Reviewed, UniProtKB/Swiss-Prot Q3SZI0 (MPI_BOVIN)

Names and origin

Protein names

Recommended name:
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase

Gene names

Name:

MPI

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 423	422	Mannose-6-phosphate isomerase		PRO_0000245030
Sites
	Active site	295	1	By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Metal binding	276	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZI0-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9BE8AB8D3B8B37E5	FASTA	423	46,369
	10 20 30 40 50 60 MAAQRVFPLS CVVQQYAWGK MGSNSEVARL LASSDPLAQI SEDRPYAELW MGTHPRGDAK 70 80 90 100 110 120 ILDNRISQKT LGQWIADNQD SLGSKVKDTF NGKLPFLFKV LSVETALSIQ AHPNKELAEK 130 140 150 160 170 180 LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LTKVPEFQFL IGDNAAAQLK 190 200 210 220 230 240 QSLSQDSEAV TSALRSCFSH LMKSEKKVVV EQLNLLVKRI SQQVAAGNNM EDICGELLLQ 250 260 270 280 290 300 LHQQYPGDIG CFAIYFLNLL TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP 310 320 330 340 350 360 KFIDVPTLCE MLSYTPSPSQ DRLFPPARSP EDPYLSIYDP PVPDFTVMKV EVPGSVTEYK 370 380 390 400 410 420 VLALDSASIL LVVQGTVTAS SPTAQAAIPL KRGGVLFIGA NESVSLKLTV PKDLLMFRAC CLL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102844 mRNA. Translation: AAI02845.1.
IPI	IPI00692165.
RefSeq	NP_001030361.1.
UniGene	Bt.48873
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000005845. Bos taurus. [Contig view]
GeneID	513586.
KEGG	bta:513586.
Phylogenomic databases
HOVERGEN	Q3SZI0.
Enzyme and pathway databases
BRENDA	5.3.1.8. 251.
Family and domain databases
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_BOVIN

Accession

Primary (citable) accession number: Q3SZI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 35 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)