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Protein

Mannose-6-phosphate isomerase

Gene

MPI

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (MPI), Mannose-6-phosphate isomerase (MPI)
  2. Phosphomannomutase (PMM2), Phosphomannomutase 2 (PMM2), Phosphomannomutase (PMM1)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincBy similarity1
Metal bindingi112ZincBy similarity1
Metal bindingi137ZincBy similarity1
Metal bindingi276ZincBy similarity1
Active sitei295By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:MPI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002450302 – 423Mannose-6-phosphate isomeraseAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei108PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ3SZI0.

Structurei

3D structure databases

ProteinModelPortaliQ3SZI0.
SMRiQ3SZI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000241277.
HOVERGENiHBG000367.
InParanoidiQ3SZI0.
KOiK01809.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiView protein in InterPro
IPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin_sf.
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiView protein in Pfam
PF01238. PMI_typeI. 1 hit.
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiView protein in PROSITE
PS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQRVFPLS CVVQQYAWGK MGSNSEVARL LASSDPLAQI SEDRPYAELW
60 70 80 90 100
MGTHPRGDAK ILDNRISQKT LGQWIADNQD SLGSKVKDTF NGKLPFLFKV
110 120 130 140 150
LSVETALSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG
160 170 180 190 200
FRPVEEIVTF LTKVPEFQFL IGDNAAAQLK QSLSQDSEAV TSALRSCFSH
210 220 230 240 250
LMKSEKKVVV EQLNLLVKRI SQQVAAGNNM EDICGELLLQ LHQQYPGDIG
260 270 280 290 300
CFAIYFLNLL TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP
310 320 330 340 350
KFIDVPTLCE MLSYTPSPSQ DRLFPPARSP EDPYLSIYDP PVPDFTVMKV
360 370 380 390 400
EVPGSVTEYK VLALDSASIL LVVQGTVTAS SPTAQAAIPL KRGGVLFIGA
410 420
NESVSLKLTV PKDLLMFRAC CLL
Length:423
Mass (Da):46,369
Last modified:January 23, 2007 - v3
Checksum:i9BE8AB8D3B8B37E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102844 mRNA. Translation: AAI02845.1.
RefSeqiNP_001030361.1. NM_001035284.1.
UniGeneiBt.48873.

Genome annotation databases

GeneIDi513586.
KEGGibta:513586.

Similar proteinsi

Entry informationi

Entry nameiMPI_BOVIN
AccessioniPrimary (citable) accession number: Q3SZI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 90 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families