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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Gene

SLC9A3R1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Short name:
NHERF-1
Alternative name(s):
Ezrin-radixin-moesin-binding phosphoprotein 50
Short name:
EBP50
Regulatory cofactor of Na(+)/H(+) exchanger
Sodium-hydrogen exchanger regulatory factor 1
Solute carrier family 9 isoform A3 regulatory factor 1
Gene namesi
Name:SLC9A3R1
Synonyms:NHERF, NHERF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 368367Na(+)/H(+) exchange regulatory cofactor NHE-RF1PRO_0000301264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineBy similarity
Modified residuei288 – 2881PhosphoserineBy similarity
Modified residuei299 – 2991PhosphoserineBy similarity
Modified residuei301 – 3011PhosphothreonineBy similarity
Modified residuei302 – 3021PhosphoserineBy similarity
Modified residuei307 – 3071PhosphoserineBy similarity
Modified residuei310 – 3101PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3SZK8.
PRIDEiQ3SZK8.

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1. Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1. Directly interacts with HTR4. Interacts with MCC. Interacts with TRPC4 (via the PDZ-binding domain). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting. Interacts (via the PDZ domains) with SLC26A6. Interacts with SLC26A3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034568.

Structurei

3D structure databases

ProteinModelPortaliQ3SZK8.
SMRiQ3SZK8. Positions 11-99, 158-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9481PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini162 – 24281PDZ 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KDNH. Eukaryota.
ENOG4110SZZ. LUCA.
GeneTreeiENSGT00530000062999.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ3SZK8.
KOiK13365.
OMAiAMKKGPN.
OrthoDBiEOG7R56T6.
TreeFamiTF350449.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR031199. NHERF-1.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF7. PTHR14191:SF7. 1 hit.
PfamiPF09007. EBP50_C. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADAGAGAP LPRLCCLEKG PNGYGFHLHG EKGKVGQYIR LVEPGSPAEK
60 70 80 90 100
SGLLAGDRLV EVNGENVEKE THQQVVNRIR AALNSVRLLV VDPETDERLQ
110 120 130 140 150
KLGVQVREEM LRAQEGPGQA EPPAAAAEER GAGGENEPPA AAPEPREAEQ
160 170 180 190 200
SPQERRELRP RLCAMKKGPN GYGFNLHSDK SKPGQFIRAV DPDSPAEASG
210 220 230 240 250
LRAQDRIVEV NGVCVEGKPH GEVVSAIKAG GDEAKLLVVD RETDEFFKKC
260 270 280 290 300
KVIPSQEHLQ GPLPEPITNG EIEKENSPEA LAETASESPM PPLARTASSD
310 320 330 340 350
TSEELNSQDS PKKQDSTAPS STSSSSSDPV LDFSISLAVA KERAHQKRVS
360
KRAPQMDWSK KNELFSNL
Length:368
Mass (Da):39,603
Last modified:October 11, 2005 - v1
Checksum:i8215AB11957F3395
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102807 mRNA. Translation: AAI02808.1.
RefSeqiNP_001071320.1. NM_001077852.2.
UniGeneiBt.44195.

Genome annotation databases

EnsembliENSBTAT00000034682; ENSBTAP00000034568; ENSBTAG00000011382.
GeneIDi505242.
KEGGibta:505242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102807 mRNA. Translation: AAI02808.1.
RefSeqiNP_001071320.1. NM_001077852.2.
UniGeneiBt.44195.

3D structure databases

ProteinModelPortaliQ3SZK8.
SMRiQ3SZK8. Positions 11-99, 158-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034568.

Proteomic databases

PaxDbiQ3SZK8.
PRIDEiQ3SZK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000034682; ENSBTAP00000034568; ENSBTAG00000011382.
GeneIDi505242.
KEGGibta:505242.

Organism-specific databases

CTDi9368.

Phylogenomic databases

eggNOGiENOG410KDNH. Eukaryota.
ENOG4110SZZ. LUCA.
GeneTreeiENSGT00530000062999.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ3SZK8.
KOiK13365.
OMAiAMKKGPN.
OrthoDBiEOG7R56T6.
TreeFamiTF350449.

Miscellaneous databases

NextBioi20867049.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR031199. NHERF-1.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF7. PTHR14191:SF7. 1 hit.
PfamiPF09007. EBP50_C. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiNHRF1_BOVIN
AccessioniPrimary (citable) accession number: Q3SZK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 11, 2005
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.