Reviewed, UniProtKB/Swiss-Prot Q3SZJ4 (PTGR1_BOVIN)

Names and origin

Protein names

Recommended name:
    Prostaglandin reductase 1
      Short name=PRG-1
    EC=1.3.1.-
Alternative name(s):
    NADP-dependent leukotriene B4 12-hydroxydehydrogenase
    EC=1.3.1.74
    15-oxoprostaglandin 13-reductase
    EC=1.3.1.48

Gene names

Name:	PTGR1
Synonyms:	LTB4DH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 By similarity.
Catalytic activity	n-alkanal + NAD(P)(+) = alk-2-enal + NAD(P)H. 11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)(+) = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Subunit structure	Monomer or homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from electronic annotation. Source: EC 2-alkenal reductase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Prostaglandin reductase 1		PRO_0000285790
Regions
	Nucleotide binding	149 – 166	18	NADP Potential
Sites
	Binding site	178	1	NADP By similarity
	Binding site	193	1	NADP By similarity
	Binding site	217	1	NADP By similarity
	Binding site	245	1	NADP By similarity
	Binding site	321	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZJ4-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 8CCF8128A6447C33	FASTA	329	35,706
	10 20 30 40 50 60 MVHAKSWTLK KHFVGYPTNS DFELKTVELP PLKDGEVLLE ALYLTVDPYM RIMAKSLKEG 70 80 90 100 110 120 DMMMGEQVAR VVESKNSAFP TGTIVLAPSG WTTHSISNGE KLEKVLAEWP DTLPLSLALG 130 140 150 160 170 180 TVGMPGLTAY FGLLDICGVK GGETVLVSAA AGAVGSIVGQ IAKLKGCKVV GTAGSDEKVA 190 200 210 220 230 240 WLKKHGFDVA LNYKTVKSLE EALKEAAPEG YDCYFDNVGG EFSNVAITQM KKFGRIAICG 250 260 270 280 290 300 AISVYNRTSP LSPGPSPEII IFKELHLQGF VVYRWQGEVR QKALRDLLKW VSEGKIQYHE 310 320 HVTEGFENMP AAFIGLLKGE NLGKAIVKA

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102822 mRNA. Translation: AAI02823.1.
RefSeq	NP_001030358.1.
UniGene	Bt.51592
3D structure databases
SMR	Q3SZJ4. Positions 1-329.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011027. Bos taurus. [Contig view]
GeneID	513177.
KEGG	bta:513177.
Phylogenomic databases
HOVERGEN	Q3SZJ4.
Family and domain databases
InterPro	IPR002085. AlcDHase_SF_Zn. IPR013149. AlcDHase_Zn-bd. IPR014190. B4_12hDHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02825. B4_12hDH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTGR1_BOVIN

Accession

Primary (citable) accession number: Q3SZJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 11, 2005
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)