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Protein

Fructose-1,6-bisphosphatase 1

Gene

FBP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain (By similarity).By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. AMP binding affects the turnover of bound substrate and not the affinity for substrate. Fructose 2,6-bisphosphate acts as competitive inhibitor. Fructose 2,6-bisphosphate and AMP have synergistic effects (By similarity).By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Magnesium 1By similarity
Metal bindingi98 – 981Magnesium 1By similarity
Metal bindingi98 – 981Magnesium 2By similarity
Metal bindingi119 – 1191Magnesium 2By similarity
Metal bindingi119 – 1191Magnesium 3By similarity
Metal bindingi121 – 1211Magnesium 2; via carbonyl oxygenBy similarity
Metal bindingi122 – 1221Magnesium 3By similarity
Binding sitei141 – 1411AMP
Binding sitei265 – 2651SubstrateBy similarity
Metal bindingi281 – 2811Magnesium 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 225AMPBy similarity
Nucleotide bindingi28 – 325AMPBy similarity
Nucleotide bindingi113 – 1142AMPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Gluconeogenesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-70263. Gluconeogenesis.
SABIO-RKQ3SZB7.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase 1 (EC:3.1.3.11)
Short name:
FBPase 1
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase 1
Liver FBPase
Gene namesi
Name:FBP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 338337Fructose-1,6-bisphosphatase 1PRO_0000247322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei151 – 1511N6-succinyllysineBy similarity
Modified residuei208 – 2081PhosphoserineBy similarity
Modified residuei216 – 2161PhosphotyrosineBy similarity
Modified residuei245 – 2451PhosphotyrosineBy similarity
Modified residuei265 – 2651PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3SZB7.
PRIDEiQ3SZB7.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012835.

Structurei

3D structure databases

ProteinModelPortaliQ3SZB7.
SMRiQ3SZB7. Positions 7-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1254Substrate bindingBy similarity
Regioni213 – 2164Substrate bindingBy similarity
Regioni244 – 2496Substrate bindingBy similarity
Regioni275 – 2773Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
GeneTreeiENSGT00390000015513.
HOGENOMiHOG000191265.
HOVERGENiHBG005627.
InParanoidiQ3SZB7.
KOiK03841.
OMAiEANPMSW.
OrthoDBiEOG7GJ6D9.
TreeFamiTF314824.

Family and domain databases

HAMAPiMF_01855. FBPase_class1.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQAAFDTN IVTVTRFVME EGRKARGTGE MTQLLNSLCT AVKAISTAVR
60 70 80 90 100
KAGIAHLYGI AGTTNVTGDQ VKKLDVLSND LVVNVLKSSF ATCVLVSEED
110 120 130 140 150
EHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSIGTIFGIY KKISKDDPSE
160 170 180 190 200
KDALQPGRNL VAAGYALYGS ATMLVLAMAN GVNCFMLDPA IGEFILVDRD
210 220 230 240 250
VKIKKKGSIY SLNEGYAKDF DPALTEYVQR KKFPPDNSAP YGARYVGSMV
260 270 280 290 300
ADVHRTLVYG GIFMYPANKK SPSGKLRLLY ECNPMAYVIE KAGGMATTGK
310 320 330
ETVLDIVPTD IHQKSPIILG SPEDVTEFLE IYKKHAAK
Length:338
Mass (Da):36,728
Last modified:January 23, 2007 - v3
Checksum:i83EB68040BC2664F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102974 mRNA. Translation: AAI02975.1.
RefSeqiNP_001029619.1. NM_001034447.2.
UniGeneiBt.24314.

Genome annotation databases

EnsembliENSBTAT00000012835; ENSBTAP00000012835; ENSBTAG00000009733.
GeneIDi513483.
KEGGibta:513483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102974 mRNA. Translation: AAI02975.1.
RefSeqiNP_001029619.1. NM_001034447.2.
UniGeneiBt.24314.

3D structure databases

ProteinModelPortaliQ3SZB7.
SMRiQ3SZB7. Positions 7-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012835.

Proteomic databases

PaxDbiQ3SZB7.
PRIDEiQ3SZB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012835; ENSBTAP00000012835; ENSBTAG00000009733.
GeneIDi513483.
KEGGibta:513483.

Organism-specific databases

CTDi2203.

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
GeneTreeiENSGT00390000015513.
HOGENOMiHOG000191265.
HOVERGENiHBG005627.
InParanoidiQ3SZB7.
KOiK03841.
OMAiEANPMSW.
OrthoDBiEOG7GJ6D9.
TreeFamiTF314824.

Enzyme and pathway databases

UniPathwayiUPA00138.
ReactomeiR-BTA-70263. Gluconeogenesis.
SABIO-RKQ3SZB7.

Family and domain databases

HAMAPiMF_01855. FBPase_class1.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiF16P1_BOVIN
AccessioniPrimary (citable) accession number: Q3SZB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.