Reviewed, UniProtKB/Swiss-Prot Q3SZB7 (F16P1_BOVIN)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase 1
      Short name=FBPase 1
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase 1

Gene names

Name:

FBP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. AMP binding affects the turnover of bound substrate and not the affinity for substrate. Fructose-2,6-biphosphate acts as competitive inhibitor. Fructose-2,6-biphosphate and AMP have synergistic effects By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Gluconeogenesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 338	337	Fructose-1,6-bisphosphatase 1		PRO_0000247322
Regions
	Nucleotide binding	18 – 22	5	AMP By similarity
	Nucleotide binding	28 – 32	5	AMP By similarity
	Nucleotide binding	113 – 114	2	AMP By similarity
	Region	122 – 125	4	Substrate binding By similarity
	Region	213 – 216	4	Substrate binding By similarity
	Region	244 – 249	6	Substrate binding By similarity
	Region	275 – 277	3	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 3 By similarity
	Metal binding	121	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	122	1	Magnesium 3 By similarity
	Metal binding	281	1	Magnesium 3 By similarity
	Binding site	141	1	AMP
	Binding site	265	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZB7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 83EB68040BC2664F	FASTA	338	36,728
	10 20 30 40 50 60 MTDQAAFDTN IVTVTRFVME EGRKARGTGE MTQLLNSLCT AVKAISTAVR KAGIAHLYGI 70 80 90 100 110 120 AGTTNVTGDQ VKKLDVLSND LVVNVLKSSF ATCVLVSEED EHAIIVEPEK RGKYVVCFDP 130 140 150 160 170 180 LDGSSNIDCL VSIGTIFGIY KKISKDDPSE KDALQPGRNL VAAGYALYGS ATMLVLAMAN 190 200 210 220 230 240 GVNCFMLDPA IGEFILVDRD VKIKKKGSIY SLNEGYAKDF DPALTEYVQR KKFPPDNSAP 250 260 270 280 290 300 YGARYVGSMV ADVHRTLVYG GIFMYPANKK SPSGKLRLLY ECNPMAYVIE KAGGMATTGK 310 320 330 ETVLDIVPTD IHQKSPIILG SPEDVTEFLE IYKKHAAK

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102974 mRNA. Translation: AAI02975.1.
IPI	IPI00704584.
RefSeq	NP_001029619.1.
UniGene	Bt.24314
3D structure databases
SMR	Q3SZB7. Positions 7-338.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SZB7.
Proteomic databases
PRIDE	Q3SZB7.
Genome annotation databases
Ensembl	ENSBTAT00000012835; ENSBTAP00000012835; ENSBTAG00000009733; Bos taurus. [Genome view]
GeneID	513483.
KEGG	bta:513483.
Organism-specific databases
CTD	513483.
Phylogenomic databases
eggNOG	maNOG08767.
HOVERGEN	Q3SZB7.
InParanoid	Q3SZB7.
PhylomeDB	Q3SZB7.
Enzyme and pathway databases
BRENDA	3.1.3.11. 251.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P1_BOVIN

Accession

Primary (citable) accession number: Q3SZB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 41 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)