Reviewed, UniProtKB/Swiss-Prot Q3SZI6 (RPN2_BOVIN)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
    Ribophorin-2
    Ribophorin II
      Short name=RPN-II

Gene names

Name:

RPN2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SWP1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
PTM	Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 631	609	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2		PRO_0000328635
Regions
	Topological domain	23 – 540	518	Lumenal Potential
	Transmembrane	541 – 561	21	Potential
	Transmembrane	572 – 592	21	Potential
	Transmembrane	597 – 617	21	Potential
	Topological domain	618 – 631	14	Cytoplasmic Potential
	Compositional bias	256 – 261	6	Poly-Ala
	Compositional bias	269 – 274	6	Poly-Val
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Cross-link	154		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZI6-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 006BFB6A50A246F1	FASTA	631	69,214
	10 20 30 40 50 60 MALPGSSTVF LLALTIIAST QALTPTHYLT KHDVERLKAS LDRPFTSLES AFYSIVGLSS 70 80 90 100 110 120 LGAQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQALSGC EISISNETKD LLLAAVSEDS 130 140 150 160 170 180 SVAQIYHAVA ALSGFGLPLA SQEALGALTA RLSKEETVLA TVQALQTASY LSQQADLRSI 190 200 210 220 230 240 VEEIEDLVAR LDELGGVYLQ FEEGLETTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNAI 250 260 270 280 290 300 FSKKNFESLS EAFSVASAAA ALSENRYHVP VVVVPEGSPS YTQEQAILRL QVTNVLSQPL 310 320 330 340 350 360 TQATVKLEHA KSVASRATVL QKTSFTLIGD VFELNFMNVK FSSGYYDFSV KVEGDNRYIA 370 380 390 400 410 420 NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA KAKGTFIADS HQNFALFFQL 430 440 450 460 470 480 VDVNTGAELT PHQTFVRLHN QKTGQEVVFV AEPDSKNVYK FELDTSERKL EFDSASGTYT 490 500 510 520 530 540 LYLIIGDATL KNPILWNVAD VVIRFPEDDV PSTVLSKNIF TPKQEIQHLF REPEKRPPTV 550 560 570 580 590 600 VSNTFTALIL SPLLLLFALW IRIGANVSNF TFAPSTIVFH LGHAAMLGLM YVYWTQLNMF 610 620 630 QTLKYLAILG SVTFLAGNRM LAQQAIKRTA H

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT030513 mRNA. Translation: ABQ12953.1. BC102838 mRNA. Translation: AAI02839.1.
IPI	IPI00693977.
RefSeq	NP_001029776.1.
UniGene	Bt.48878
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SZI6.
Genome annotation databases
Ensembl	ENSBTAT00000057162; ENSBTAP00000051209; ENSBTAG00000014648; Bos taurus. [Genome view]
GeneID	534231.
KEGG	bta:534231.
Organism-specific databases
CTD	534231.
Phylogenomic databases
eggNOG	maNOG16585.
HOVERGEN	Q3SZI6.
InParanoid	Q3SZI6.
Enzyme and pathway databases
BRENDA	2.4.1.119. 251.
Family and domain databases
InterPro	IPR008814. Ribophorin_II. [Graphical view]
PANTHER	PTHR12640. Ribophorin_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPN2_BOVIN

Accession

Primary (citable) accession number: Q3SZI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 11, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)