Reviewed, UniProtKB/Swiss-Prot Q3SZM9 (NAGK_BOVIN)

Names and origin

Protein names

Recommended name:
    N-acetyl-D-glucosamine kinase
      Short name=N-acetylglucosamine kinase
    EC=2.7.1.59
Alternative name(s):
    GlcNAc kinase

Gene names

Name:

NAGK

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity By similarity.
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	N-acetyl-D-glucosamine kinase		PRO_0000253473
Regions
	Nucleotide binding	6 – 13	8	ATP By similarity
	Region	129 – 130	2	Substrate binding By similarity
	Region	145 – 147	3	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	271	1	ATP By similarity
	Binding site	275	1	ATP By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	205	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZM9-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 575EC6F41FA4BA08	FASTA	344	37,268
	10 20 30 40 50 60 MVTLYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTDKCVERIN EMVNRAKRKA 70 80 90 100 110 120 GVDPLVPLRG LGLSLSGGDQ EDAVRMLMEE LRDRFPYLSE SYLITTDAAG SIATATPDGG 130 140 150 160 170 180 VVLISGTGSN CRLINPDGSE SGCGGWGHMM GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD 190 200 210 220 230 240 IGYVKQAMFN YFQVPDRLGI LTHLYRDFDK SRFAGFCRKV AEGAQQGDPL SRCIFRKAGE 250 260 270 280 290 300 MLGRHVVAVL PEIDPVLFQG EMGLPILCVG SVWKSWELLK EGFLLALTQG REIQAQNSFS 310 320 330 340 GFTLLKLRHS SALGAASLGA KHIGHLLPMD YSTSAIAFYS YTFS

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102780 mRNA. Translation: AAI02781.1.
IPI	IPI00718059.
RefSeq	NP_001029486.1.
UniGene	Bt.4387
3D structure databases
SMR	Q3SZM9. Positions 1-344.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000014668. Bos taurus. [Contig view]
GeneID	508132.
KEGG	bta:508132.
Phylogenomic databases
HOVERGEN	Q3SZM9.
OMA	Q3SZM9. RDRFPYL.
Enzyme and pathway databases
BRENDA	2.7.1.59. 251.
Family and domain databases
InterPro	IPR002731. ATPase_BadF. [Graphical view]
Pfam	PF01869. BcrAD_BadFG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_BOVIN

Accession

Primary (citable) accession number: Q3SZM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 11, 2005
Last modified:	May 5, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)