Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interferon regulatory factor 1

Gene

IRF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58/RIG-I, TNFSF10/TRAIL, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells (By similarity).By similarity

Enzyme regulationi

Activated by MYD88.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 113IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST109

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 1
Short name:
IRF-1
Gene namesi
Name:IRF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002236741 – 322Interferon regulatory factor 1Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysineBy similarity1
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki296Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Phosphorylated by CK2 and this positively regulates its activity.By similarity
Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-276. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites (By similarity).By similarity
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3SZP0.
PRIDEiQ3SZP0.

Expressioni

Gene expression databases

BgeeiENSBTAG00000031231.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with EP300, PIAS3 and MYD88 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041732.

Structurei

3D structure databases

ProteinModelPortaliQ3SZP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IER3. Eukaryota.
ENOG4111HM7. LUCA.
HOGENOMiHOG000037937.
HOVERGENiHBG003455.
InParanoidiQ3SZP0.
KOiK09444.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR031215. IRF1.
IPR017431. IRF1/IRF2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11949:SF3. PTHR11949:SF3. 1 hit.
PfamiPF00605. IRF. 1 hit.
[Graphical view]
PIRSFiPIRSF038196. IFN_RF1/2. 1 hit.
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SZP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK
60 70 80 90 100
DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN
110 120 130 140 150
KGSSAVRVYR MLPPLTKSQR KERKSKSSRD ARSKAKKKPY GEYSPDTFSD
160 170 180 190 200
GLSSSTLPDD HSNYTVRSYM GQDLDIERTL TPALSPCGVS STLPNWSIPV
210 220 230 240 250
EIVPDSTSDL YNFQVSPMPS TSEAATDEDE EGKLTEDIMK LLEQTGWQQT
260 270 280 290 300
SVDGKGYLLN EPGAQPTSVY GEFSCKEEPE VDSPGGYIGL ISSDMKNMDP
310 320
SLLDSLLTPV RLPSIQAIPC AP
Length:322
Mass (Da):36,168
Last modified:October 11, 2005 - v1
Checksum:i273CA27DAE5DB7BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302L → W in AAO46998 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY204899 mRNA. Translation: AAO46998.1.
BC102766 mRNA. Translation: AAI02767.1.
RefSeqiNP_001178190.1. NM_001191261.2.
UniGeneiBt.102576.
Bt.87587.
Bt.89067.

Genome annotation databases

GeneIDi789216.
KEGGibta:789216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY204899 mRNA. Translation: AAO46998.1.
BC102766 mRNA. Translation: AAI02767.1.
RefSeqiNP_001178190.1. NM_001191261.2.
UniGeneiBt.102576.
Bt.87587.
Bt.89067.

3D structure databases

ProteinModelPortaliQ3SZP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041732.

Proteomic databases

PaxDbiQ3SZP0.
PRIDEiQ3SZP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi789216.
KEGGibta:789216.

Organism-specific databases

CTDi3659.

Phylogenomic databases

eggNOGiENOG410IER3. Eukaryota.
ENOG4111HM7. LUCA.
HOGENOMiHOG000037937.
HOVERGENiHBG003455.
InParanoidiQ3SZP0.
KOiK09444.
TreeFamiTF328512.

Gene expression databases

BgeeiENSBTAG00000031231.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR031215. IRF1.
IPR017431. IRF1/IRF2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11949:SF3. PTHR11949:SF3. 1 hit.
PfamiPF00605. IRF. 1 hit.
[Graphical view]
PIRSFiPIRSF038196. IFN_RF1/2. 1 hit.
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIRF1_BOVIN
AccessioniPrimary (citable) accession number: Q3SZP0
Secondary accession number(s): Q865L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 11, 2005
Last modified: October 5, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.