ID   PTGR1_BOVIN             Reviewed;         329 AA.
AC   Q3SZJ4;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=Prostaglandin reductase 1;
DE            Short=PRG-1;
DE            EC=1.3.1.-;
DE   AltName: Full=NADP-dependent leukotriene B4 12-hydroxydehydrogenase;
DE            EC=1.3.1.74;
DE   AltName: Full=15-oxoprostaglandin 13-reductase;
DE            EC=1.3.1.48;
GN   Name=PTGR1; Synonyms=LTB4DH;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions as 15-oxo-prostaglandin 13-reductase and acts
CC       on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity
CC       towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of
CC       leukotriene B4 into its biologically less active metabolite, 12-
CC       oxo-leukotriene B4. This is an initial and key step of metabolic
CC       inactivation of leukotriene B4 (By similarity).
CC   -!- CATALYTIC ACTIVITY: n-alkanal + NAD(P)(+) = alk-2-enal + NAD(P)H.
CC   -!- CATALYTIC ACTIVITY: 11-alpha-hydroxy-9,15-dioxoprost-5-enoate +
CC       NAD(P)(+) = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-
CC       dienoate + NAD(P)H.
CC   -!- SUBUNIT: Monomer or homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the NADP-dependent oxidoreductase L4BD
CC       family.
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DR   EMBL; BC102822; AAI02823.1; -; mRNA.
DR   IPI; IPI00706362; -.
DR   RefSeq; NP_001030358.1; -.
DR   UniGene; Bt.51592; -.
DR   SMR; Q3SZJ4; 1-329.
DR   Ensembl; ENSBTAG00000011027; Bos taurus.
DR   GeneID; 513177; -.
DR   KEGG; bta:513177; -.
DR   HOVERGEN; Q3SZJ4; -.
DR   BRENDA; 1.3.1.48; 251.
DR   BRENDA; 1.3.1.74; 251.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047522; F:15-oxoprostaglandin 13-oxidase activity; IEA:EC.
DR   GO; GO:0032440; F:2-alkenal reductase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002085; ADH_SF_Zn.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR014190; B4_12hDH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   TIGRFAMs; TIGR02825; B4_12hDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; NADP; Oxidoreductase.
FT   CHAIN         1    329       Prostaglandin reductase 1.
FT                                /FTId=PRO_0000285790.
FT   NP_BIND     149    166       NADP (Potential).
FT   BINDING     178    178       NADP (By similarity).
FT   BINDING     193    193       NADP (By similarity).
FT   BINDING     217    217       NADP (By similarity).
FT   BINDING     245    245       NADP (By similarity).
FT   BINDING     321    321       NADP (By similarity).
SQ   SEQUENCE   329 AA;  35706 MW;  8CCF8128A6447C33 CRC64;
     MVHAKSWTLK KHFVGYPTNS DFELKTVELP PLKDGEVLLE ALYLTVDPYM RIMAKSLKEG
     DMMMGEQVAR VVESKNSAFP TGTIVLAPSG WTTHSISNGE KLEKVLAEWP DTLPLSLALG
     TVGMPGLTAY FGLLDICGVK GGETVLVSAA AGAVGSIVGQ IAKLKGCKVV GTAGSDEKVA
     WLKKHGFDVA LNYKTVKSLE EALKEAAPEG YDCYFDNVGG EFSNVAITQM KKFGRIAICG
     AISVYNRTSP LSPGPSPEII IFKELHLQGF VVYRWQGEVR QKALRDLLKW VSEGKIQYHE
     HVTEGFENMP AAFIGLLKGE NLGKAIVKA
//