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Q3SZD7 (CBR1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Carbonyl reductase [NADPH] 1
EC=1.1.1.184
Alternative name(s):
15-hydroxyprostaglandin dehydrogenase [NADP(+)]
EC=1.1.1.197
NADPH-dependent carbonyl reductase 1
Prostaglandin 9-ketoreductase
Prostaglandin-E(2) 9-reductase
EC=1.1.1.189
Gene names
Name:CBR1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione By similarity.

Catalytic activity

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

(5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH.

(13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP+ = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 277276Carbonyl reductase [NADPH] 1
PRO_0000284143

Regions

Nucleotide binding10 – 3425NADP By similarity
Nucleotide binding63 – 642NADP By similarity
Nucleotide binding194 – 1985NADP By similarity
Nucleotide binding231 – 2333NADP By similarity
Region95 – 973Glutathione binding By similarity
Region193 – 1942Glutathione binding By similarity

Sites

Active site1941Proton acceptor By similarity
Binding site901NADP; via carbonyl oxygen By similarity
Binding site1061Glutathione By similarity
Binding site1401Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue2391N6-1-carboxyethyl lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SZD7 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 04B641F931BCD6DF

FASTA27730,533
        10         20         30         40         50         60 
MSSSNCVALV TGANKGIGFV IVRDLCRRFS GDVVLTARDE ARGRAAVQQL QAEGLSPLFH 

        70         80         90        100        110        120 
QLDIDDRQSI RALRDFLRKE YGGLDVLVNN AGIAFKTADT TPFHIQAEVT MKTNFFGTRD 

       130        140        150        160        170        180 
VCTELLPLIK PQGRVVNVSS FVSVNSLKKC SRELQQKFRS ETITEEELVG LMNKFVEDTK 

       190        200        210        220        230        240 
NGVHRKEGWP DTAYGVTKIG VTVLSRIHAR KLSEQRGGDK ILLNACCPGW VRTDMGGPKA 

       250        260        270 
SKSPEEGAET PVYLALLPSD AEGPHGEFIS EKRVVQW

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC102943 mRNA. Translation: AAI02944.1.
IPIIPI00708761.
RefSeqNP_001029685.1. NM_001034513.1.
UniGeneBt.7872.

3D structure databases

ProteinModelPortalQ3SZD7.
SMRQ3SZD7. Positions 7-277.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000019311.

Proteomic databases

PaxDbQ3SZD7.
PRIDEQ3SZD7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID515946.
KEGGbta:515946.

Organism-specific databases

CTD873.

Phylogenomic databases

eggNOGCOG1028.
HOVERGENHBG001909.
KOK00079.
OrthoDBEOG4BP1CB.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20872066.

Entry information

Entry nameCBR1_BOVIN
AccessionPrimary (citable) accession number: Q3SZD7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 11, 2005
Last modified: April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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