic databases
HOVERGEN	Q3T001.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H17B6_BOVIN

Accession

Primary (citable) accession number: Q3T001

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 11, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)