Q3SZJ0 (ARLY_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:

ASL

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.
Enzyme regulation	Enzyme activity is regulated by acetylation By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
Subunit structure	Homotetramer By similarity.
Post-translational modification	Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TCA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity By similarity.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Urea cycle
Molecular function	Lyase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro internal protein amino acid acetylation Inferred from sequence or structural similarity. Source: UniProtKB urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Argininosuccinate lyase		PRO_0000273253
Amino acid modifications
	Modified residue	69	1	N6-acetyllysine By similarity
	Modified residue	288	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZJ0 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: DCF05530CFB06404	FASTA	473	52,743
	10 20 30 40 50 60 MASESGKLWG GRFVGTVDPI MEKFNSSITY DRHLWEADVQ GSKAYSRGLE KAGLLTKAEM 70 80 90 100 110 120 DQILHGLDKV AEEWAQGTFK LNPNDEDIHT ANERRLKELI GETAGKLHTG RSRNDQVVTD 130 140 150 160 170 180 LRLWMRQNCS MLSALLCELI RTMVDRAEAE RDVLFPGYTH LQRAQPIRWS HWILSHAVAL 190 200 210 220 230 240 TRDSERLLEV RKRINVLPLG SGAIAGNPLG VDRELLRAEL DFGAITLNSM DATSERDFVA 250 260 270 280 290 300 EFLFWASLCM THLSRMAEDL ILYGTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA 310 320 330 340 350 360 GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMS AVLQVATGVI STLQIHRENM 370 380 390 400 410 420 GRALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET KGVALNQLSL QELQTISPLF 430 440 450 460 470 SGDVSHVWDY GHSVEQYEAL GGTARSSVDW QIGQLRALLR AQQTESPPHA SPK

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102831 mRNA. Translation: AAI02832.1.
IPI	IPI00703946.
RefSeq	NP_001029600.1. NM_001034428.1.
UniGene	Bt.10013.
3D structure databases
HSSP	HSSP built from PDB template 1XWO based on UniProtKB P33110.
ProteinModelPortal	Q3SZJ0.
SMR	Q3SZJ0. Positions 6-463.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SZJ0.
Proteomic databases
PRIDE	Q3SZJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	512771.
KEGG	bta:512771.
Organism-specific databases
CTD	435.
Phylogenomic databases
HOVERGEN	HBG004281.
InParanoid	Q3SZJ0.
OrthoDB	EOG418BN7.
PhylomeDB	Q3SZJ0.
Family and domain databases
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. ArgH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY_BOVIN

Accession

Primary (citable) accession number: Q3SZJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2005
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program