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Protein

Proteasome subunit beta type-9

Gene

PSMB9

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 212Cleavage; by autolysisBy similarity
Active sitei21 – 211NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiREACT_274004. degradation of DVL.
REACT_276477. ER-Phagosome pathway.
REACT_280521. Activation of NF-kappaB in B cells.
REACT_282220. Asymmetric localization of PCP proteins.
REACT_290505. SCF(Skp2)-mediated degradation of p27/p21.
REACT_291730. Separation of Sister Chromatids.
REACT_293325. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_295102. CDT1 association with the CDC6:ORC:origin complex.
REACT_297209. Degradation of beta-catenin by the destruction complex.
REACT_302851. CDK-mediated phosphorylation and removal of Cdc6.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_306095. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_307496. Hedgehog ligand biogenesis.
REACT_308391. Ubiquitin-dependent degradation of Cyclin D1.
REACT_311810. Hedgehog 'on' state.
REACT_314321. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_321728. Regulation of ornithine decarboxylase (ODC).
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_327729. Degradation of GLI2 by the proteasome.
REACT_328795. Orc1 removal from chromatin.
REACT_329551. degradation of AXIN.
REACT_329646. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_330101. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_333090. GLI3 is processed to GLI3R by the proteasome.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_350460. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_357698. Dectin-1 mediated noncanonical NF-kB signaling.
REACT_358073. Degradation of GLI1 by the proteasome.
REACT_362031. CLEC7A (Dectin-1) signaling.

Protein family/group databases

MEROPSiT01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-9 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta-1i
Gene namesi
Name:PSMB9
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 23

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2020Removed in mature formBy similarityPRO_0000239860Add
BLAST
Chaini21 – 219199Proteasome subunit beta type-9PRO_0000239861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity
Modified residuei109 – 1091N6-acetyllysineBy similarity

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Keywords - PTMi

Acetylation, Zymogen

Proteomic databases

PRIDEiQ3SZC2.

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level).

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis.

Protein-protein interaction databases

IntActiQ3SZC2. 1 interaction.
STRINGi9913.ENSBTAP00000011789.

Structurei

3D structure databases

ProteinModelPortaliQ3SZC2.
SMRiQ3SZC2. Positions 21-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG291022.
GeneTreeiENSGT00510000046484.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiQ3SZC2.
KOiK02741.
OMAiQVYGTMG.
OrthoDBiEOG79GT80.
TreeFamiTF106221.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTGAPNGD LPRAGEVHTG TTIMAVEFDG GVVVGSDSRV SAGEAVVNRV
60 70 80 90 100
FDKLSPLHQH IYCALSGSAA DAQAIADMAA YQLELHGMEL EEPPLVLAAA
110 120 130 140 150
NVVRNITYKY REDLSAHLMV AGWDQREGGQ VYGTMSGMLI RQPFAIGGSG
160 170 180 190 200
STYIYGYVDA AYKPGMSPEE CRRFTTNAIA LAMKRDGSSG GVIYLATITG
210
AGVDHRVILG DELPRFYDE
Length:219
Mass (Da):23,404
Last modified:October 11, 2005 - v1
Checksum:i16388C96B02E28C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957499 Genomic DNA. Translation: AAY34699.1.
BC102963 mRNA. Translation: AAI02964.1.
RefSeqiNP_001029560.1. NM_001034388.2.
UniGeneiBt.48919.

Genome annotation databases

EnsembliENSBTAT00000011789; ENSBTAP00000011789; ENSBTAG00000008954.
GeneIDi510593.
KEGGibta:510593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957499 Genomic DNA. Translation: AAY34699.1.
BC102963 mRNA. Translation: AAI02964.1.
RefSeqiNP_001029560.1. NM_001034388.2.
UniGeneiBt.48919.

3D structure databases

ProteinModelPortaliQ3SZC2.
SMRiQ3SZC2. Positions 21-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3SZC2. 1 interaction.
STRINGi9913.ENSBTAP00000011789.

Protein family/group databases

MEROPSiT01.013.

Proteomic databases

PRIDEiQ3SZC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011789; ENSBTAP00000011789; ENSBTAG00000008954.
GeneIDi510593.
KEGGibta:510593.

Organism-specific databases

CTDi5698.

Phylogenomic databases

eggNOGiNOG291022.
GeneTreeiENSGT00510000046484.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiQ3SZC2.
KOiK02741.
OMAiQVYGTMG.
OrthoDBiEOG79GT80.
TreeFamiTF106221.

Enzyme and pathway databases

ReactomeiREACT_274004. degradation of DVL.
REACT_276477. ER-Phagosome pathway.
REACT_280521. Activation of NF-kappaB in B cells.
REACT_282220. Asymmetric localization of PCP proteins.
REACT_290505. SCF(Skp2)-mediated degradation of p27/p21.
REACT_291730. Separation of Sister Chromatids.
REACT_293325. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_295102. CDT1 association with the CDC6:ORC:origin complex.
REACT_297209. Degradation of beta-catenin by the destruction complex.
REACT_302851. CDK-mediated phosphorylation and removal of Cdc6.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_306095. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_307496. Hedgehog ligand biogenesis.
REACT_308391. Ubiquitin-dependent degradation of Cyclin D1.
REACT_311810. Hedgehog 'on' state.
REACT_314321. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_321728. Regulation of ornithine decarboxylase (ODC).
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_327729. Degradation of GLI2 by the proteasome.
REACT_328795. Orc1 removal from chromatin.
REACT_329551. degradation of AXIN.
REACT_329646. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_330101. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_333090. GLI3 is processed to GLI3R by the proteasome.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_350460. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_357698. Dectin-1 mediated noncanonical NF-kB signaling.
REACT_358073. Degradation of GLI1 by the proteasome.
REACT_362031. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

NextBioi20869526.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative analysis of the bovine MHC class IIb sequence identifies inversion breakpoints and three unexpected genes."
    Childers C.P., Newkirk H.L., Honeycutt D.A., Ramlachan N., Muzney D.M., Sodergren E., Gibbs R.A., Weinstock G.M., Womack J.E., Skow L.C.
    Anim. Genet. 37:121-129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiPSB9_BOVIN
AccessioniPrimary (citable) accession number: Q3SZC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 11, 2005
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded in the MHC class II region.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.